Abstract
Diabetes-associated postprandial hyperglycemia is a major risk factor in cardiovascular disease. Since enzyme α-glucosidase is primarily responsible for glucose release during digestion, inhibiting it mitigates post-meal spike in blood glucose level. Metabolites from endophytic fungi could be potential natural inhibitors of this enzyme. Endophytic fungi isolated from Bauhinia purpurea L. were screened for their potential antioxidant and antidiabetic activities. Ethyl acetate extract of Nigrospora sphaerica BRN 01 (NEE) displayed high antioxidant activity with an IC50 value of 9.72 ± 0.91 µg/ml for DPPH assay and ferric reducing antioxidant power (FRAP) of 1595 ± 0.23 µmol AAE g−1 DW. NEE also showed high degree of inhibition of α-glucosidase activity with an IC50 value of 0.020 ± 0.001 mg/ml, significantly greater than the standard drug acarbose (0.494 ± 0.009 mg/ml). Metabolite profiling of NEE was carried using ultra-high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (UHPLC-ESI-QTOF-MS) and 21 metabolites identified based on the MS/MS fragmentation patterns. Docking analysis of all 21 identified metabolites was carried out. Of these, 6 showed binding energies higher than acarbose (− 6.6 kcal/mol). Based on the analysis of interactions of feruloyl glucose with active site residues of the enzyme, it could be a potential α-glucosidase inhibitor. Metabolites of Nigrospora sphaerica BRN 01, therefore, could be potential lead molecules for design and development of antidiabetic drugs.
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Acknowledgements
The authors are ever grateful to the Founder Chancellor, Sri Sathya Sai Institute of Higher Learning (SSSIHL), Bhagawan Sri Sathya Sai Baba, for constant guidance and support. The authors are thankful to SSSIHL and UGC-SAP for providing financial support to carry out this work. The authors are grateful to Central Research Instrumentation Facility (CRIF), SSSIHL, for providing all necessary facilities. The authors wish to thank V. N. Ravi Kishore Vutukuri for the help provided in optimizing mass spectrometry protocols.
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Sai Anand Kannakazhi Kantari—development of methodologies and conducting investigations; data analysis and data presentation and writing the original draft.
Ranendra Pratap Biswal—development of methodology for LC–MS analysis.
Piyush Kumar—participated in conducting investigations.
Malleswara Dharanikota—providing some resources.
Ashok Agraharam—conception or design of the work, writing (review and editing), supervision.
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Kantari, S.A.K., Biswal, R.P., Kumar, P. et al. Antioxidant and Antidiabetic Activities, and UHPLC-ESI-QTOF-MS-Based Metabolite Profiling of an Endophytic Fungus Nigrospora sphaerica BRN 01 Isolated from Bauhinia purpurea L. Appl Biochem Biotechnol 195, 7465–7482 (2023). https://doi.org/10.1007/s12010-023-04452-7
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DOI: https://doi.org/10.1007/s12010-023-04452-7