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High Drug Capacity of Nano-Levodopa-Liposomes: Preparation, In Vitro Release and Brain-Targeted Research

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Abstract

In this work, nano-levodopa-liposomes (L-dopa-Lip) suspension was prepared by rotary-evaporated film-ultrasonic method, and freeze-drying powders of L-dopa-Lip were also obtained to improve the stability. The products were characterized by TEM, DLS, and TG-DSC, and the phase-transition temperature (Tm) and encapsulation efficiency were calculated. The brain-targeting and in vitro release of the drug was also studied. The results showed that L-dopa-Lip were well-formed spherical vesicles, and the sizes were about 100 nm, and the encapsulation efficiency was higher than 90%. The drug release temperature of L-dopa-Lip was 68 °C, and the in vitro release property and mathematical model were also studied. The brain targeting of L-dopa-Lip in vivo was explored by injecting the gold nanoparticles (AuNPs) labeled L-dopa-Lip (AuNPs-L-dopa-Lip) through the tail vein. ICP-MS and TEM showed that L-dopa-Lip had brain targeting, suggesting the potential treatment of L-dopa-Lip on brain dysfunction. The results of this work might be helpful for designing drug-loaded liposomes for the treatment of central nervous system (CNS) diseases and monitoring their distributions in vivo.

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Data Availability

All authors confirm that the data of this article is available inside the article. The datasets generated or analyzed during this study are available from the corresponding author upon reasonable request.

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Funding

This work is completed with funding from the National Natural Science Foundation of China (31201305), the Natural Science Foundation of Hebei Province (B2019205054, B2021205001, E2022205011), the Science Foundation of Hebei Normal University (L2023J01), and the Teaching Reform project of Hebei Normal University (2021XJJG047).

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Authors and Affiliations

  1. College of Chemistry and Material Science, Hebei Key Laboratory of Organic Functional Molecules, Hebei Normal University, Shijiazhuang, 050024, China

    Shenna Chen, Lin Wang, Yue Hu, Sha Liu & Lina Geng

  2. Department of Rehabilitation Medicine, Shijiazhuang People’s Hospital, Shijiazhuang, 050000, China

    Yayong Li

Authors
  1. Shenna Chen
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  2. Lin Wang
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  3. Yue Hu
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  4. Sha Liu
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  5. Lina Geng
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  6. Yayong Li
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Contributions

All authors contributed to the study conception and design. Lina Geng and Yayong Li designed the research, material preparation, and characterization carried out by Yue Hu, and the in vivo experiments were tested by Lin Wang and Sha Liu. The first draft of the manuscript was written by Shenna Chen, and the check of the manuscript was done by Lina Geng and Yayong Li. All authors read and approved the final manuscript.

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Correspondence to Lina Geng.

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This study and included experimental procedures were approved by the Laboratory Animal Ethical and Welfare Committee (AEWC) of Hebei Normal University (IACUC-167006). All animal housing and experiments were conducted in strict accordance with the institutional guidelines for the care and use of laboratory animals.

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Chen, S., Wang, L., Hu, Y. et al. High Drug Capacity of Nano-Levodopa-Liposomes: Preparation, In Vitro Release and Brain-Targeted Research. Appl Biochem Biotechnol (2023). https://doi.org/10.1007/s12010-023-04673-w

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