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Rapid discrimination of commercial strawberry cultivars using Fourier transform infrared spectroscopy data combined by multivariate analysis

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Abstract

To determine whether pattern recognition based on metabolite fingerprinting for whole cell extracts can be used to discriminate cultivars metabolically, leaves and fruits of five commercial strawberry cultivars were subjected to Fourier transform infrared (FT-IR) spectroscopy. FT-IR spectral data from leaves were analyzed by principal component analysis (PCA) and Fisher’s linear discriminant function analysis. The dendrogram based on hierarchical clustering analysis of these spectral data separated the five commercial cultivars into two major groups with originality. The first group consisted of Korean cultivars including ‘Maehyang’, ‘Seolhyang’, and ‘Gumhyang’, whereas in the second group, ‘Ryukbo’ clustered with ‘Janghee’, both Japanese cultivars. The results from analysis of fruits were the same as of leaves. We therefore conclude that the hierarchical dendrogram based on PCA of FT-IR data from leaves represents the most probable chemotaxonomical relationship between cultivars, enabling discrimination of cultivars in a rapid and simple manner.

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References

  • Chen L, Carpita NC, Reiter WD, Wilson RH, Jeffries C, McCann MC (1998) A rapid method to screen for cell-wall mutants using discriminant analysis of Fourier transformation infrared spectra. Plant J 16:385–392

    Article  PubMed  CAS  Google Scholar 

  • Congiu L, Chicca M, Cella R, Rossi R, Bernacchia G (2000) The use of random amplified polymorphic DNA (RAPD) markers to identify strawberry varieties: a forensic application. Mol Ecol 9:229–232

    Article  PubMed  CAS  Google Scholar 

  • Degani C, Rowland LJ, Levi A, Hortynski JA, Galletta GJ (1998) DNA fingerprinting of strawberry (Fragaria × ananassa) cultivars using randomly amplified polymorphic DNA (RAPD) markers. Euphytica 102:247–253

    Article  CAS  Google Scholar 

  • Degani C, Rowland LJ, Saunders JA, Hokanson SC, Ogden EL, Golan-Goldhirsh A, Galletta GJ (2001) A comparison of genetic relationship measures in strawberry (Fragaria × ananassa Duch.) based on AFLPs, RAPDs, and pedigree data. Euphytica 117:1–12

    Article  CAS  Google Scholar 

  • Goodacre R, Timmins M, Burton R, Kaderbhai N, Woodward AM, Kell DB, Rooney PJ (1998) Rapid identification of urinary tract infection bacteria using hyperspectral whole-organism fingerprinting and artificial neural networks. Microbiology 144:1157–1170

    Article  PubMed  CAS  Google Scholar 

  • Hernanz D, Recamels A, Melendez-Martinez AJ, Gonzalez-Miret ML, Heredia FJ (2007) Assessment of the differences in the phenolic composition of five strawberry cultivars (Fragaria × ananassa Duch.) grown in two different soilless systems. J Agric Food Chem 55:1846–1852

    Article  PubMed  CAS  Google Scholar 

  • Kim SW, Ban SH, Chung H, Cho SH, Chung HJ, Choi PS, Yoo OJ, Liu JR (2004) Taxonomic discrimination of higher plants by multivariate analysis of Fourier transform infrared spectroscopy data. Plant Cell Rep 23:246–250

    Article  PubMed  CAS  Google Scholar 

  • Kim SW, Ban SH, Jeong SC, Chung HJ, Ko S, Yoo OJ, Liu JR (2007a) Genetic discrimination between Catharanthus roseus cultivars by metabolite fingerprinting using 1H NMR spectra of aromatic compounds. Biotechnol Bioprocess Eng 12:646–652

    Article  CAS  Google Scholar 

  • Kim SW, Cho SH, Chung H, Liu JR (2007b) Genetic discrimination between Catharanthus roseus cultivars by multivariate analysis of Fourier transform infrared spectroscopy data. J Plant Biotechnol 34:201–205

    Article  Google Scholar 

  • Kim SW, Ban SH, Yoo OJ, Liu JR (2007c) Discrimination of higher plant calluses based on embryogenic capacity and taxonomic classification by pyrolysis mass spectrometry. Plant Biotechnol Rep 1:61–65

    Article  Google Scholar 

  • Krishnan P, Kruger NJ, Ratcliffe RG (2005) Metabolite fingerprinting and profiling in plants using NMR. J Exp Bot 56:255–265

    Article  PubMed  CAS  Google Scholar 

  • Kunihisa M, Fukino N, Matsumoto S (2003) Development of cleavage amplified polymorphic sequence (CAPS) markers for identification of strawberry cultivars. Euphytica 134:209–215

    Article  CAS  Google Scholar 

  • Kunihisa M, Fukino N, Matsumoto S (2005) CAPS markers improved by cluster-specific amplification for identification of octoploid strawberry (Fragaria × ananassa Duch.) cultivars, and their disomic inheritance. Theor Appl Genet 110:1410–1418

    Article  PubMed  CAS  Google Scholar 

  • Lopez M (2002) Authentication and classification of strawberry varieties by near infrared spectral analysis of their leaves. In: Cho RK, Davies AMC (eds) Near infrared spectroscopy: Proceedings of the 10th international conference, NIR Publications, Chichester, pp 335–338

  • Nielsen JA, Lovell PH (2000) Value of morphological characters for cultivar identification in strawberry (Fragaria × ananassa). NZ J Crop Hortic Sci 28:89–96

    Google Scholar 

  • Seregely Z, Deak T, Bisztray GD (2004) Distinguishing melon genotypes using NIR spectroscopy. Chemom Intell Lab Syst 72:195–203

    Article  CAS  Google Scholar 

  • Stewart D, Yahiaoui N, McDougall GJ, Myton K, Marque C, Boudet AM, Haigh J (1997) Fourier-transform infrared and Raman spectroscopic evidence for the incorporation of cinnamaldehydes into the lignin of transgenic tobacco (Nicotiana tabacum L.) plants with reduced expression of cinnamyl alcohol dehydrogenase. Planta 201:311–318

    Article  CAS  PubMed  Google Scholar 

  • Timmins EM, Howell SA, Alsberg BK, Noble WC, Goodacre R (1998) Rapid differentiation of closely related Candida species and strains by pyrolysis-mass spectrometry and Fourier transform-infrared spectroscopy. J Clin Microbiol 36:367–374

    PubMed  CAS  Google Scholar 

  • Tyrka M, Dziadczyk P, Hortynski JA (2002) Simplified AFLP procedure as a tool for identification of strawberry cultivars and advanced breeding lines. Euphytica 125:273–280

    Article  CAS  Google Scholar 

  • Wenning M, Seiler H, Scherer S (2002) Fourier-transform infrared microspectroscopy, a novel and rapid tool for identification of yeasts. Appl Environ Microbiol 68:4717–4721

    Article  PubMed  CAS  Google Scholar 

  • Woo YA, Kim HJ, Chung H (1999) Classification of cultivation area of ginseng radix with NIR and Raman spectroscopy. Analyst 124:1223–1226

    Article  CAS  Google Scholar 

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Acknowledgments

This work was supported by grants to JRL from the Crop Functional Genomics Center of the 21st Century Frontier Research Program, the Korea Science and Engineering Foundation through the Plant Metabolism Research Center of the Kyung Hee University funded by the Korea Ministry of Science and Technology, Studies on Molecular Genomics of Marine and Extreme Organisms funded by the Korean Ministry of Marine Affairs and Fisheries, the BioGreen 21 Program funded by the Korean Rural Development Agency, and the KRIBB Research Initiative Program.

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Authors and Affiliations

  1. Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 111 Gwahangno, Yuseong-gu, Daejeon, 305-806, South Korea

    Suk Weon Kim

  2. Plant Genome Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 111 Gwahangno, Yuseong-gu, Daejeon, 305-806, South Korea

    Sung Ran Min, Jonghyun Kim & Jang R. Liu

  3. Nonsan Strawberry Experiment Station, Chungcheong Nam-Do Agricultural Research and Extension Services, Nonsan, Chungnam, 320-862, South Korea

    Sang Kyu Park & Tae Il Kim

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  1. Suk Weon Kim
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  2. Sung Ran Min
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  3. Jonghyun Kim
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  4. Sang Kyu Park
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  5. Tae Il Kim
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  6. Jang R. Liu
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Corresponding author

Correspondence to Jang R. Liu.

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The authors Suk Weon Kim and Sung Ran Min contributed equally to this work.

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Kim, S.W., Min, S.R., Kim, J. et al. Rapid discrimination of commercial strawberry cultivars using Fourier transform infrared spectroscopy data combined by multivariate analysis. Plant Biotechnol Rep 3, 87–93 (2009). https://doi.org/10.1007/s11816-008-0078-z

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  • DOI: https://doi.org/10.1007/s11816-008-0078-z

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