Abstract
This study was conducted to further explore the effects of selenium on the blood antioxidant capacity in rats exposed to fluoride to find out the optimal dosage level of selenium. Animals were divided into prevention sequence (Selenium → NaF, water → NaF) and treatment sequence (NaF → Selenium, NaF → water) (sodium fluoride 50 mg/L; sodium selenite 0.375, 0.75, 1.5 mg/L). The exposure time was 12 months. Then, the fluidity of erythrocyte membrane by electron spin resonance was analyzed, and the blood was collected for GSH-Px and SOD activity, total antioxidant capacity (T-AOC) and uric acid assay, sialic acid and MDA content. The results showed that, compared with control group, GSH-Px activity and T-AOC level increased significantly (P < 0.05), and SOD activity was raised in varying degrees in prevention and treatment groups, respectively. Uric acid level was up-regulated, but no significant differences were observed (P > 0.05). The fluidity of erythrocyte membrane showed significant increase (P < 0.05). As evident in this study, when the dose of selenium was 0.75 mg/L, all the activities of antioxidant enzymes increased significantly in prevention sequence; but in treatment sequence, the optimum intervention concentration was 1.5 mg/L. On the basis of results, the preventive effect of selenium was superior to treatment effect on the oxidative stress induced by an overdose of fluoride.
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References
Gao YH, Fu SB, Xu CB et al (2005) Action and antagonistic effects of selenium on fluorosis assciated with brick tea. Chin J Endemiol 24(1):11–13
Han B, Yoon SS, Wu PF et al (2006) Role of selenium in alteration of erythrocyte parameters in bovine fluorosis. Asian-Australas J Anim Sci 19(6):865–872
Zhu WJ, Zhang ZG, Shen XY (2009) Pathogenesis of fluorosis and the role of selenium against fluoride. Chin J Endemiol 28(6):704–706
Xu H, Zhang XY, Zhang GZ et al (2000) Alteration of free radical metabolism on fluorosis rat cerebral. Chin J Control Endem Dis 15(6):324–325
Liu XQ, Zhang XY, Li GS (2001) The role of free radical and oxidative stress in the development of bone lesion in fluorosis. Chin J Control Endem Dis 16(1):4–6
Zhu WJ, Zhang J, Zhang ZG (2011) Effects of fluoride on synaptic membrane fluidity and PSD-95 expression level in rat hippocampus. Biol Trace Elem Res 139(2):197–203
Ranjian R, Swarup D, Patra RC (2009) Oxidative stress indices in erythrocytes, liver, and kidneys of fluoride-exposed rabbits. Fluoride 42:88–93
Xu XL, Shen XY, Zhang ZG et al (2009) Combined effect of selenium and fluoride on blood antioxidant capacity of rats. J Health Toxicol 23:469–471
Feng P, Wei JR, Zhang ZG (2010) Influence of selenium and fluoride on blood antioxidant capacity of rats. Exp Toxicol Pathol. doi:10.1016/j.etp.2010.11014
Zhang LX, Zhang TF, Li LY (1997) Preparation of red cell membrane. The methods and techniques of biochemical. Higher Education Press, Bei Jing
Dodge JT, Mitchell C, Hanahem DJ (1963) The preparation and chemical characteristics of hemoglobin-free ghosts of human erythycocytes. Arch Biochem Biophys 100:119–130
Lu WY, Liu ZX, Wu R et al (2006) Electron spin resonance technique and its hurt to the red cell membrane fat. Inf Med Equip 21:4–6
Song YG, Sun J, Nohl H et al (2005) Influence of hydroxylamine derivatives and phenolic compounds on the fluidity of erythrocyte membrane. Chin J Magnetic Reson 22:350–356
Hua Y, Cheng S, Lu JW et al (1992) Study of the protection of metallothionein on myocardial cell injury hydroxyl radical. Chin Sci Bull 13:1217–1219
Guan ZZ, Yang PS (1990) Changed the level of lipid peroxidation in serum and erythrocytes and antioxidant content in rat with chronic fluorosis. Chin J Endemiol 9:4–6
Zhao Q, Wu Y, Zhang ZG et al (2011) Protective effect of selenium on fluoride-induced renal impairments in rats. Chin J Endemiol 30(2):137–141
Beck MA, Handy J, Levander OA (2004) Host nutritional status: the neglected virulence factor. Trends Microbiol 12(9):417–423
Zhang J, Wu SQ, Du S (2009) Effects of added selenium to selenium, copper, zinc and correlative antioxidase of sheep in pregnancy. Heilongjiang Anim Sci Vet Med 6:48–51
Hayshi A, Ito H, Shomori K et al (1997) Frequent occurrence of hepatocytic apoptosis in acute rejection of the grafted rat liver. Pathol Int 47(8):518
Chen BL, Li ED, Dai GJ (2005) Erythrocyte shape of patients with fluorosis scanned by electron microscope. J China-Jpn Friendship Hosp 19(4):224–226
Kanbur M, Eraslan G, Silici S et al (2009) Effects of sodium fluoride exposure on some biochemical parameters in mice: evaluation of the ameliorative effect of royal jelly applications on these parameters. Food Chem Toxicol 49:1184–1189
Shivarajashankara YM, Shivarashankara AR, Rao SH et al (2001) Oxidative stress in children with endemic skeletal fluorosis. Fluoride 34(2):103–107
Xu H, Zhao ZT, Jing L et al (2009) Study on endoplasmic reticulum stress in bone tissue of fluorosis rats. Chin J Endemiol 28(1):36–40
Tian YF, Lü J, Zhang L (2009) Study of serum sialic clinical significance in malignant tumor. Cancer Res Clin 21:756–758
Zhang M, Wang AG, He WH et al (2007) Effects of fluoride on the expression of NCAM, oxidative stress, and apoptosis in primary cultured hippocampal neurons. Toxicology 236:208–216
Kumari DS, Rao PR (1992) Red cell membrane alterations in human chronic fluoride toxicity. Fluoride 25(3):195
Guan ZZ, Wang YN, Xiao KQ et al (1998) Influence of chronic fluorosis on membrane lipids in rat brain. Neurotoxicol Teratol 20:537–542
Guan ZZ, Xiao KQ, Zeng XY et al (2000) Changed cellular membrane lipid composition and lipid peroxidation of kidney in rat with chronic fluorosis. Arch Toxicol 74:602–608
Zhang ZG, Shen XY, Xu XL (2005) Effect of combined iodine and fluoride on phospholipid and fatty acid composition of RBC in rat blood. Chin J Public Health 21:157–158
Wang ZC, Guan DH, Li XD et al (1995) Effect of free radicals on fluorosis and method of blocking. Chinese selected literary of fluoride research. Gui Yang Science and Technology Press, Gui Zhou
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This research was sponsored by the National Nature Science Foundation of China, grant number: 30871295.
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Feng, P., Wei, J. & Zhang, Z. Intervention of Selenium on Chronic Fluorosis-Induced Injury of Blood Antioxidant Capacity in Rats. Biol Trace Elem Res 144, 1024–1031 (2011). https://doi.org/10.1007/s12011-011-9087-9
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DOI: https://doi.org/10.1007/s12011-011-9087-9