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Essential Oils Infused Poly-ε-Caprolactone/Gelatin Electrospun Nanofibrous Mats: Biocompatibility and Antibacterial Study

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Abstract

Infections caused by antibiotic-resistant pathogens result in a delayed wound-healing process. As an approach to prevent infections, alternatives in the form of natural antimicrobial products have become public interest. Essential oils derived from plants are used as antimicrobials owing to their broad-spectrum activity against pathogenic organisms. In this study, essential oil from seeds of watermelon, jackfruit, and papaya was incorporated into poly-ε-caprolactone/gelatin nanofibers using an electrospinning technique. The synthesized nanofibers were smooth, continuous, and bead-free. The nanofibers were found to be mechanically competent as confirmed by the universal tensile tester. The antibacterial activity of the various essential oil-loaded nanofibrous mats was determined by disc diffusion assay. Furthermore, they were found to be non-toxic and biocompatible by MTT and CMFDA assays on fibroblast cells. The obtained results have demonstrated that essential oil-loaded nanofiber mats are promising alternatives to conventional antibacterial wound dressings.

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Data Availability

The datasets generated and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors thank the Department of Physics and Nanotechnolgy, and SCIF, SRM Institute for Science and Technology for providing the facilities to carry out this research work.

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Authors and Affiliations

  1. Department of Physics and Nanotechnology, SRM Institute of Science and Technology, Tamil Nadu, Kattankulathur, Chengalpattu, 603 203, India

    Vivekananthan Sadaiyandi & John Thiruvadigal. D

  2. Research in Environment, Sustainability Advocacy and Climate Change, Directorate of Research, SRM Institute of Science and Technology, Tamil Nadu, Kattankulathur, Chengalpattu, 603 203, India

    Raghavendra Ramalingam

  3. Faculty of Sciences, Marwadi University, Rajkot, 360003, Gujarat, India

    Kantha Deivi Arunachalam

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  1. Vivekananthan Sadaiyandi
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  2. Raghavendra Ramalingam
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  3. Kantha Deivi Arunachalam
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  4. John Thiruvadigal. D
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Conceptualization: RR, JT; methodology: RR; formal analysis and investigation: VS; writing — original draft preparation: VS; writing — review and editing: RR; resources: KDA; supervision: JT, KDA. All authors read and approved the final manuscript.

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Correspondence to Raghavendra Ramalingam or John Thiruvadigal. D.

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Sadaiyandi, V., Ramalingam, R., Arunachalam, K.D. et al. Essential Oils Infused Poly-ε-Caprolactone/Gelatin Electrospun Nanofibrous Mats: Biocompatibility and Antibacterial Study. Appl Biochem Biotechnol 196, 296–313 (2024). https://doi.org/10.1007/s12010-023-04530-w

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