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Biological and Microcalorimetric Studies of the Toxic Effect of Organoarsenic(V) Compounds to Wild Strain of Bacillus thuringiensis

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Abstract

Microcalorimetric and biological methods were carried out to determine the toxicity of dimethylarsinic acid (DMA) and monomethylarsonic acid (MMA) to wild strain of Bacillus thuringiensis. Thermokinetic parameters were obtained from the power–time curves, showing that the peak-heat output power, total heat output, and number of colonies decreased with the increases in concentration of DMA and MMA. In addition, the generation time and peak maximal time increased with the increases in the dosage of DMA and MMA. The half inhibitory concentrations of DMA and MMA were 99.02 and 142.02 μg mL−1, respectively for the wild strain of B. thuringiensis. DMA shows higher toxicity to bacteria than MMA. The toxicity resistance of B. thuringiensis against organoarsenic(V) is quite high for the wild strain. Our work demonstrates that microcalorimetry is a very sensitive, simple, and useful technique for in vitro investigation of the toxic effect of organoarsenic(V) on microbial activity.

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Acknowledgments

This work was supported in part by grants from the Sino-Italian, Sino-German PPP, Sino-Hungarian, Sino-Slovenian Governmental International Scientific and Technological Cooperation Project (200625, 20063139, 2006294, and 2007203), National Natural Science Foundation of China (40425001 and 40673065), the Specialized Research Fund for the Doctoral Program of Higher Education (20060491508), the Key Project of Chinese Ministry of Education (107077), the Hubei Key International Cooperation Project (2006CA007), and 111 project (B08030).

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Authors and Affiliations

  1. School of Environmental Studies & Key Laboratory of Biogeology and Environmental Geology of Chinese Ministry of Education & Sino-Hungarian Joint Laboratory of Environmental Science and Health, China University of Geosciences, Wuhan, 430074, People’s Republic of China

    Mohammad Russel, Jun Yao, Huilun Chen, Fei Wang, Yong Zhou & Rensheng Zhuang

  2. Department of Chemistry, Hong Kong Baptist University, 224 Waterloo Road, Kowloon Tong, Hong Kong, SAR, People’s Republic of China

    Martin M. F. Choi

  3. Institute of Ecosystem Studies, Research Unit of Pisa, National Council of Researches, Area di Ricerca, Via G. Moruzzi 1, 56124, Pisa, Italy

    Brunello Ceccanti

  4. Laboratory for Environmental Research, University of Nova Gorica, Vipavska 13, 5000, Nova Gorica, Slovenia

    Polonca Trebse

  5. Department of Chemical Technology and Environmental Chemistry, Eötvös University, 1518, Budapest, P.O. Box 32, Hungary

    Gyula Zaray

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  1. Mohammad Russel
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  2. Jun Yao
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  3. Huilun Chen
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  4. Fei Wang
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  5. Yong Zhou
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  6. Rensheng Zhuang
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  7. Martin M. F. Choi
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  8. Brunello Ceccanti
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  9. Polonca Trebse
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  10. Gyula Zaray
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Corresponding authors

Correspondence to Jun Yao or Martin M. F. Choi.

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Russel, M., Yao, J., Chen, H. et al. Biological and Microcalorimetric Studies of the Toxic Effect of Organoarsenic(V) Compounds to Wild Strain of Bacillus thuringiensis . Biol Trace Elem Res 131, 192–203 (2009). https://doi.org/10.1007/s12011-009-8358-1

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  • DOI: https://doi.org/10.1007/s12011-009-8358-1

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