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Elucidating the Effect of Glycerol Concentration and C/N Ratio on Lipid Production Using Yarrowia lipolytica SKY7

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Abstract

The high demand for renewable energy and increased biodiesel production lead to the surplus availability of crude glycerol. Due to the above reason, the bio-based value addition of crude glycerol into various bioproducts is investigated; among them, microbial lipids are attractive. The present study was dedicated to find the optimal glycerol concentration and carbon/nitrogen (C/N) ratio to produce maximum lipid using Yarrowia lipolytica SKY7. The glycerol concentration (34.4 to168.2 g/L) and C/N ratio (25 to 150) were selected to investigate to maximize the lipid production. Initial glycerol concentration 112.5 g/L, C/N molar ratio of 100, and with 5 % v/v inoculum supplementation were found to be optimum for biomass and lipid production. Based on the above optimal parameters, lipid concentration of 43.8 % w/w with a biomass concentration of 14.8 g/L was achieved. In the case of glycerol concentration, the maximum Yp/s (0.192 g/g); Yx/s (0.43 g/g) was noted when the initial glycerol concentration was 112.5 g/L with C/N molar ratio 100 and inoculum volume 5 % v/v. The glycerol uptake was also noted to increase with the increase in glycerol concentration. At low C/N ratio, the glycerol consumption was found to be high (79.43 g/L on C/N 25) whereas the glycerol consumption was observed to decrease when the C/N ratio was raised to 150 (40.8 g/L).

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Acknowledgments

The authors would like to acknowledge the Natural Sciences and Engineering Research Council of Canada (grant A4984, Strategic grant 412994 – 11, Canada Research Chair) for financial support. We are grateful to technical staffs of INRS-ETE for their timely help to analyze the samples on GC-FID.

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Authors and Affiliations

  1. Institut National de la Recherche Scientifique, Centre Eau, Terre & Environnement, Université du Québec, 490 de la Couronne, QC, G1K 9A9, Canada

    Mathiazhakan Kuttiraja, Ayed Douha, Jose R. Valéro & Rajeswar Dayal Tyagi

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  1. Mathiazhakan Kuttiraja
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  2. Ayed Douha
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  3. Jose R. Valéro
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  4. Rajeswar Dayal Tyagi
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Correspondence to Rajeswar Dayal Tyagi.

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Kuttiraja, M., Douha, A., Valéro, J.R. et al. Elucidating the Effect of Glycerol Concentration and C/N Ratio on Lipid Production Using Yarrowia lipolytica SKY7. Appl Biochem Biotechnol 180, 1586–1600 (2016). https://doi.org/10.1007/s12010-016-2189-2

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  • DOI: https://doi.org/10.1007/s12010-016-2189-2

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