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Comparative proteomic analysis of host responses to Plasmodiophora brassicae infection in susceptible and resistant Brassica oleracea

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Abstract

Clubroot disease, caused by Plasmodiophora brassicae, is one of the most devastating diseases affecting members of the Brassicaceae family. It is difficult to control by chemical or cultural means, and the molecular mechanisms underlying interactions with Brassica oleracea (cabbage) remain poorly understood. Herein, we used a proteomic approach to investigate B. oleracea–P. brassicae interactions during the early phases of infection in above-ground tissues. Proteins were isolated from the aerial parts of clubroot-susceptible (CT-18) and -resistant (YCR) cabbage cultivars at 5 days after inoculation with P. brassicae or buffer (mock) and resolved by sodium dodecyl sulphate–polyacrylamide gel electrophoresis and two-dimensional gel electrophoresis. A total of 24 differentially modulated proteins were identified in at least two biological replicates, and exhibited altered expression between mock and P. brassicae treatments and/or in the different cabbage cultivars. Most of the identified proteins are involved in oxidative stress, abscisic acid (ABA) metabolism, glucose-mediated signalling and responses to stimuli. Resistant YCR plants harboured an increased abundance of ABA-responsive protein, fructose-bisphosphate aldolase and glucose sensor interaction protein compared with CT-18 plants in both mock and P. brassicae-treated samples, suggesting that they may mediate basal defences against P. brassicae infection in YCR. Specifically, we observed that susceptible (CT-18) plants expressed higher levels of cobalamin-independent methionine synthase than YCR, which may enhance susceptibility of the host. Further investigation of the identified proteins will likely facilitate the identification of key molecular determinants, potentially improving clubroot disease resistance in future cabbage crop species.

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Acknowledgements

This work was supported by the KRIBB Initiative Program funded by the Ministry of Science and ICT (Grant no. NRF-2017R1A2B4012820).

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Authors and Affiliations

  1. Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141, Republic of Korea

    Ju Yeon Moon, Suk-Yoon Kwon, Hye Sun Cho, Hyun-Soon Kim, Jae Sun Moon & Jeong Mee Park

  2. Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, Korea University of Science and Technology (UST), Daejeon, 34141, Republic of Korea

    Ju Yeon Moon, Suk-Yoon Kwon, Hye Sun Cho, Hyun-Soon Kim, Jae Sun Moon & Jeong Mee Park

  3. Department of Plant Bioscience, Pusan National University, Miryang, 627-706, Republic of Korea

    Sun Tae Kim

  4. Center for Eco-Friendly New Materials, Korea Research Institute of Chemical Technology (KRICT), Daejeon, 34114, Republic of Korea

    Gyung Ja Choi

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  1. Ju Yeon Moon
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Moon, J.Y., Kim, S.T., Choi, G.J. et al. Comparative proteomic analysis of host responses to Plasmodiophora brassicae infection in susceptible and resistant Brassica oleracea. Plant Biotechnol Rep 14, 263–274 (2020). https://doi.org/10.1007/s11816-020-00596-8

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