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Fermentative Production of β-Carotene from Sugarcane Bagasse Hydrolysate by Rhodotorula glutinis CCT-2186

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Abstract

Β-Carotene is a red–orange pigment that serves as a precursor to important pharmaceutical molecules like vitamin A and retinol, making it highly significant in the industrial sector. Consequently, there is an ongoing quest for more sustainable production methods. In this study, glucose and xylose, two primary sugars derived from sugarcane bagasse (SCB), were utilized as substrates for β-carotene production by Rhodotorula glutinis CCT-2186. To achieve this, SCB underwent pretreatment using NaOH, involved different concentrations of total solids (TS) (10%, 15%, and 20%) to remove lignin. Each sample was enzymatically hydrolyzed using two substrate loadings (5% and 10%). The pretreated SCB with 10%, 15%, and 20% TS exhibited glucose hydrolysis yields (%wt) of 93.10%, 91.88%, and 90.77%, respectively. The resulting hydrolysate was employed for β-carotene production under batch fermentation. After 72 h of fermentation, the SCB hydrolysate yielded a β-carotene concentration of 118.56 ± 3.01 mg/L. These findings showcase the robustness of R. glutinis as a biocatalyst for converting SCB into β-carotene.

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Data available on request from the authors.

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Funding

This work was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-Brazil for scientific productivity program (Process number: 309214/2021–1) and CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior)-Brazil (Process number: 88887.514236/2020–00).

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Authors and Affiliations

  1. Department of Biotechnology, Engineering School of Lorena, University of São Paulo (EEL-USP), 12.602.810, Lorena, SP, Brazil

    Erick Díaz-Ruiz, Thércia R. Balbino, Júlio C. dos Santos, Silvio S. da Silva & Anuj K. Chandel

  2. School of Water, Energy and Environment, Cranfield University, Cranfield, MK43 0AL, UK

    Vinod Kumar

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  1. Erick Díaz-Ruiz
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  2. Thércia R. Balbino
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  3. Júlio C. dos Santos
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  5. Silvio S. da Silva
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Contributions

Erick Díaz-Ruiz completed the experiments, collected the data and contributed in manuscript writing; Thércia R. Balbino contributed in planning of the study and helped in analysis of the fermentation samples for caretenoids; Júlio C. dos Santos helped in constructive discussion on experiments and data analysis; Vinod Kumar contributed in manuscript writing and constructive discussion; Silvio S. da Silva provided some infrastructure for performance of experiments and helped in manuscript editing; Anuj K. Chandel planned and supervised the study, and provided basic infrastructure, data analysis, checked the manuscript. All authors read and approved the final manuscript.

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Correspondence to Anuj K. Chandel.

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Díaz-Ruiz, E., Balbino, T.R., dos Santos, J.C. et al. Fermentative Production of β-Carotene from Sugarcane Bagasse Hydrolysate by Rhodotorula glutinis CCT-2186. Appl Biochem Biotechnol (2023). https://doi.org/10.1007/s12010-023-04761-x

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