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Polylactic Acid (PLA) Modified by Polyethylene Glycol (PEG) for the Immobilization of Lipase

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Abstract

This work presents a novel lipase immobilization method via polylactic acid (PLA) modified by polyethylene glycol (PEG). The immobilization performance of lipase was characterized by SEM and FTIR. The results indicated that lipase from Candida rugosa type VII was successfully immobilized on the biocompatible PLA/PEG film in the presence of 1, 6-hexamethylene diamine and glutaraldehyde. In addition, the presence of 1, 6-hexylenediamime (8%, w/w) could maintain the maximum enzymatic activity. Moreover, the optimum temperature of lipase shifted from 45 to 50 °C after immobilization. The thermal inactivation experiment illustrated that the immobilized lipase retained up to 63% of the original activity after treated at 50 °C in buffer for 120 min, significantly higher than that of the control (33%) (p < 0.05). The optimum pH value of the immobilized lipase shifted from 6.5 to 7.5. Additionally, compared with the free lipase maintaining 23% of its original activity, the immobilized lipase successfully retained up to 70% after 30 days of storage. Furthermore, the immobilized lipase displayed the excellent reusability of 82% after six cycles. In conclusion, the proposed immobilization approach developed can be potentially used as a sustainable alternative for the immobilization of lipases and the utilization of biocompatible polymer.

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Funding

This research was financially supported by the National Natural Science Foundation of China (31701526), Basic Research Fees of Universities and Colleges in Tianjin (2017KJ001), Youth Teacher Innovation Fund of Tianjin University of Science & Technology (2015LG26), the Open Project Program of State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology (No. SKLFNS-KF-201824).

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Authors and Affiliations

  1. State Key Laboratory of Food Nutrition and Safety, College of Food Engineering and Biotechnology, Tianjin University of Science & Technology, No. 29, No, 13 Ave, TEDA, Tianjin, 300457, China

    Shuhong Li, Shuang Zhao, Yajie Hou, Guiyun Chen & Ye Chen

  2. Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1, Tennodai, Tsukuba, Ibaraki, 305-8572, Japan

    Zhenya Zhang

Authors
  1. Shuhong Li
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  2. Shuang Zhao
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  3. Yajie Hou
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  4. Guiyun Chen
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  5. Ye Chen
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  6. Zhenya Zhang
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Correspondence to Ye Chen.

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Highlights

1. A novel method via polylactic acid (PLA) modified by polyethylene glycol (PEG) to immobilize lipase was employed.

2. The immobilization performance of lipase films were characterized using SEM and FTIR.

3. Factors affecting enzymatic activity of immobilized lipases such as temperature and pH were studied.

4. The thermal stability, storage stability, and reuse ability of lipases were enhanced after immobilization.

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Li, S., Zhao, S., Hou, Y. et al. Polylactic Acid (PLA) Modified by Polyethylene Glycol (PEG) for the Immobilization of Lipase. Appl Biochem Biotechnol 190, 982–996 (2020). https://doi.org/10.1007/s12010-019-03134-7

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  • DOI: https://doi.org/10.1007/s12010-019-03134-7

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