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Ferritin Levels and Hepcidin mRNA Expression in Peripheral Mononuclear Cells from Anemic Type 2 Diabetic Patients

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Abstract

This study aims to measure iron nutrition parameters and to determine the presence of anemia in obese type 2 diabetic patients and to analyze the mRNA relative abundance of genes related to inflammation, immune system, iron metabolism, and mitochondrial activity. Obese type 2 diabetic (OBDM, n = 30) and healthy subjects (Cn, n = 30) were studied. Biochemical, anthropometric, and iron nutrition parameters were determined. Peripheral mononuclear cells from type 2 diabetic and control group were challenged with high concentrations of iron (Fe) and glucose and total mRNA was isolated. The frequency of anemia among diabetic patients was 4/30. OBDM patients with or without anemia had higher levels of ferritin and high-sensitivity C-reactive protein than the Cn group. mRNA relative abundance of nuclear factor kappa-light-chain-enhancer of activated B cells was elevated in OBDM with anemia, and mRNA expression of interleukin-6 and toll-like receptor (TLR) 2 was increased in OBDM group in basal high Fe and high glucose concentrations. The expression of tumor necrosis factor alpha and TLR-4 was increased in OBDM with anemia in all experimental conditions. Hepcidin mRNA expression was increased in OBDM with anemia even in basal Fe concentration, and mitofusin 2 was decreased in all experimental conditions. This study shows that obese type 2 diabetic patients have iron distribution disorders associated to their proinflammatory state, and anemic subjects have a marked elevation of hepcidin mRNA expression.

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Acknowledgments

This research was supported by grants from the Chilean Diabetes and Endocrinology Society (SOCHED) and FONDECYT with numbers 1085173 and 1110080.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Micronutrient Laboratory, Institute of Nutrition and Food Technology, University of Chile, El Líbano, 5524, Macul, Santiago, Chile

    M. Andrews & M. Arredondo

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  1. M. Andrews
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  2. M. Arredondo
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Correspondence to M. Andrews.

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Andrews, M., Arredondo, M. Ferritin Levels and Hepcidin mRNA Expression in Peripheral Mononuclear Cells from Anemic Type 2 Diabetic Patients. Biol Trace Elem Res 149, 1–4 (2012). https://doi.org/10.1007/s12011-012-9389-6

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  • DOI: https://doi.org/10.1007/s12011-012-9389-6

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