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Evaluation of Textile Dye Degradation Due to the Combined Action of Enzyme Horseradish Peroxidase and Hydrogen Peroxide

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Abstract

The kinetic parameters of the oxidant action of the combination of enzyme horseradish peroxidase (HRP) with hydrogen peroxide in the degradation of methylene blue dye were investigated. Twenty-one percent of color removal was obtained at pH 5.0 and temperature of 30 °C. Under these conditions, the kinetic parameters K m and V max of enzymatic reactions were determined for hydrogen peroxide in the absence of methylene blue dye (K m = 17.3 mM; V max = 1.97 mM/min) and in the presence of methylene blue dye (K m = 0.27 mM, V max = 0.29 μM/min). By means of analysis of phosphorescence, the presence of reactive oxygen species was detected in the form of singlet oxygen through the redox reaction between HRP and hydrogen peroxide. The existence of this reactive species is directly dependent on the concentration of hydrogen peroxide in the aqueous solution.

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Acknowledgments

We would like to thank Toyobo do Brasil for the donation of the enzyme, to CNPq (Brazilian Council for Scientific and Technological Development) for the financial support, and to David Ernest Nicodem of the Laboratory of Photochemistry of the Institute of Chemistry of UFRJ (Federal University of Rio de Janeiro) for the measurements of singlet oxygen.

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Authors and Affiliations

  1. UFRJ/EQ, Av. Athos da Silveira Ramos, 149 Bloco E, Rio de Janeiro, 21941-909, Brazil

    A. R. Pereira, L. Yokoyama & E. M. Alhadeff

  2. UERJ/IB, Rua São Francisco Xavier, 524, Rio de Janeiro, 20550-900, Brazil

    R. S. da Costa

  3. PUC-Rio, Marquês de São Vicente, 225, Rio de Janeiro, 22451-900, Brazil

    L. A. C. Teixeira

Authors
  1. A. R. Pereira
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  2. R. S. da Costa
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  3. L. Yokoyama
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  4. E. M. Alhadeff
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  5. L. A. C. Teixeira
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Correspondence to A. R. Pereira.

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Pereira, A.R., da Costa, R.S., Yokoyama, L. et al. Evaluation of Textile Dye Degradation Due to the Combined Action of Enzyme Horseradish Peroxidase and Hydrogen Peroxide. Appl Biochem Biotechnol 174, 2741–2747 (2014). https://doi.org/10.1007/s12010-014-1222-6

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  • DOI: https://doi.org/10.1007/s12010-014-1222-6

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