Abstract
The medicinal fungus Ganoderma lucidum was inoculated into the media with and without supplementation of medicinal insect extracts to screen stimulators from Chinese medicinal insects for mycelial growth and triterpenoids production in submerged fermentation. The methanol and ether extracts of the tested insects had no significant stimulatory effect on the mycelial biomass production (P > 0.05), and those of H. remigator and Mylabris phalerata markedly inhibited the mycelial growth. However, the ether extract of Catharsius molossus at a concentration of 200 mg l−1 led to a significant increase in triterpenoids concentration from 231.7 ± 9.77 to 313.7 ± 10.6 mg l−1 (P < 0.01). Analysis of fermentation kinetics of G. lucidum suggests that glucose concentration in the extract of C. molossus-added group decreased more quickly as compared to the control group from day 2 to day 7 of fermentation process, while the triterpenoids biosynthesis was promoted at the same culture period. However, the culture pH profile was not affected by the addition of the extract. Chemical study of the extract show that cis-9,10-methylenehexadecanoic acid (9,10-MEA) and hexadecanoic acid (especially 9,10-MEA) were the key active compounds of the extract responsible for the stimulatory effect on the triterpenoids production.
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Acknowledgements
This work was supported by a grant (No. 30700552) from the Natural Science Foundation of China, grants (No. 09 K054 and 10 K081) from the foundation of Education Department of Hunan Province of China and a grant (No. 2009005A) from the Youth Scientific Research Foundation of Central South University of Forestry and Technology. The authors are also grateful to Du Li for helpful assistance in the measurements of extracellular triterpenoids.
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Liu, GQ., Xiao, HX., Wang, XL. et al. Stimulated Production of Triterpenoids of Ganoderma lucidum by an Ether Extract from the Medicinal Insect, Catharsius molossus, and Identification of the Key Stimulating Active Components. Appl Biochem Biotechnol 165, 87–97 (2011). https://doi.org/10.1007/s12010-011-9235-x
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DOI: https://doi.org/10.1007/s12010-011-9235-x