Abstract
Background
Methanol toxicity is one of the major public health problems because it can cause severe morbidity and mortality. Methanol intoxication causes changes in the balance between the production of free radicals and antioxidant capacity.
Aims
We aimed to investigate the effects of caffeic acid phenethyl ester (CAPE) on the total oxidant status, total antioxidant status (TAS), and oxidative stress index (OSI) parameters of the liver and the serum in a rat model of acute methanol intoxication.
Materials and methods
Rats were treated with intraperitoneal (i.p.) Methotrexate (MTX) for 7 days. On the 8th day, i.p. Methanol was administered in the methanol, ethanol and CAPE groups. Four hours after methanol treatment, ethanol was injected i.p. in the ethanol group; CAPE (i.p.) in the CAPE group; serum physiologic i.p. in other groups. After 8 hours, rats were killed and the serum and the liver samples were obtained for biochemical analyses.
Results
The OSI value was significantly higher in the methanol group compared to the ethanol and CAPE groups. Serum TAS levels of the methanol group were significantly different compared to the control group, but not compared to the MTX group. The amelioration of oxidative stress was greater in the CAPE group compared to the ethanol group but was not statistically significant.
Conclusion
This study demonstrates that CAPE treatment ameliorates oxidative stress in the serum and liver in a rat model of acute methanol intoxication.
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References
Tephly TR (1991) The toxicity of methanol. Life Sci 48:1031–1041
Eells JT, Salzman MM, Lewandowski MF, Murray TG (1996) Formate-induced alterations in retinal function in methanol-intoxicated rats. Toxicol Appl Pharmacol 140:58–69
Poisoning AAoCTAHCotTGfM, Barceloux DG, Randall Bond G, Krenzelok EP, Cooper H, Allister Vale J (2002) American Academy of Clinical Toxicology practice guidelines on the treatment of methanol poisoning. Clin Toxicol 40:415–446
Dudka J, Burdan F, Szumilo J, Tokarska E, Korobowicz A, Klepacz R, Gieroba R, Madej B, Korobowicz E (2005) Effect of selected alcohol dehydrogenase inhibitors on human hepatic lactate dehydrogenase activity—an in vitro study. J Appl Toxicol 25:549–553
Blagova O, Budantsev A, Sytinsky I, Lajtha A (1982) Changes of neurochemical and electrophysiological indices of rat brain under ethanol intoxication. Neurochem Res 7:1335–1345
Uzar E, Varol S, Acar A, Firat U, Basarslan S, Evliyaoglu O, Yucel Y, Alp H, Gökalp O (2014) Assesment the role of oxidative stres and efficacy of caffeic acid phenethyl ester (CAPE) on neurotoxicity induced by isoniazid and ethambutol in a rat model. Eur Rev Med Pharmacol Sci 18:2953–2959
Sahin A, Kürsat Cingü A, Kaya S, Türkcü G, Ari S, Evliyaoglu O, Çinar Y, Türkcü FM, Yüksel H, Murat M (2013) The protective effects of caffeic acid phenethyl ester in isoniazid and ethambutol-induced ocular toxicity of rats. Cutan Ocular Toxicol 32:228–233
Uzar E, Acar A, Firat U (2011) Protective effect of caffeic acid phenethyl ester in Rat Cerebral Ischemia/Reperfusion Damage}. Turk Norol Derg 17:131–136
Oğuzhanoğlu E, Andac AC, TÜFEK A, YAVUZ L, URAL H, GÖKALP O (2014) Protective role of caffeic acid phenethyl ester on serum cholinesterase inhibition by acute exposure to diazinon in rats. Turk J Med Sci 44:115–120
Şahin A, Kaya S, Türkcü G, Cingü AK, Yüksel H, Türkcü FM, Yüksel H, Çınar Y, Arı Ş, Şahin M (2013) The effects of caffeic acid phenethyl ester in acute methanol toxicity on rat retina and optic nerve. Cutan Ocular Toxicol 32:263–267
Arıkanoğlu A, Yüksel H, Göçmez C, Uzar E, Acar A, Aluçlu MU (2013) Metonol İntoksikasyonuna Bağlı Serebeller Doku Hasarı Üzerine Kafeik Asit Fenetil Esterin Etkisi: Deneysel Çalışma. Turk J Neurol 19(3):93–96
Gonzalez-Quevedo A, Obregón F, Urbina M, Rousso T, Lima L (2002) Effect of chronic methanol administration on amino acids and monoamines in retina, optic nerve, and brain of the rat. Toxicol Appl Pharmacol 185:77–84
Erel O (2004) A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem 37:277–285
Erel O (2005) A new automated colorimetric method for measuring total oxidant status. Clin Biochem 38:1103–1111
Lim CS, Lank PM (2013) Risk assessment of methanol poisoning in outbreaks not applicable to isolated cases. Clin Toxicol 51:119
Hassanian-Moghaddam H, Nikfarjam A, Mirafzal A, Saberinia A, Nasehi AA, Asl HM, Memaryan N (2014) Methanol mass poisoning in Iran: role of case finding in outbreak management. J Public Health (Oxf) 37(2):354–359
Sa’ed HZ, Al-Jabi SW, Sweileh WM, Awang R, Waring WS (2015) Bibliometric profile of the global scientific research on methanol poisoning (1902–2012). J Occup Med Toxicol 10:17
Chen J, Wang L-X, Xia W (2010) Advancement of methanol poisoning mechanism research. Fa Yi Xue Za Zhi 26:294–296
Watkins W, Goodman J, Tephly T (1970) Inhibition of methanol and ethanol oxidation by pyrazole in the rat and monkey in vivo. Mol Pharmacol 6:567–572
Martin LJ (2011) An approach to experimental synaptic pathology using green fluorescent protein-transgenic mice and gene knockout mice to show mitochondrial permeability transition pore-driven excitotoxicity in interneurons and motoneurons. Toxicol Pathol 39:220–233
Dudka J (2006) The total antioxidant status in the brain after ethanol or 4-methylpyrazole administration to rats intoxicated with methanol. Exp Toxicol Pathol 57:445–448
Kraut JA, Kurtz I (2008) Toxic alcohol ingestions: clinical features, diagnosis, and management. Clin J Am Soc Nephrol 3:208–225
File SE, Moser J, Bagchi D, AKUBUE P, Stohs P (1993) Excretion of malondialdehyde, formaldehyde, acetaldehyde and acetone in the urine of rats following acute and chronic administration of ethanol. Alcohol Alcohol 28:287–295
El-Bakary AA, El-Dakrory SA, Attalla SM, Hasanein NA, Malek HA (2009) Ranitidine as an alcohol dehydrogenase inhibitor in acute methanol toxicity in rats. Hum Exp Toxicol 29(2):93–101
Kurcer Z, Oğuz E, Iraz M, Fadillioglu E, Baba F, Koksal M, Olmez E (2007) Melatonin improves methanol intoxication-induced oxidative liver injury in rats. J Pineal Res 43:42–49
Pari L, Karthikesan K (2007) Protective role of caffeic acid against alcohol-induced biochemical changes in rats. Fundam Clin Pharmacol 21:355–361
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Yazgan, Ü.C., Elbey, B., Kuş, S. et al. Effect of caffeic acid phenethyl ester on oxidant and anti-oxidant status of liver and serum in a rat model with acute methanol intoxication. Ir J Med Sci 186, 519–523 (2017). https://doi.org/10.1007/s11845-016-1462-2
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DOI: https://doi.org/10.1007/s11845-016-1462-2