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Sodium nitroprusside mediates seedling development and attenuation of oxidative stresses in Chinese cabbage

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Abstract

Nitric oxide (NO) has been shown to be involved in diverse physiological processes in microbes, animals and plants. In this study, the involvement of NO in the development and possible roles in oxidative stress protection of Chinese cabbage (Brassica rapa subsp. pekinensis cv. Samrack-ulgari) seedlings were investigated. Exogenous application of sodium nitroprusside (SNP) retarded root elongation, while increasing lateral root formation of Chinese cabbage. Plants showed no signs of external stress due to SNP application in true leaves. Cotyledons of 3-week-old Chinese cabbage plants were found to be highly sensitive to SNP application. Treated cotyledons displayed rapid tissue collapse and associated cell death. Although SNP application reduced root growth under normal growth conditions, it also enhanced methyl viologen (MV)-mediated oxidative stress tolerance. Analysis of SNP application to Chinese cabbage leaf disks, revealed SNP-induced tolerance against oxidative stresses by MV and H2O2, and evidence includes prevention of chlorophyll loss, superoxide anion (O2 −) accumulation and lipid peroxidation. This report supports a role for nitric oxide in modulating early seedling development, programmed cell death and stress tolerance in Chinese cabbage.

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Abbreviations

DAB:

3,3′-Diaminobenzidine

MV:

Methyl viologen

NBT:

Nitro blue tetrazolium

NO:

Nitric oxide

PCD:

Programmed cell death

RNS:

Reactive nitrogen species

ROS:

Reactive oxygen species

SNP:

Sodium nitroprusside

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Acknowledgments

We are grateful to Dr. Yiqin Wang (Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, China) and Dr. John P. Moore (Institute for Wine Biotechnology, Stellenbosch University, South Africa) for the helpful discussions and the critical reviewing our manuscript. This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (Grant no. 2009-0074471 to Jeum Kyu Hong).

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  1. Laboratory of Plant Pathology and Protection, Department of Horticultural Sciences, College of Life Sciences and Natural Resources, Jinju National University, 150 Chilam-dong, Jinju, Kyungnam, 660-758, Republic of Korea

    Chang Hyun Sung & Jeum Kyu Hong

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  1. Chang Hyun Sung
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  2. Jeum Kyu Hong
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Correspondence to Jeum Kyu Hong.

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Sung, C.H., Hong, J.K. Sodium nitroprusside mediates seedling development and attenuation of oxidative stresses in Chinese cabbage. Plant Biotechnol Rep 4, 243–251 (2010). https://doi.org/10.1007/s11816-010-0138-z

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