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In vitro plantlet regeneration of “dwarf” Indian olive (Elaeocarpus robustus Roxb.): a fruit plant of Bangladesh

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Abstract

A plantlet regeneration protocol was developed on pot-grown mature plants of Elaeocarpus robustus Roxb. cv. Dwarf from nodal and leaf explants. The best yield of adventitious shoots was achieved from the leaf-derived calli in a modified MS (MMS1, half strength of major salts, full strength of minor salts, and vitamins) medium containing 4.0 μM BA + 4.0 μM Kn + 0.5 μM NAA + 15% coconut water (CW). The shoot multiplication rate was amplified about twofold per culture after the addition of 15% CW to the medium. The rate of shoot multiplication reached maximum at the 5th subculture, and it maintained this rate throughout the 3 subsequent subcultures. The best rooting in vitro was investigated by subculturing the microcuttings in an MMS2 (half strength of both major salts and minor salts and full strength of vitamins) medium containing 1.0 μM IBA in the dark for one initial week at 30°C, followed by subculturing them in a plant-growth regulator (PGR)-free medium in the light. The plantlets raised in vitro were successfully established under ex vitro conditions.

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Abbreviations

BA:

6-Benzyladenine

Kn:

6-Furfurylaminopurine

2,4-D:

2,4-Dichlorophenoxyacetic acid

NAA:

α-Naphthaleneacetic acid

IBA:

Indole-3-butyric acid

CW:

Coconut water

PGR:

Plant-growth regulator

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Authors and Affiliations

  1. United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509, Japan

    Md. Mahabubur Rahman & Rubaiyat Sharmin Sultana

  2. Department of Botany, University of Rajshahi, Rajshahi, 6205, Bangladesh

    Muhammad Nurul Amin

  3. Faculty of Agriculture, Utsunomiya University, Utsunomiya, 321-8505, Japan

    Md. Mahabubur Rahman, Futoshi Ishiguri, Shinso Yokota, Rubaiyat Sharmin Sultana, Yuya Takashima, Kazuya Iizuka & Nobuo Yoshizawa

Authors
  1. Md. Mahabubur Rahman
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  2. Muhammad Nurul Amin
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  3. Futoshi Ishiguri
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  4. Shinso Yokota
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  5. Rubaiyat Sharmin Sultana
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  6. Yuya Takashima
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  7. Kazuya Iizuka
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  8. Nobuo Yoshizawa
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Corresponding author

Correspondence to Nobuo Yoshizawa.

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Rahman, M.M., Amin, M.N., Ishiguri, F. et al. In vitro plantlet regeneration of “dwarf” Indian olive (Elaeocarpus robustus Roxb.): a fruit plant of Bangladesh. Plant Biotechnol Rep 3, 259–266 (2009). https://doi.org/10.1007/s11816-009-0098-3

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  • DOI: https://doi.org/10.1007/s11816-009-0098-3

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