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Trehalose Has a Protective Effect on Human Brain-Type Creatine Kinase During Thermal Denaturation

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Abstract

We investigated the effects of trehalose on thermal inactivation and aggregation of human brain-type creatine kinase (hBBCK) in this study. In the presence of 1.0 M trehalose, the midpoint temperature of thermal inactivation (T m) of hBBCK increased by 4.6 °C, and the activation energy (E a) for thermal inactivation increased from 29.7 to 41.1 kJ mol−1. Intrinsic fluorescence spectra also showed an increase in the apparent transition temperature (T 1/2) of hBBCK from 43.0 °C to 46.5 °C, 47.7 °C, and 49.9 °C in 0, 0.6, 0.8, and 1.2 M trehalose, respectively. In addition, trehalose significantly blocked the aggregation of hBBCK during thermal denaturation. Our results indicate that trehalose has potential applications as a thermal stabilizer and may aid in the folding of other enzymes in addition to hBBCK.

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Abbreviations

CK:

Creatine kinase

hBBCK:

Recombinant human brain-type CK

T m :

Midpoint temperature of thermal inactivation

E a :

Activation energy

T 1/2 :

Apparent transition temperature of thermal denaturation

λ max :

Maximum emission wavelength

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Acknowledgments

This work was supported by grants of Key Science and Technology Innovation Teams of Zhejiang Province (grant nos. 2009R50031 and 2009R50031-1) from the Science and Technology Department of Zhejiang Province and the Zhejiang Provincial Top Key Discipline of Modern Microbiology and Application (grant nos. KF2010006 and KF2010007). Dr. Zi-Ping Zhang was supported by grant 30970635 of the China National Science Foundation, grant Y2101329 of the Zhejiang Provincial Science Foundation, and the grant of the Scientific and Technological Research Programs of Ning Xia Hui Autonomous Region.

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Authors and Affiliations

  1. School of Life Science, Ningxia University, Yinchuan, 750021, People’s Republic of China

    Jiang-Liu Yang & Zi-Ping Zhang

  2. School of Life Sciences, Tsinghua University, Beijing, 100084, People’s Republic of China

    Hang Mu, Zhi-Rong Lü & Hai-Meng Zhou

  3. Zhejiang Provincial Key Laboratory of Applied Enzymology, Yangtze Delta Region Institute of Tsinghua University, Jiaxing, 314006, People’s Republic of China

    Zhi-Rong Lü, Fang Zhang, Wei-Jiang Hu, Fan-Guo Meng & Hai-Meng Zhou

  4. College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, 315100, People’s Republic of China

    Shang-Jun Yin, Yue-Xiu Si & Guo-Ying Qian

  5. College of Biology and Chemical Engineering, Jiaxing University, Jiaxing, 314001, People’s Republic of China

    Sheng-Mei Zhou

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  1. Jiang-Liu Yang
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  2. Hang Mu
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Correspondence to Zi-Ping Zhang or Guo-Ying Qian.

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Jiang-Liu Yang and Hang Mu contributed equally to this study.

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Yang, JL., Mu, H., Lü, ZR. et al. Trehalose Has a Protective Effect on Human Brain-Type Creatine Kinase During Thermal Denaturation. Appl Biochem Biotechnol 165, 476–484 (2011). https://doi.org/10.1007/s12010-011-9266-3

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  • DOI: https://doi.org/10.1007/s12010-011-9266-3

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