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A Comparative Study of Anti-Candida Activity and Phenolic Contents of the Calluses from Lythrum salicaria L. in Different Treatments

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Abstract

In the study, anti-Candida activity and phenol contents of Lythrum salicaria L. calli and wild species have been evaluated. The seeds of L. salicaria (Lythraceae), collected from Lahidjan City in the north of Iran, were cultured in Murashige and Skoog medium (MSM) with a supplement, gibberellin, to germinate. Callus inductions were performed from segments of seedling on MSM containing different concentrations of plant growth regulators, 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP). The activity of calluses extracts, wild plant, gallic acid, and 3,3′,4′-tri-O-methylellagic acid-4-O-β-d-glucopyranoside (TMEG) as the main phenolic compounds against Candida albicans was assessed using cup plate diffusion method. The total phenols contents of calli and wild plant extracts were analyzed using Folin–Ciocalteu reagent. The callus formation in MSM supplemented with various concentrations of 2,4-D and BAP were 0–100 %. Anti-Candida activity of callus extract which obtained from MSM supplemented with 2,4-D and BAP (1 mg dm−3) was similar to the wild plant extract. Minimum inhibitory concentration values of gallic acid and TMEG were obtained as 0.312 and 2.5 mg cm−3, respectively. Gallic acid equivalent values in all treatments were from 0 to 288 μg GAE mg−1. Phenolic contents of plant aerial parts (331 ± 3.7 μg GAE mg−1) and the callus, which developed in MSM including 1 mg dm−3 of both 2,4-D and BAP, showed the same phenolic value and exhibited anti-Candida extract activity.

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Abbreviations

2,4-D:

2,4-Dichlorophenoxyacetic acid

BAP:

6-Benzylaminopurine

MSM:

Murashige and Skoog Medium

GAE:

Gallic acid equivalent

TMEG:

3,3′,4′-tri-O-methylellagic acid-4-O-β-d-glucopyranoside

DMSO:

Dimethyl sulfoxide

DW:

Distilled water

TMS:

Trimethylsilane

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Authors and Affiliations

  1. Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box: 14155-6451, Tehran, 14174, Iran

    Azadeh Manayi, Samineh Jafari, Mahdi Vazirian, Tahmineh Mirnezami, Abbas Hadjiakhoondi, Mohammad Reza Shams Ardekani & Mahnaz Khanavi

  2. Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

    Soodabeh Saeidnia & Abbas Hadjiakhoondi

  3. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research Center, Tehran University of Medical Sciences, P.O. Box: 14155-6451, Tehran, 14174, Iran

    Mohammad Ali Faramarzi

  4. Department of Drug and Food Control, Faculty of Pharmacy and Pharmaceutical Quality Assurance Research Center, Tehran University of Medical Sciences, Tehran, Iran

    Nasrin Samadi

  5. Institutes of Medicinal Plants, Iranian Academic Centre for Education, Culture and Research (ACECR), P.O. Box: 33651-66171, Karaj, Iran

    Ardeshir Ghaderi

  6. Traditional Iranian Medicine and Pharmacy Research Center, Tehran University of Medical Sciences, P.O. Box: 14155-6451, Tehran, 14174, Iran

    Mohammad Reza Shams Ardekani & Mahnaz Khanavi

Authors
  1. Azadeh Manayi
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  2. Soodabeh Saeidnia
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  3. Mohammad Ali Faramarzi
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  4. Nasrin Samadi
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  5. Samineh Jafari
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  7. Ardeshir Ghaderi
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  8. Tahmineh Mirnezami
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  9. Abbas Hadjiakhoondi
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  10. Mohammad Reza Shams Ardekani
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  11. Mahnaz Khanavi
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Correspondence to Mahnaz Khanavi.

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Manayi, A., Saeidnia, S., Faramarzi, M.A. et al. A Comparative Study of Anti-Candida Activity and Phenolic Contents of the Calluses from Lythrum salicaria L. in Different Treatments. Appl Biochem Biotechnol 170, 176–184 (2013). https://doi.org/10.1007/s12010-013-0185-3

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  • DOI: https://doi.org/10.1007/s12010-013-0185-3

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