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Selenium Supplementation Alters Gene Expression Profiles Associated with Innate Immunity in Whole-Blood Neutrophils of Sheep

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Abstract

Footrot (FR) is a common, contagious bacterial disease of sheep that results in lameness and significant economic losses for producers. We previously reported that sheep affected with FR have lower whole-blood (WB) selenium (Se) concentrations and that Se supplementation in conjunction with routine control practices accelerates recovery from FR. To determine whether oral Se-yeast administered at supranutritional levels (>4.9 mg Se/week) alters the ability of sheep to resist or recover from FR infection, 60 ewes with and 60 ewes without FR were drenched once weekly for 62.5 weeks with 0, 4.9, 14.7, or 24.5 mg organic Se-yeast (30 ewes per treatment group). Footrot prevalence and severity were measured at 0, 20, 28, 40, and 60 weeks of Se supplementation. Genomic expression of eight WB-neutrophil genes for selenoproteins and seven WB-neutrophil genes for proteins involved in innate immunity was determined at the end of the treatment period using SYBR Green and quantitative polymerase chain reaction methodology. Supranutritional Se-yeast supplementation successfully increased Se status in sheep but did not prevent FR. Supranutritional Se-yeast supplementation increased WB-neutrophil expression of genes involved in innate immunity: l-selectin, interleukin-8 receptor, and toll-like receptor 4, which were or tended to be lower in ewes affected with FR. Furthermore, supranutritional Se-yeast supplementation altered the expression of selenoprotein genes involved in innate immunity, increasing selenoprotein S and glutathione peroxidase 4 and decreasing iodothyronine deiodinases 2 and 3. In conclusion, supranutritional Se-yeast supplementation does not prevent FR, but does alter WB-neutrophil gene expression profiles associated with innate immunity, including reversing those impacted by FR.

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Abbreviations

COX II:

Cyclooxygenase II

Dio:

Iodothyronine deiodinases

FBS:

Fetal bovine serum

FOS:

FBJ murine osteosarcoma viral oncogene homolog or c-FOS, and a member of the activator protein-1 family

FR:

Footrot

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

GPx:

Glutathione peroxidases

HBSS:

Hank's balanced saline solution

ICP-MS:

Ionized coupled plasma mass spectrometry

IFN-γ:

Interferon-gamma

IL-8R:

Interleukin-8 receptor

l-Sel:

l-Selectin

NFκB:

Nuclear factor kappa B

PBS:

Phosphate buffered saline

PPM1A:

Protein phosphatase magnesium-dependent 1A

qPCR:

Quantitative polymerase chain reaction

RBC:

Red blood cells

ROS:

Reactive oxygen species

RPL19:

Ribosomal protein large subunit family member-19

Se:

Selenium

SeCys:

Selenocysteine

SeMet:

Selenomethionine

SEP:

Selenoproteins

TBK1:

TANK-binding kinase 1

TLR4:

Toll-like receptor 4

TxnR:

Thioredoxin reductase

WB:

Whole blood

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Acknowledgments

The authors thank Thomas Nichols, Sheep Center Director, Department of Animal and Rangeland Sciences, College of Agricultural Sciences, Oregon State University, for hoof examination, trimming, and foot scoring for FR. This study was funded in part by USDA CSREES 2008-35204-04624, Agricultural Research Foundation, and Animal Health and Disease Project Formula Funds, Oregon State University, Corvallis, OR.

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  1. Hugejiletu Hugejiletu

    Present address: College of Veterinary Medicine, Inner Mongolian Agricultural University, Hohhot, Inner Mongolia, 010018, China

Authors and Affiliations

  1. Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR, 97331, USA

    Hugejiletu Hugejiletu, William R. Vorachek, M. Elena Gorman & Jean A. Hall

  2. Department of Animal and Rangeland Sciences, College of Agricultural Sciences, Oregon State University, Corvallis, OR, 97331, USA

    Gerd Bobe, Wayne D. Mosher & Gene J. Pirelli

  3. Linus Pauling Institute, Oregon State University, Corvallis, OR, 97331, USA

    Gerd Bobe

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Hugejiletu, H., Bobe, G., Vorachek, W.R. et al. Selenium Supplementation Alters Gene Expression Profiles Associated with Innate Immunity in Whole-Blood Neutrophils of Sheep. Biol Trace Elem Res 154, 28–44 (2013). https://doi.org/10.1007/s12011-013-9716-6

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  • DOI: https://doi.org/10.1007/s12011-013-9716-6

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