Abstract
Oriental orchids of the genus Cymbidium are famous for their flower fragrance and have great value in the horticultural market. To investigate the regulatory mechanisms of fragrance biosynthesis in Cymbidium faberi, the promoters of the CfAOC and CfJMT genes in the methyl jasmonate biosynthesis pathway were cloned and analyzed. Notably, the CfAOC promoter was transcriptionally active in all vegetative organs, which was not true for the CfJMT promoter, indicating that the latter is tissue-specific for the flowers of C. faberi. The dual-luciferase assay showed that the fragment between –629 and –1038 nt of the CfAOC promoter, as well as the fragment between –960 and –1121 nt of the CfJMT promoter, displayed specific interaction with the CfbHLH transcription factors. These results provide a theoretical basis for the genetic modification of the flower fragrance of Cymbidium based on specific CfbHLH transcription factors.
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (Grant No. 31700626 and 82060684), Science and Technology Research Project, Education Department of Hubei province (No. B2021290), 1050 Young Talents Project of Jiangxi University of Traditional Chinese Medicine (5141900107) and the Scientific Research Foundation for the First-class Discipline of Chinese Medicine Program of Jiangxi University of Traditional Chinese Medicine (JXSYLXK-ZHYAO023). These funding bodies did not play any role in the design, execution, analysis, and interpretations of data or in writing the manuscript.
Funding
National Natural Science Foundation of China, 31700626, Yin Zhou,82060684, Yanqin Xu, Science and Technology Research Project, Education Department of Hubei province, B2021290, Yin Zhou, 1050 Young Talents Project of Jiangxi University of Traditional Chinese Medicine, 5141900107, Yanqin Xu, Scientific Research Foundation for the First-class Discipline of Chinese Medicine Program of Jiangxi University of Traditional Chinese Medicine, JXSYLXK-ZHYAO023, Yanqin Xu
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Conceived and designed the experiments: YZ and YX. Performed the experiments: ZX, XC, JZ and SW. Analyzed the data: YZ, YX, and ZX. Wrote the paper: YZ. All the authors have read the manuscript and approved for publication.
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Zhou, Y., Xu, Z., Chen, X. et al. Functional characterization of the CfAOC and CfJMT gene promoters related to MeJA biosynthesis in Cymbidium faberi. Plant Biotechnol Rep 17, 243–253 (2023). https://doi.org/10.1007/s11816-022-00780-y
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DOI: https://doi.org/10.1007/s11816-022-00780-y