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Suppression of RPL34 Inhibits Tumor Cell Proliferation and Promotes Apoptosis in Glioblastoma

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Abstract

Accumulating evidence indicates Ribosomal protein 34 (RPL34) promotes tumor malignance and its expression is associated with poor prognosis in multiple cancer cells. However, the physiological role and biological mechanism of RPL34 in glioblastoma (GBM) remain unclear. Hence, this study aimed to investigate the expression and the role of RPL34 in GBM. A total of 59 glioma samples and 12 normal brains for epilepsy surgery were used to determine the underlying mechanisms and the biological behaviors of RPL34 in GBM. In this study, we identified that RPL34 expression was significantly (p < 0.05) enriched in GBM tumors compared with low-grade glioma and normal brain, and its expression was associated with poor survival. Additionally, RPL34 was functionally required for tumor proliferation in vitro. Mechanically, inhibition of RPL34 induced glioma cell apoptosis by activation of Bad/Caspase7/PARP signaling pathway. The RPL34 promotes cell survival in GBM and could be a potential therapeutic target for GBM.

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Funding

This work was supported by the Natural Science Basic Research Plan in Shaanxi Province of China, Grant/Award (No.2019JM-498), the Clinical Research Award (No. XJTU1AF-CRF-2016–018), and the Special Foundation for “Class A Subject” of the First Affiliated Hospital of Xi’an Jiaotong University, China.

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Author notes

  1. Luoning Shi and Maode Wang contributed equally to this work.

Authors and Affiliations

  1. Department of Neurosurgery, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, 710061, Shaanxi Province, China

    Changwang Du, Jia Wang, Ping Mao, Ning Wang & Maode Wang

  2. Department of Neurosurgery, Ningxian Second People’s Hospital, Qing’Yang, 745201, Gansu Province, China

    Taoning Wang & Jinning Jia

  3. Department of Radiology, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, 710061, Shaanxi Province, China

    Junjun Li

  4. Department of Ultrasonography, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, 710061, Shaanxi Province, China

    Yi Xiao

  5. Center of Brain Science, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an , 710061, Shaanxi Province, China

    Jia Wang & Maode Wang

  6. Department of Kidney Transplantation, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, 710061, Shaanxi Province, China

    Luoning Shi

Authors
  1. Changwang Du
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  2. Taoning Wang
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  3. Jinning Jia
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  8. Ning Wang
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  9. Luoning Shi
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Contributions

CWD, LNS, and MDW contributed to the study concept and design. CWD and LNS contributed to the major research work and data collection. ZD contributed to IHC staining. MW, JJL, YX, and NW contributed to glioma samples and clinical information collection. CWD, JW, and PM contributed to data analysis. CWD wrote the manuscript. CW, HTJ, JW, and MDW contributed to critical revision. All the authors read and approved the manuscript.

Corresponding authors

Correspondence to Luoning Shi or Maode Wang.

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Du, C., Wang, T., Jia, J. et al. Suppression of RPL34 Inhibits Tumor Cell Proliferation and Promotes Apoptosis in Glioblastoma. Appl Biochem Biotechnol 194, 3494–3506 (2022). https://doi.org/10.1007/s12010-022-03857-0

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  • DOI: https://doi.org/10.1007/s12010-022-03857-0

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