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Plant Growth-Promoting Active Metabolites from Frankia spp. of Actinorhizal Casuarina spp.

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Abstract

In agriculture, plant growth enrichment via plant growth stimulating microbes has been recognized as an emergency, it is used as an alternatives to chemical pesticides and growth stimulants. The phytopathogens cause various diseases such as blister bark; stem cankers, and pink and brown rot diseases besides affect the growth frequency of Casuarina spp. toward biotic and abiotic stresses. Bio-control and plant growth-promoting potential of native Frankia isolates from Casuarina spp. in Tamil Nadu, India, was not much explored. Hence, in the present study, we are investigating the plant growth improvement activity and phytopathogen control in Casuarina spp. The Frankia sp. DDNSF-01 and Frankia casuarinae DDNSF-02 were isolated and identified from the root nodules of Casuarina spp. Additionally, it is recognized for plant growth promoter activity and in vitro antimicrobial activity against phytopathogens including Pseudomonas sp. and Colletotrichum sp. The plant growth regulators including IAA, siderophore, ammonia production, and phosphate solubilization were found out. Therefore, the formation of the most significant plant growth-promoting phytohormone IAA was confirmed by UV, FT-IR, TLC, HPLC, HPTLC, and NMR spectrum. Bioactive metabolites including methyl 4-hydroxybenzoate, dodecanoic acid, and some novel flavonoids were identified. Therefore, various growth regulators such as l-aspartic acid, 1H-indole-3-carboxaldehyde were confirmed by GC-MS spectra. The present findings conclude Frankia spp. as efficient plant growth enhancement mediator and also inhibit the phytopathogens.

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Acknowledgments

The authors thank to Bharathidasan University for the award fellowship (URF) (02492/URF/K7/2016). DST-FIST (Department of Science and Technology- Fund for improvement of S&T infrastructure), New Delhi (DST Sanction Order No.: SR/FIST/LSI-013/ 2012/Dt.13.08.2012). Thanks to Pondicherry University, Puducherry-605014, India, for providing HPLC and NMR spectrum analysis facility.

Funding

This work was supported by Research Foundation of Bharathidasan University ((URF) (02492/URF/K7/2016).

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  1. Department of Microbiology, School of Life Sciences, Bharathidasan University, Tiruchirappalli, Tamil Nadu, 620 024, India

    Narayanasamy Marappa, Lavania Ramachandran, Dhanasekaran Dharumadurai & Thajuddin Nooruddin

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  1. Narayanasamy Marappa
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The presenting author of this manuscript in ACB2019 is Narayanasamy Marappa

Session 42: YSC Agricultural & Biopharmaceutical (I), S42-07, Reference No. O02-008.

Email ID: sam16@bdu.ac.in, ORCID ID https://orcid.org/0000-0002-2812-4721

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Marappa, N., Ramachandran, L., Dharumadurai, D. et al. Plant Growth-Promoting Active Metabolites from Frankia spp. of Actinorhizal Casuarina spp.. Appl Biochem Biotechnol 191, 74–91 (2020). https://doi.org/10.1007/s12010-020-03243-8

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