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Influence of Cadmium Exposure on Growth and Fecundity of Freshwater Mosquitofish Gambusia affinis: In Situ and In Vivo Studies

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Abstract

This study aims to investigate the effect of cadmium (Cd) exposure on growth and fecundity of mosquitofish Gambusia affinis. For this purpose, two natural populations of pregnant females of G. affinis captured from two sites were differently contaminated with Cd (S1 present Cd levels 5-fold higher than S2) and a sublethal exposure to 0.4 mg CdCl2/L (10% of LC50) during 56 days was conducted in vivo. The length–weight regression revealed a significant difference in the growth between these two populations. A significant difference in fecundity was also noted between the two populations. Indeed, the embryo numbers in pregnant females captured from S1 are significantly higher than those noted in pregnant females from S2 (21.17 ± 5 and 7.97 ± 2.12, respectively; p < 0.05). Following Cd exposure, we noted a growth perturbation resulting in lower values of both indices BWG and SGR following 7 and 21 days (−5.21 and −1.18 for BWG, and −2.09 and −0.46 for SGR, respectively) and a recuperation of growing weight at 42 and 56 days (1.32 and 1.71 for BWG, and 0.45 and 0.54 for SGR, respectively). For CF index, we observed a significant difference (p < 0.05) between control and Cd groups at 7 and 21 days of exposure, and at 21 and 56 days respectively for HSI and GSI indices. Furthermore, Cd contents in both tissues (liver and yolk sac) and fractions (cytosolic and membrane) are significantly different between groups during experimentation. In addition, the Cd contents noticed in the liver membrane fraction are significantly higher than those noted in the yolk sac tissue. The MTs levels revealed a significant difference between the control and Cd groups. In liver tissue, a significant difference was noted, in MTs levels, during the Cd exposure (7, 21, 42, and 56 days) while in the yolk sac tissue the difference was noted at 42 days of exposure. Taken together, these results imply the potential negative effect of Cd on physiological status of G. affinis as evidenced by decreasing growth and fecundity rate.

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Authors and Affiliations

  1. Laboratoire LR11ES41: Génétique, Biodiversité et Valorisation des Bioressources, Institut Supérieur de Biotechnologie, Université de Monastir, 5000, Monastir, Tunisia

    Ali Annabi, Kaouthar Kessabi, Khaled Said & Imed Messaoudi

  2. Unité de Recherche: Eléments Traces, Radicaux Libres, Antioxydants, Pathologies Humaines et Environnement, Faculté de Médecine, Université de Monastir, 5000, Monastir, Tunisia

    Abdelhamid Kerkeni

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  1. Ali Annabi
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  2. Kaouthar Kessabi
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  3. Abdelhamid Kerkeni
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  4. Khaled Said
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  5. Imed Messaoudi
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Correspondence to Ali Annabi.

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Annabi, A., Kessabi, K., Kerkeni, A. et al. Influence of Cadmium Exposure on Growth and Fecundity of Freshwater Mosquitofish Gambusia affinis: In Situ and In Vivo Studies. Biol Trace Elem Res 148, 345–355 (2012). https://doi.org/10.1007/s12011-012-9372-2

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