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High Concentration of Fermentable Sugars Prepared from Steam Exploded Lignocellulose in Periodic Peristalsis Integrated Fed-Batch Enzymatic Hydrolysis

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Abstract

High concentrations of fermentable sugars are a demand for economical bioethanol production. A single process strategy cannot comprehensively solve the limiting factors in high-solid enzymatic hydrolysis. The multiple intensification strategies in this study achieved the goal of preparing high-concentration fermentable sugars of corn stalk with high solid loading and low enzyme loading. First, steam explosion pretreatment enhanced the hydrophilicity of substrates and enzymatic accessibility. Second, periodic peristalsis was used to improve the mass transfer efficiency and short the liquefaction time. Additionally, fed-batch feeding and enzyme reduced the enzyme loading. Ultimately, the intensification strategies above showed that the highest fermentable sugar content was 313.8 g/L with a solids loading as much as 50% (w/w) and enzyme loading as low as 12.5 FPU/g DM. Thus, these multiple intensification strategies were promising in the high-solid enzymatic hydrolysis of steam-exploded lignocellulose.

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All data generated and analyzed during this study are included in this article.

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Funding

This study was financially supported by the National Key R&D Program of China (Grant 2019YFB1503800) and the Transformational Technologies for Clean Energy and Demonstration (Strategic Priority Research Program of the Chinese Academy of Sciences, Grant No. XDA 21060300).

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Authors and Affiliations

  1. State Key Laboratory of Biochemical Engineering, Beijing Key Laboratory of Biomass Refining Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 100190, Beijing, People’s Republic of China

    Minglu Li, Lan Wang, Qihong Zhao & Hongzhang Chen

  2. University of Chinese Academy of Sciences, 100049, Beijing, People’s Republic of China

    Minglu Li, Lan Wang, Qihong Zhao & Hongzhang Chen

Authors
  1. Minglu Li
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  2. Lan Wang
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  3. Qihong Zhao
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  4. Hongzhang Chen
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Contributions

ML: Writing - original draft, Writing - review & editing.

LW: Funding acquisition, Investigation, Project administration, Supervision, Writing - review & editing.

QZ:Methodology, Data curation, Software.

HC: Conceptualization, Investigation, Supervision, Funding acquisition.

Corresponding author

Correspondence to Lan Wang.

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Highlights

1. The highest content of fermentable sugars was 313.8 g/L.

2. Steam explosion, periodic peristalsis, fed-batch feeding and enzyme were synergistic.

3. The solid loading of hydrolysis reached as much as 50% (w/w).

4. The enzyme loading reduced as low as 12.5 FPU/g DM.

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Li, M., Wang, L., Zhao, Q. et al. High Concentration of Fermentable Sugars Prepared from Steam Exploded Lignocellulose in Periodic Peristalsis Integrated Fed-Batch Enzymatic Hydrolysis. Appl Biochem Biotechnol 194, 5255–5273 (2022). https://doi.org/10.1007/s12010-022-03969-7

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  • DOI: https://doi.org/10.1007/s12010-022-03969-7

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