Opinion statement
Ovarian carcinosarcoma (OCS), also known as a malignant mixed Müllerian tumour (MMMT), is a rare and aggressive form of cancer that accounts for less than 5% of ovarian cancers. It is characterized by high morbidity and mortality rates, with a median overall survival (OS) of less than 2 years. Several factors, including advancing age, nulliparity, reduced lactation rates, decreased use of oral contraceptive pills, genetic mutations in BRCA (breast cancer) genes, and the use of assisted reproductive technology, may increase the risk of OCS. Poor prognostic factors include an advanced stage at diagnosis, older age, lymph node metastasis, suboptimal surgical cytoreduction, the presence of heterologous features on histopathology, and increased expression of vascular endothelial growth factor (VEGF), tumour protein p53, and p53 alongside Wilms tumour 1 (WT1). The main treatment approach for OCS is cytoreductive surgery followed by platinum-based chemotherapy, although immunotherapy is showing promise. Homologous recombination deficiency (HRD) testing may enhance outcomes by enabling personalized immunotherapy and targeted therapies for specific patient groups, thereby reducing unnecessary side effects and healthcare costs. However, there is currently a lack of standardised treatment regimens for OCS patients, with most studies consisting of case reports and a shortage of suitable comparator groups. This article aims to provide clinicians with information on the epidemiology, risk factors, prognostic factors, and latest therapeutic advancements in OCS.
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Introduction
Ovarian cancer ranks as the fifth primary cause of cancer-related deaths in women. According to the GLOBOCAN study, there is a projected global surge of 55% in ovarian cancer cases and a 67% rise in mortality between 2012 and 2035 [1]. The major risk factors for ovarian cancer include a family history of the disease and genetic syndromes associated with it. Although factors like obesity, smoking, and a sedentary lifestyle are linked to an increased risk of ovarian cancer, they have not been established as definitive predisposing factors [2]. Endometriosis is directly correlated with certain subtypes of ovarian cancer, specifically clear cell and endometrioid ovarian carcinoma [3]. Currently, there are no effective screening tools available, and the cost-effectiveness analyses of screening programs have yielded mixed results [4]. More than two-thirds of women are diagnosed with advanced stages of ovarian cancer, leading to estimated 5-year survival rates ranging from 20 to 40%, whilst individuals diagnosed at an early stage experience a 5-year survival rate exceeding 90% [5, 6]. The conventional treatment approach involves cytoreductive surgery combined with platinum-based chemotherapy administered before or after the operation [7]. However, as many as 80% of patients experience a relapse within 12 to 18 months after completing the treatment, requiring first-line chemotherapy based on their platinum sensitivity. Specific subsets of patients may benefit from molecular targeted therapy, which provides improved treatment responses and reduced systemic toxicity. The inherent genomic and epigenomic diversity of ovarian cancer is mirrored at the protein level, presenting potential opportunities for discovering new pharmacological targets [8].
Histologically, ovarian cancer is categorized into epithelial and non-epithelial types. Within epithelial ovarian cancers (EOC), the World Health Organisation defines seven histological subtypes—serous, endometrioid, clear cell, mesonephric-like carcinoma, dedifferentiated carcinoma, mixed carcinoma, and carcinosarcoma [9]. Each subtype represents a unique entity with variations in clinical manifestations, genetic mutations, and treatment responses.
Ovarian carcinosarcoma (OCS), otherwise known as a malignant mixed Müllerian tumour, is a rare, aggressive type of epithelial ovarian neoplasm, accounting for less than 5% of ovarian malignancies [10, 11]. Histologically, OCS consists of both high-grade carcinomatous and sarcomatous elements [10, 12]. Although there have been reports indicating similarities between OCS and more common subtypes of EOC, OCS is considered to possess distinct clinical characteristics that set it apart and result in distinctive behaviour [10, 13•, 14]. It is an uncommon form of gynaecological cancer associated with high morbidity and mortality, and the prognosis continues to be dismal [14, 15]. According to several studies, OCS is reported to have a worse prognosis compared to other forms of ovarian malignancies, such as papillary serous ovarian carcinoma, in all International Federation of Gynaecology and Obstetrics (FIGO) stages [16,17,18]. Additionally, some patients may experience non-specific symptoms of ovarian cancer (gastrointestinal disturbance, bloating, and early satiety), whilst others may be asymptomatic, further contributing to poor survival via late clinical presentation and consequently a more advanced FIGO stage at diagnosis [19]. Whilst more common types of EOC are managed with cytoreductive surgery and neo- and/or adjuvant platinum-based chemotherapy, OCS has no established or standardised treatments, and large-scale prospective studies remain scarce [20, 21].
This article aims to provide clinicians with information regarding the epidemiology of OCS, risk factors associated with the disease, prognostic factors, and the evaluation of the latest developments in therapeutics.
Epidemiology and risk factors
Globally, ovarian cancer is the fifth most common gynaecological cancer, accounting for approximately a quarter of a million diagnoses and more than 150,000 deaths each year [2, 22]. Higher incidence rates and mortality risks have been identified in more developed regions [2]. EOC is the most predominant type of ovarian cancer, whereas OCS represents a rare yet biologically unique ovarian cancer with a poor prognosis [23, 24]. According to a consensus review conducted by the Gynecologic Cancer InterGroup, the median overall survival (OS) for OCS is reported to be less than 2 years, and approximately 90% of OCS cases exhibit malignant spread [24].
In the UK, on average, more than a quarter of new ovarian cancer cases (28%) are diagnosed in patients aged 75 and over [25]. Similarly to most cancer types, incidence increases with age, as this largely reflects cell DNA damage accumulating over time. The average age of OCS onset is between 60 and 70 years of age [26]. Ovarian cancer incidence rates are projected to rise by 5% in the UK over the next two decades, which could be due to population growth and an ageing population, but also other risk factors such as increasing nulliparity rates and decreased lactation, decreasing use of oral contraceptive pills, and increasing use of assisted reproductive technology and mutations in BRCA genes [25, 27].
Detailed regional statistics for OCS incidence or distribution are unavailable due to their rarity, thus providing difficulty for critical analysis of regional differences [10, 11]. Racial disparities among 2866 patients with OCS were investigated by the Uniformed Services University, USA, and it was found that race was not an independent prognostic factor in OCS [28]. Patients’ characteristics must be viewed holistically in appreciation of socioeconomic and comorbid status, which may be more pronounced among different demographics. Nevertheless, ovarian cancer in England was not found to be associated with deprivation, although it is important to consider the variations in healthcare systems internationally, as the National Health Service (NHS) “free at the point of use” ethos is not universally applicable and financial cost could contribute to late presentation.
Prognostic factors
One important factor associated with worse survival in patients with OCS is the advanced stage of the disease, according to the FIGO classification [13•, 29, 30]. A study reviewed 37 cases of OCS, with 70% of patients staged at III or IV, and reported a significant decrease in survival rates, dropping from 40 to 6% after a 5-year period [30]. It concluded that the early FIGO stage was an independent prognostic factor for OCS survival. A 2011 study discovered that patients with advanced OCS received poorer response rates to platinum-based chemotherapy compared to the cohort of patients with serous EOC [31], which is supported by other studies that noticed consistently worse survival in all stages of OCS compared with individuals diagnosed with high-grade serous ovarian cancer [10, 17]. Older age at diagnosis has also been identified as a poor prognostic factor in patients with OCS, alongside the presence of lymph node metastasis [29, 31, 32]. A 2010 study reported significantly improved survival in patients who underwent lymphadenectomy, reducing the mortality risk by 34% in such patients compared with those who did not have lymphadenectomy [29]. Additionally, the presence of residual disease following debulking surgery is linked to reduced survival rates and lower disease-free survival, mainly due to an increased risk of recurrence. Therefore, achieving optimal surgical cytoreduction is a crucial prognostic factor that significantly impacts survival outcomes [33,34,35].
Histopathologic features may represent an additional predictor of prognosis. Various studies have supported the hypothesis that heterologous features—elements that are not usually present in the ovary—are correlated with less favourable outcomes [34,35,36,37], whilst others have contradicted these findings [30, 33, 38, 39]. One study investigated the effects of epithelial and stromal tumour components on advanced OCS and concluded that tumours with more than 25% stromal components significantly resulted in worse outcomes [39]. Increased expression of vascular endothelial growth factor (VEGF), a potent angiogenic factor, and an increased number of small vessels are important in predicting poor survival [40]. High expression of the mutated form of tumour suppressor protein p53 has been frequently observed in gynaecological malignancies, particularly in OCS [41, 42]. Overexpression of p53 alongside Wilms tumour 1 (WT1) protein resulted in reduced OS [43]. Carter et al. discovered that the 20-year survival probability of individuals with no expression of either p53 or WT1 was 67.7%, which drastically reduced to 6.5% in those with both WT1 and p53 expression [43]. Whilst there is insufficient data on the influence of the Ki67 antigen expression on OCS survival, one study concluded that the 5-year survival in the highly-expressed Ki67 group was 15.9% compared to 36.4% in the low-expressed group but was deemed statistically insignificant [38]. There are various studies investigating the expression of Ki67 in other gynaecological malignancies, such as uterine carcinosarcomas [44,45,46,47]. Finally, cancer antigen 125 (CA 125) levels were found to be elevated in 74% of OCS patients but deemed to be statistically insignificant for survival prediction [14]. However, a study conducted by Nazari et al. reported a statistically significant relationship between CA 125 levels and OCS staging, although the authors acknowledged its limitations as a sole prognostic tool [48].
Therapeutic developments
Chemotherapy
Paclitaxel and carboplatin (PC) have been widely used as combined chemotherapy in the management of EOC [49], but the most effective regimen for OCS is not yet known. For the treatment of OCS, a 2022 randomised controlled trial (RCT) investigated the efficacy of PC compared to paclitaxel and ifosfamide; longer OS (30 vs. 25 months) and progression-free survival (PFS) (15 vs. 10 months) were reported in the PC group [50]. However, this trial explored both uterine carcinosarcoma (UCS) and OCS, enrolling a large group of 449 eligible patients with UCS but only 90 eligible OCS patients. The results regarding OCS specifically were reported to be determined with limited precision and found to be statistically insignificant. A phase II RCT (MITO-26) recruited 45 patients, with advanced or recurrent OCS (n = 32) and UCS (n = 13), to assess the safety and efficacy of trabectedin across seven centres in Italy [51]. To be eligible for the study, patients needed to have a disease that was not controllable through surgery and radiotherapy. Additionally, they were required to have received a maximum of two lines of chemotherapy prior to the commencement of the study. Regarding adverse events, 8 patients experienced decreased white cell and neutrophil counts, 6 patients had increased alanine aminotransferase (ALT) and aspartate aminotransferase (AST), and 2 patients died directly as a result of drug-related haematological toxicity. The objective response rate (ORR) was 11.9%, with a median PFS and OS of 2.01 and 4.64 months, respectively. However, the MITO-26 study acknowledges that, due to the current absence of a standardised treatment, a comparator treatment cannot be utilised to assess the drug’s efficacy. Furthermore, the study did not measure the primary endpoint for OCS patients exclusively, and therefore, trabectedin as monotherapy in OCS management needs further exploration.
A retrospective cohort study explored patients who were experiencing persistent or recurrent gynaecological carcinosarcomas and investigated the impact of second-line chemotherapy on OS in both UCS and OCS [52]. Among 46 patients, only 7 were confirmed OCS patients who received second-line chemotherapy regimens of adriamycin (n = 1), carboplatin and docetaxel (n = 4), PC (n = 1), and other regimens (n = 1). The decision to receive monotherapy or combined chemotherapy was determined by whether the patient had a treatment interval of less or greater than 180 days. PFS and median OS were calculated as 6.9 and 11.9 months respectively, but the results were not statistically significant. The study had an inadequate sample size to confirm whether combination or monotherapy was more efficient than the other, and further work is needed to establish first- and second-line therapies for OCS.
Surgery and Chemotherapy
Optimal cytoreductive surgery followed by chemotherapy has been frequently considered in the primary management of the disease, despite no available RCTs [53]. A retrospective study reviewed a cohort of patients (n = 267) from 1999 to 2021 who were diagnosed with peritoneal dissemination from tubo-ovarian malignancies and had undergone cytoreductive surgery (CRS) and hyperthermic intraperitoneal chemotherapy (HIPEC) [54]. Sixteen out of the 267 patients had confirmed OCS with advanced disease (FIGO stage III or above) and received adjuvant chemotherapy with either cisplatin, a combination of cisplatin and doxorubicin, or melphalan. Management with CRS and HIPEC reported no 90-day mortality and was associated with low rates of malignant bowel obstruction; nevertheless, its significance on the median OS cannot be determined until larger cohort studies with longer follow-ups are performed. Interestingly, another retrospective study conducted by Nizam et al. identified 27 OCS patients and investigated the impact of adjuvant platinum-based chemotherapy on OS [53]. The study concluded that the OS in patients who underwent adjuvant chemotherapy was significantly improved compared to those who did not, and the stage of disease did not affect the outcome. Further support came from a cohort study conducted by Hollis et al. in 2022, which revealed an extended OS among OCS patients who underwent debulking surgery followed by platinum-based adjuvant chemotherapy [10]. However, the investigators concluded that residual disease absence after debulking surgery and early-stage disease were both strongly associated with improved survival [10].
A case series conducted by Sethi et al. studied patients receiving adjuvant chemotherapy with PC after a total abdominal hysterectomy (TAH) with bilateral salpingo-oophorectomy (BSO) and complete lymph node dissection (LND) [55]. The paper concluded that cytoreductive surgery with LND observed better survival compared to minimal surgery. Similarly, another patient underwent a TAH, bilateral adnexectomy, omentectomy, and lymphadenectomy with no residual disease, followed by PC treatment [56]. The published report noted that the patient was disease-free when followed up at 45 months. Additionally, a large French retrospective multi-centre study included 425 patients with gynaecological carcinosarcomas, of whom 112 women had advanced OCS (FIGO stage III or above) [57]. Analysis revealed that OCS patients who underwent both upfront primary debulking and interval cytoreductive surgery followed by front-line chemotherapy had significantly better PFS.
Kozłowski et al. explored 14 patients with primary OCS. Among them, 10 patients underwent surgery and adjuvant platinum-based chemotherapy [58]. The authors reported one particular patient who had a recurrence 29 months after diagnosis, after initially being treated with platinum and paclitaxel chemotherapy prior to surgery, subsequently received adjuvant chemotherapy with the same regimens, and had an OS of 46 months. However, the results of other patients were not discussed, and the impact of surgery and chemotherapy or surgery alone on the OS could not be determined. Interestingly, Heinzelmann-Schwarz et al. conducted a retrospective study looking at the efficacy of adjuvant chemotherapy in various subtypes of endometrial and ovarian cancer, including 17 OCS patients [59]. The study found that OCS had a higher chemotherapy sensitivity (73.9%) to a combination of carboplatin and anthracyclines than carboplatin/taxane-based (39.5%) and carboplatin/alkylating combinations (24.2%), although statistical significance was not discussed. Further studies to confirm the value of carboplatin/anthracyclines as the optimal adjuvant chemotherapy must be performed. A case report mentioned one particular patient with advanced OCS who underwent neoadjuvant chemotherapy (NACT), followed by non-curative unilateral resection of the appendicular organ, total omentectomy, and adjuvant chemotherapy [60]. She experienced recurrence roughly 21 months after this initial surgery and received aggressive tumour resection of the para-aortic and/or pelvic tumours, and replacement of the abdominal aorta and/or iliac arteries with a synthetic arterial graft. Unfortunately, there were confirmed lung and mediastinal lymph node metastases 1.3 years later, and the patient died 5.9 years after diagnosis. It is interesting to note that this patient had a greater OS compared to other studies reviewed, suggesting the benefits of radical surgical intervention in OCS.
Zuhdy et al. reviewed three cases of OCS associated with axillary lymph node metastasis who either received NACT or adjuvant therapy, but the median OS and efficacy of individual treatment approaches could not be determined due to patients being lost to follow-up [61]. To decide on the best suitable treatment for OCS with axillary lymph node metastasis, multi-centre studies with large sample sizes are required.
Surgery, Chemotherapy, and Targeted Therapy
A recently published report described a patient diagnosed with rapidly progressive OCS who had undergone uncomplicated embryo transfer 16 days prior [62]. The patient received 4 cycles of NACT with PC every 3 weeks. Interval computed tomography (CT) imaging demonstrated reduced peritoneal disease and resolved ascites, and subsequently, the patient underwent optimal debulking surgery. The patient received 2 more cycles of adjuvant platinum-based chemotherapy with the addition of bevacizumab, which was continued as maintenance therapy. At the time of writing, the most recent CT scan showed no evidence of disease, but this report does raise the question of the long-term effects of assisted reproduction technology on developing OCS. Nevertheless, NACT may have an important role in optimising debulking surgery in OCS, thus improving patient outcomes, which has also been supported by Liu et al. [63]. A case report discussed a 61-year-old female with advanced OCS who underwent extensive cytoreductive surgery including a hysterectomy, bilateral adnexectomy, partial resection of the rectum and sigmoid colon, and LND [21]. She subsequently received 6 cycles of chemotherapy with PC, plus bevacizumab during cycles 3 to 6. HRD testing was positive, so it was decided to put the patient on bevacizumab and the PARP inhibitor niraparib, but the patient developed severe myelosuppression, and thus, she received oral niraparib as maintenance therapy instead. Six months after the sixth cycle of chemotherapy, the CA 125 levels dropped from 263.4 to 4.55 U/ml, reducing to normal levels. Long-term survival data were not reported. However, during the six-month follow-up, imaging assessments revealed no signs of tumour recurrence or disease progression.
Ovarian teratoid carcinosarcoma (OTC) is an even rarer type of OCS, with the addition of an immature neuroectodermal component [64, 65]. Two case reports investigated two women with confirmed OTC [64, 65]. A 60-year-old female was diagnosed with stage IC3 OTC and underwent radical TAH-BSO, pelvic and para-aortic lymphadenectomy, and subtotal omentectomy [64]. Two months post-surgery, the patient experienced recurrent disease with multiple liver and bone metastases on positron emission tomography CT scan (PET-CT). However, following 4 cycles of vincristine, actinomycin, and cyclophosphamide chemotherapy, a complete response was achieved. Subsequently, 2 months after completing chemotherapy, the patient experienced recurrent peritoneal dissemination. The treatment involved 6 cycles of PC combined with bevacizumab, resulting in a partial response [64]. Unfortunately, 2 weeks after completing chemotherapy, the patient died due to complications from bevacizumab. Similarly, Fox et al. reported a 55-year-old female who was diagnosed with stage IC OTC and underwent optimal cytoreductive surgery with TAH-BSO, pelvic lymphadenectomy, and omentectomy [65]. The patient recurred 1 month after the surgery and was then given 6 cycles of ifosfamide and paclitaxel, followed by 3 cycles of carboplatin, gemcitabine, and bevacizumab. Chemotherapy was discontinued after a follow-up CT scan confirmed disease progression; therefore, the patient received 2 cycles of nivolumab, which was not effective. Due to disease progression, the patient was given a 5-day cycle of bleomycin, etoposide, and cisplatin. However, 1 week later, the patient died due to neutropenic sepsis, prior to receiving cycle two. The OS was 14 months since the initial surgery. Both patients experienced recurrent disease 1 to 2 months after surgery, confirming the aggressive nature of the disease. Furthermore, a PIK3CA mutation was detected in both patients, suggesting that targeting the PIK3CA pathway may be valuable in the treatment of OTC.
Future directions
Table 1 summarizes ongoing investigations for the management of OCS. The emphasis of current trials is mostly centred on establishing the role of targeted therapies [13•]. Indeed, current clinical trials encompass a range of treatment approaches, either as singular interventions or in combination. These strategies involve PD-1 [66,67,68,69,70,71,72], PD-L1 [73], VEGF [71, 73, 74], PARP [72, 73], cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) [69], mTOR [75], or complement component 3 (C3) [74] inhibitors.
The majority of ongoing clinical trials involve PD-1/PD-L1 inhibitors. PD-1/PD-L1 is a receptor-ligand system and can prevent anti-tumour immune responses [76,77,78]. T cells of the immune system greatly express PD-1, whereas cancer cells and antigen-presenting cells express PD-L1 [78, 79]. Additionally, PD-L1 is highly expressed in ovarian cancer, and it is associated with tumour-infiltrating lymphocytes, cancer stem cell populations expressing CD44 (a cell-surface glycoprotein contributing to metastasis), and other stem cell markers [80]. Inhibiting PD-L1 via immune checkpoint inhibitors, such as durvalumab, may downregulate stem cell populations associated with cancer recurrence [80]. The efficacy of PD-1/PD-L1 inhibitors underwent a systematic review and meta-analysis by Chen et al., based on 91 phase I–III clinical trials of various cancers, including those of gynaecological origin [81]. This meta-analysis demonstrated that PD-1/PD-L1 inhibitors in combination with chemotherapy had a statistically significantly higher ORR when compared to immunotherapy alone.
As promising as this emerging therapy may appear, data regarding the effectiveness of immunotherapy in OCS are limited. The JAVELIN Ovarian 100 study of 998 patients—including an unspecified number of OCS patients—evaluated the PD-L1 inhibitor avelumab as a combination therapy versus maintenance therapy after PC chemotherapy [82]. The trial was halted prematurely as the interim analysis revealed that the addition of the checkpoint inhibitor avelumab to chemotherapy did not result in an improvement in PFS. The JAVELIN Ovarian 200 study enrolled a total of 566 patients—including 6 patients with OCS—who had platinum-resistant disease and had undergone no more than 3 previous therapies [83]. These patients were randomised into different groups receiving avelumab, pegylated liposomal doxorubicin (PLD), or a combination of treatments. The limited number of OCS patients in the sample size prevented the evaluation of response measurements for this specific population. Regarding ovarian cancer as a whole, the results indicated that neither avelumab alone nor avelumab in combination with PLD showed improvements in PFS or OS compared to PLD alone. This may suggest that the combination of chemotherapy and immunotherapy might not be the most effective treatment approach for OCS patients. Patient selection could be improved through the analysis of tumour histology, HRD status, and previous treatments [84, 85]. There is currently an inadequacy of large-scale studies evaluating HRD rates among OCS patients. A recent study demonstrated that HRD testing results can be obtained in a timely manner for making therapeutic decisions by ensuring maximal tumour tissue is collected during the initial diagnosis, thereby ensuring optimal tumour quality and quantity for testing [84]. To enhance accuracy, it was observed that conducting additional testing for BRCA mutations reduces the occurrence of HRD testing failures and should be routinely performed.
Immunotherapy has played a pivotal role in enhancing the outcome of several malignancies, including non-small cell lung cancer, melanoma, bladder cancer, and kidney cancer [86,87,88,89,90]. Nevertheless, the application of targeted therapies for OCS is primarily supported by preclinical data, as findings from clinical trials have been disappointing [1]. The ultimate objective is to achieve personalized immunotherapy, enabling tailored treatments for different OCS patients with distinct characteristics, thus avoiding needless side effects and minimizing healthcare expenses [1].
Conclusion
OCS is a rare and aggressive malignancy, and its aetiology remains poorly understood. The rarity of the disease poses challenges in identifying the most effective therapeutic strategies to enhance patient outcomes. Given its unique histology and poorer survival rates compared to EOC, OCS may require a distinct approach. However, the lack of sufficient studies makes it difficult to establish a standardised treatment plan for OCS patients, as most existing research primarily consists of case studies involving various treatment regimens without appropriate comparator treatment groups. The best available evidence continues to support cytoreductive surgery, followed by platinum-based chemotherapy. Despite the promising potential of the PD-1/PD-L1 receptor-ligand system as an immuno-oncology target, there is conflicting evidence concerning the effectiveness of immune checkpoint inhibitors in OCS. This disparity highlights the necessity for more rigorous studies to gain a comprehensive understanding of their role in treating the OCS. HRD testing may identify specific patient populations for targeted therapies. Given the heterologous nature of OCS, maximal tumour tissue should be obtained during the initial intervention to achieve optimal tumour quality and quantity for HRD testing.
Data availability
Not applicable.
References and Recommended Reading
Papers of particular interest, published recently, have been highlighted as: • Of importance
Borella F, Ghisoni E, Giannone G, Cosma S, Benedetto C, Valabrega G, et al. Immune checkpoint inhibitors in epithelial ovarian cancer: an overview on efficacy and future perspectives. Diagnostics (Basel). 2020;10:146.
Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A, et al. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2021;71:209–49.
Momenimovahed Z, Tiznobaik A, Taheri S, Salehiniya H. Ovarian cancer in the world: epidemiology and risk factors. Int J Womens Health. 2019;11:287–99.
Samartzis EP, Labidi-Galy SI, Moschetta M, Uccello M, Kalaitzopoulos DR, Perez-Fidalgo JA, et al. Endometriosis-associated ovarian carcinomas: insights into pathogenesis, diagnostics, and therapeutic targets-a narrative review. Ann Transl Med. 2020;8:1712.
Ghose A, Bolina A, Mahajan I, Raza SA, Clarke M, Pal A, et al. Hereditary ovarian cancer: towards a cost-effective prevention strategy. Int J Environ Res Public Health. 2022;19:12057.
Jones MR, Kamara D, Karlan BY, Pharoah PDP, Gayther SA. Genetic epidemiology of ovarian cancer and prospects for polygenic risk prediction. Gynecol Oncol. 2017;147:705–13.
Berek JS, Renz M, Kehoe S, Kumar L, Friedlander M. Cancer of the ovary, fallopian tube, and peritoneum: 2021 update. Int J Gynecol Obstet. 2021;155(Suppl. 1):61–85. https://doi.org/10.1002/ijgo.13878.
Moufarrij S, Dandapani M, Arthofer E, Gomez S, Srivastava A, Lopez-Acevedo M, et al. Epigenetic therapy for ovarian cancer: promise and progress. Clin Epigenetics. 2019;11:7. https://doi.org/10.1186/s13148-018-0602-0.
McCluggage WG, Singh N, Gilks CB. Key changes to the World Health Organization (WHO) classification of female genital tumours introduced in the 5th edition (2020). Histopathology. 2022;80(5):762–78.
Hollis RL, Croy I, Churchman M, Bartos C, Rye T, Gourley C, et al. Ovarian carcinosarcoma is a distinct form of ovarian cancer with poorer survival compared to tubo-ovarian high-grade serous carcinoma. Br J Cancer. 2022;127:1034–42.
del Carmen MG, Birrer M, Schorge JO. Carcinosarcoma of the ovary: a review of the literature. Gynecol Oncol. 2012;125:271–7.
Jin Z, Ogata S, Tamura G, Katayama Y, Fukase M, Yajima M, et al. Carcinosarcomas (malignant mullerian mixed tumors) of the uterus and ovary: a genetic study with special reference to histogenesis. Int J Gynecol Pathol. 2003;22:368–73.
• Boussios S, Karathanasi A, Zakynthinakis-Kyriakou N, Tsiouris AK, Chatziantoniou AA, Kanellos FS, et al. Ovarian carcinosarcoma: current developments and future perspectives. Crit Rev Oncol Hematol. 2019;134:46–55. Review on the epidemiology, pathology, prognostic factors, clinical presentation, and therapeutic interventions in the field of ovarian carcinosarcoma.
Brown E, Stewart M, Rye T, Al-Nafussi A, Williams AR, Bradburn M, et al. Carcinosarcoma of the ovary: 19 years of prospective data from a single center. Cancer. 2004;100:2148–53.
Paulsson G, Andersson S, Sorbe B. A population-based series of ovarian carcinosarcomas with long-term follow-up. Anticancer Res. 2013;33:1003–8.
Brackmann M, Stasenko M, Uppal S, Erba J, Reynolds RK, McLean K. Comparison of first-line chemotherapy regimens for ovarian carcinosarcoma: a single institution case series and review of the literature. BMC Cancer. 2018;18:172.
Rauh-Hain JA, Gonzalez R, Bregar AJ, Clemmer J, Hernández-Blanquisett A, Clark RM, et al. Patterns of care, predictors and outcomes of chemotherapy for ovarian carcinosarcoma: A National Cancer Database analysis. Gynecol Oncol. 2016;142:38–43.
Rauh-Hain JA, Diver EJ, Clemmer JT, Bradford LS, Clark RM, Growdon WB, et al. Carcinosarcoma of the ovary compared to papillary serous ovarian carcinoma: a SEER analysis. Gynecol Oncol. 2013;131:46–51.
Dilley J, Burnell M, Gentry-Maharaj A, Ryan A, Neophytou C, Apostolidou S, et al. Ovarian cancer symptoms, routes to diagnosis and survival - population cohort study in the ‘no screen’ arm of the UK Collaborative Trial of Ovarian Cancer Screening (UKCTOCS). Gynecol Oncol. 2020;158:316–22.
Vanderpuye VD, Clemenceau JRV, Temin S, Aziz Z, Burke WM, Cevallos NL, et al. Assessment of adult women with ovarian masses and treatment of epithelial ovarian cancer: ASCO resource-stratified guideline. JCO Glob Oncol. 2021;7:1032–66.
Fu J. Management of a rare ovarian carcinosarcoma: a case report and literature review. Exp Ther Med. 2022;24:583.
Huang J, Chan WC, Ngai CH, Lok V, Zhang L, Lucero-Prisno DE 3rd, et al. Worldwide burden, risk factors, and temporal trends of ovarian cancer: a global study. Cancers (Basel). 2022;14:2230.
Reid BM, Permuth JB, Sellers TA. Epidemiology of ovarian cancer: a review. Cancer Biol Med. 2017;14:9–32.
Berton-Rigaud D, Devouassoux-Shisheboran M, Ledermann JA, Leitao MM, Powell MA, Poveda A, et al. Gynecologic Cancer InterGroup (GCIG) consensus review for uterine and ovarian carcinosarcoma. Int J Gynecol Cancer. 2014;24:S55-60.
Cancer Research UK. Ovarian cancer statistics. 2015. https://www.cancerresearchuk.org/health-professional/cancer-statistics/statistics-by-cancer-type/ovarian-cancer Accessed 01 Jul 2023.
Makris GM, Siristatidis C, Battista MJ, Chrelias C. Ovarian carcinosarcoma: a case report, diagnosis, treatment and literature review. Hippokratia. 2015;19(3):256–9.
Flaum N, Crosbie EJ, Edmondson RJ, Smith MJ, Evans DG. Epithelial ovarian cancer risk: a review of the current genetic landscape. Clin Genet. 2020;97:54–63.
Rojas C, Tian C, Powell MA, Chan JK, Bateman NW, Conrads TP, et al. Racial disparities in uterine and ovarian carcinosarcoma: a population-based analysis of treatment and survival. Gynecol Oncol. 2020;157:67–77.
Garg G, Shah JP, Kumar S, Bryant CS, Munkarah A, Morris RT. Ovarian and uterine carcinosarcomas: a comparative analysis of prognostic variables and survival outcomes. Int J Gynecol Cancer. 2010;20:888–94.
Chang J, Sharpe JC, A’Hern RP, Fisher C, Blake P, Shepherd J, et al. Carcinosarcoma of the ovary: incidence, prognosis, treatment and survival of patients. Ann Oncol. 1995;6:755–8.
Rauh-Hain JA, Growdon WB, Rodriguez N, Goodman AK, Boruta DM 2nd, Schorge JO, et al. Carcinosarcoma of the ovary: a case-control study. Gynecol Oncol. 2011;121:477–81.
Wang WP, Li N, Zhang YY, Gao YT, Sun YC, Ge L, et al. Prognostic significance of lymph node metastasis and lymphadenectomy in early-stage ovarian carcinosarcoma. Cancer Manag Res. 2018;10:1959–68.
Harris MA, Delap LM, Sengupta PS, Wilkinson PM, Welch RS, Swindell R, et al. Carcinosarcoma of the ovary. Br J Cancer. 2003;88:654–7.
Sood AK, Sorosky JI, Gelder MS, Buller RE, Anderson B, Wilkinson EJ, et al. Primary ovarian sarcoma: analysis of prognostic variables and the role of surgical cytoreduction. Cancer. 1998;82:1731–7.
Kim HJ, Lee HM, Kim MK, Lee YK, Lee IH, Lee KH, et al. Prognostic assessment of sarcomatous histologic subtypes of ovarian carcinosarcoma. Obstet Gynecol Sci. 2017;60:350–6.
Dehner LP, Norris HJ, Taylor HB. Carcinosarcomas and mixed mesodermal tumors of the ovary. Cancer. 1971;27:207–16.
Dictor M. Malignant mixed mesodermal tumor of the ovary: a report of 22 cases. Obstet Gynecol. 1985;65:720–4.
Ariyoshi K, Kawauchi S, Kaku T, Nakano H, Tsuneyoshi M. Prognostic factors in ovarian carcinosarcoma: a clinicopathological and immunohistochemical analysis of 23 cases. Histopathology. 2000;37:427–36.
Athavale R, Thomakos N, Godfrey K, Kew F, Cross P, de Barros LA, et al. The effect of epithelial and stromal tumor components on FIGO stages III and IV ovarian carcinosarcomas treated with primary surgery and chemotherapy. Int J Gynecol Cancer. 2007;17:1025–30.
Näyhä V, Stenbäck F. Angiogenesis and expression of angiogenic agents in uterine and ovarian carcinosarcomas. APMIS. 2008;116:107–17.
Berchuck A, Elbendary A, Havrilesky L, Rodriguez GC, Bast RC Jr. Pathogenesis of ovarian cancers. J Soc Gynecol Investig. 1994;1:181–90.
Liu FS, Ho ES, Chen JT, Shih RT, Yang CH, Shih A. Overexpression or mutation of the p53 tumor suppressor gene does not occur in malignant ovarian germ cell tumors. Cancer. 1995;76:291–5.
Carter JH, Deddens JA, Mueller G, Lewis TG, Dooley MK, Robillard MC, et al. Transcription factors WT1 and p53 combined: a prognostic biomarker in ovarian cancer. Br J Cancer. 2018;119:462–70.
Swisher EM, Gown AM, Skelly M, Ek M, Tamimi HK, Cain JM, et al. The expression of epidermal growth factor receptor, HER-2/Neu, p53, and Ki-67 antigen in uterine malignant mixed mesodermal tumors and adenosarcoma. Gynecol Oncol. 1996;60:81–8.
Iwasa Y, Haga H, Konishi I, Kobashi Y, Higuchi K, Katsuyama E, et al. Prognostic factors in uterine carcinosarcoma: a clinicopathologic study of 25 patients. Cancer. 1998;82(3):512–9.
Kitson S, Sivalingam VN, Bolton J, McVey R, Nickkho-Amiry M, Powell ME, et al. Ki-67 in endometrial cancer: scoring optimization and prognostic relevance for window studies. Mod Pathol. 2017;30:459–68.
Zhou Y, Jiang HG, Lu N, Lu BH, Chen ZH. Expression of ki67 in papillary thyroid microcarcinoma and its clinical significance. Asian Pac J Cancer Prev. 2015;16:1605–8.
Nazari Z, Rastegar A, Nasouhi M, Mostafavi EB, Montazer F. The evaluation of serum levels of erythrocyte sedimentation rate and C-reactive protein with CA125 as probable predictive factors of malignancy in adnexal masses. J Young Pharm. 2019;11:304–8.
Kim A, Ueda Y, Naka T, Enomoto T. Therapeutic strategies in epithelial ovarian cancer. J Exp Clin Cancer Res. 2012;31:14.
Powell MA, Filiaci VL, Hensley ML, Huang HQ, Moore KN, Tewari KS, et al. Randomized phase III trial of paclitaxel and carboplatin versus paclitaxel and ifosfamide in patients with carcinosarcoma of the uterus or ovary: an NRG oncology trial. J Clin Oncol. 2022;40:968–77.
Lorusso D, Pignata S, Tamberi S, Mangili G, Bologna A, Nicoloso MS, et al. Efficacy and safety of trabectedin for the treatment of advanced uterine or ovarian carcinosarcoma: results of a phase II multicenter clinical trial (MITO-26). Gynecol Oncol. 2022;167:436–43.
Ebata T, Yonemori K, Nishikawa T, Sudo K, Shimomura A, Noguchi E, et al. Treatment outcome of second-line chemotherapy for gynecologic carcinosarcoma. Oncology. 2020;98:699–705.
Nizam A, Bustamante B, Shan W, Shih KK, Whyte JS, Sakaris A, et al. Overall survival and adjuvant therapy in women with ovarian carcinosarcoma: a single-institution experience. Diagnostics (Basel). 2019;9:200.
Lopez-Ramirez F, Sardi A, Studeman K, King MC, Falla-Zuniga LF, Nikiforchin A, et al. Outcomes after cytoreductive surgery and hyperthermic intraperitoneal chemotherapy for peritoneal dissemination from ovarian carcinosarcoma. Eur J Surg Oncol. 2023;S0748–7983:00430–4.
Sethi N, Sharma A, Mathur A, Singh C, Talwar A. Mixed epithelial and mesenchymal neoplasms of female genital tract: case series in a tertiary cancer centre. Indian J Gynecol Oncolog. 2022;20:71.
Daimon A, Fujiwara S, Tanaka Y, Tanaka T, Ohmichi M. A rare case of ovarian carcinosarcoma with squamous cell carcinoma. J Ovarian Res. 2019;12:32.
Romeo C, Le Saux O, Jacobs M, Joly F, Ferron G, Favier L, et al. Therapeutic challenges in patients with gynecologic carcinosarcomas: analysis of a multicenter national cohort study from the French prospective TMRG network. Cancers (Basel). 2022;14:354.
Kozłowski M, Nowak K, Kordek A, Cymbaluk-Płoska A. Therapeutic management of rare primary ovarian neoplasms: carcinosarcoma, leiomyosarcoma, melanoma and carcinoid. Int J Environ Res Public Health. 2021;18:7819.
Heinzelmann-Schwarz V, Kind AB, Vetter M, Russell K, Omar S, Schoetzau A, et al. Should MMMT still be treated with adjuvant taxane-based combination chemotherapy? J Cancer Res Clin Oncol. 2020;146:695–704.
Tani R, Hori T, Yamamoto H, Harada H, Yamamoto M, Yamada M, et al. Aggressive resection of malignant paraaortic and pelvic tumors accompanied by arterial reconstruction with synthetic arterial graft. Am J Case Rep. 2021;22: e931569.
Zuhdy M, Alghandour R, Abdelazeem G, Saleh GA, Saleh MM, Hamdy M, et al. Axillary nodal metastasis in ovarian cancer: a report of three cases and review of literature. J Egypt Natl Canc Inst. 2019;31:9.
Flint M, Velasquez J, Carr C, Kolev V, Zakashansky K. Metastatic ovarian carcinosarcoma in a patient undergoing in-vitro fertilization: a case report. Int J Surg Case Rep. 2023;104: 107937.
Liu Y, Ni M, Huang F, Gu Q, Xiao Y, Du X. Neoadjuvant chemotherapy in advanced epithelial ovarian cancer by histology: a SEER based survival analysis. Medicine (Baltimore). 2023;102: e32774.
Aoki M, Takaya H, Otani T, Nakai H, Murakami K, Matsumura N. Ovarian teratoid carcinosarcoma with a PIK3CA mutation: a case report and review of the literature. Int Cancer Conf J. 2022;11:231–7.
Fox C, Allen N, Schimp V, Maksem J. Ovarian teratoid carcinosarcoma is an aggressive tumor of probable mullerian derivation with a carcinosarcomatous and mixed germ-cell morphology. Case Rep Oncol. 2019;12:241–7.
NCT05619913. EPOCH: eribulin and pembrolizumab in ovarian/uterine carcinosarcoma. 2022. https://clinicaltrials.gov/show/NCT05619913 Accessed 01 Jul 2023.
NCT03740165. Study of Chemotherapy With Pembrolizumab (MK-3475) Followed by maintenance with olaparib (MK-7339) for the first-line treatment of women with BRCA non-mutated advanced epithelial ovarian cancer (EOC) (MK-7339-001/KEYLYNK-001/ENGOT-ov43/GOG-3036). 2023. https://clinicaltrials.gov/ct2/show/NCT03740165 Accessed 03 Jul 2023.
NCT05265793. Camrelizumab combined with apatinib in the treatment of advanced sarcomatoid carcinoma or carcinosarcoma. 2022. https://clinicaltrials.gov/ct2/show/NCT05265793 Accessed 03 Jul 2023.
NCT04969887. Combination immunotherapy in rare cancers under investigation (MOST-CIRCUIT). 2023. https://clinicaltrials.gov/ct2/show/NCT04969887 Accessed 03 Jul 2023.
NCT05224999. Nivolumab for recurrent/metastatic carcinosarcoma. 2022. https://clinicaltrials.gov/ct2/show/NCT05224999 Accessed 03 Jul 2023.
NCT05044871. Biomarker-driven targeted therapy in patients with recurrent platinum-resistant epithelial ovarian cancer (BRIGHT). 2022. https://clinicaltrials.gov/ct2/show/NCT05044871 Accessed 03 Jul 2023.
NCT03651206. Recurrent ovarian carcinosarcoma anti-pd-1 niraparib (ROCSAN). 2023. https://clinicaltrials.gov/ct2/show/NCT03651206 Accessed 03 Jul 2023.
NCT03737643. Study record durvalumab treatment in combination with chemotherapy and bevacizumab, followed by maintenance durvalumab, bevacizumab and olaparib treatment in advanced ovarian cancer patients (DUO-O). 2021. https://clinicaltrials.gov/study/NCT03737643 Accessed 03 Jul 2023.
NCT04919629. APL-2 and pembrolizumab versus APL-2, pembrolizumab and bevacizumab versus bevacizumab alone for the treatment of recurrent ovarian, fallopian tube, or primary peritoneal cancer and malignant effusion. 2023. https://clinicaltrials.gov/ct2/show/NCT04919629 Accessed 03 Jul 2023.
NCT03648489. Dual mTorc inhibition in advanced/recurrent epithelial ovarian, fallopian tube or primary peritoneal cancer (of clear cell, endometrioid and high grade serous type, and carcinosarcoma) (DICE). 2022. https://clinicaltrials.gov/ct2/show/NCT03648489 Accessed 03 Jul 2023.
Han Y, Liu D, Li L. PD-1/PD-L1 pathway: current researches in cancer. Am J Cancer Res. 2020;10:727–42.
Jiang X, Wang J, Deng X, Xiong F, Ge J, Xiang B, et al. Role of the tumor microenvironment in PD-L1/PD-1-mediated tumor immune escape. Mol Cancer. 2019;18:10.
Alsaab HO, Sau S, Alzhrani R, Tatiparti K, Bhise K, Kashaw SK, et al. PD-1 and PD-L1 Checkpoint signaling inhibition for cancer immunotherapy: mechanism, combinations, and clinical outcome. Front Pharmacol. 2017;8:561.
Jiang Y, Chen M, Nie H, Yuan Y. PD-1 and PD-L1 in cancer immunotherapy: clinical implications and future considerations. Hum Vaccin Immunother. 2019;15:1111–22.
Alwosaibai K, Aalmri S, Mashhour M, Ghandorah S, Alshangiti A, Azam F, et al. PD-L1 is highly expressed in ovarian cancer and associated with cancer stem cells populations expressing CD44 and other stem cell markers. BMC Cancer. 2023;23:13.
Chen S, Zhang Z, Zheng X, Tao H, Zhang S, Ma J, et al. Response efficacy of PD-1 and PD-L1 inhibitors in clinical trials: a systematic review and meta-analysis. Front Oncol. 2021;11: 562315.
Monk BJ, Colombo N, Oza AM, Fujiwara K, Birrer MJ, Randall L, et al. Chemotherapy with or without avelumab followed by avelumab maintenance versus chemotherapy alone in patients with previously untreated epithelial ovarian cancer (JAVELIN Ovarian 100): an open-label, randomised, phase 3 trial. Lancet Oncol. 2021;22:1275–89.
Pujade-Lauraine E, Fujiwara K, Ledermann JA, Oza AM, Kristeleit R, Ray-Coquard IL, et al. Avelumab alone or in combination with chemotherapy versus chemotherapy alone in platinum-resistant or platinum-refractory ovarian cancer (JAVELIN Ovarian 200): an open-label, three-arm, randomised, phase 3 study. Lancet Oncol. 2021;22:1034–46.
Heitz F, Ataseven B, Staniczok C, Denkert C, Rhiem K, Hahnen E, et al. Implementing HRD testing in routine clinical practice on patients with primary high-grade advanced ovarian cancer. Cancers (Basel). 2023;15:818.
Ngoi NYL, Tan DSP. The role of homologous recombination deficiency testing in ovarian cancer and its clinical implications: do we need it? ESMO Open. 2021;6: 100144.
Mamdani H, Matosevic S, Khalid AB, Durm G, Jalal SI. Immunotherapy in lung cancer: current landscape and future directions. Front Immunol. 2022;13: 823618.
Adeleke S, Okoli S, Augustine A, Galante J, Agnihotri A, Uccello M, et al. Melanoma-the therapeutic considerations in the clinical practice. Ann Palliat Med. 2023;apm-22–1432.
Boussios S, Rassy E, Samartzis E, Moschetta M, Sheriff M, Pérez-Fidalgo JA, et al. Melanoma of unknown primary: new perspectives for an old story. Crit Rev Oncol Hematol. 2021;158: 103208.
Rhea LP, Mendez-Marti S, Kim D, Aragon-Ching JB. Role of immunotherapy in bladder cancer. Cancer Treat Res Commun. 2021;26: 100296.
Kathuria-Prakash N, Drolen C, Hannigan CA, Drakaki A. Immunotherapy and metastatic renal cell carcinoma: a review of new treatment approaches. Life (Basel). 2021;12:24.
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Study conception and design: A.I and S.B. Administrative support: A.I, S.C, and S.B. Provision of study materials or patients: S.B. Collection and assembly of data: A.I, S.C, and S.B. Data analysis and interpretation: A.I and S.C. Manuscript writing: A.I, S.C, and S.B. Final approval of manuscript: all authors. All authors have read and agreed to the published version of the manuscript.
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Ismail, A., Choi, S. & Boussios, S. Frontiers of Ovarian Carcinosarcoma. Curr. Treat. Options in Oncol. 24, 1667–1682 (2023). https://doi.org/10.1007/s11864-023-01138-4
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DOI: https://doi.org/10.1007/s11864-023-01138-4