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Induced monoterpene and lignin production in mechanically stressed and fungal elicited cultured Cupressus lusitanica cells

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Abstract

Cultured Cupressus lusitanica cells induced by various stresses are thought to produce different complexes of defense chemicals to optimize defense. To compare the induced products of two stimulations, we investigated the emission of monoterpenes, biosynthesis of β-thujaplicin, and accumulation of lignin in mechanically stressed and fungal elicited cultured C. lusitanica cells. Both mechanical stress and fungal elicitor caused emission of qualitatively similar monoterpene blends indicating de novo biosynthesis of these compounds after stimulation, while mechanical stress alone is sufficient to induce fungal elicitor-related monoterpene emission. Sabinene and limonene were the dominant compounds over the time course in both volatile blends. Although the emitted volatile blends were qualitatively similar, the time course and the relative ratios of the constituents of the volatile blends differed with the type of stimulation. While fungal elicited cells produced significant amounts of β-thujaplicin over the 5-day time course, no β-thujaplicin was observed in the mechanically stressed cells. The production of β-thujaplicin was the main dissimilarity of the induced products of these two treatments, suggesting that synthesis of β-thujaplicin is not a general response to all types of stresses, but is a specific response and serves as a strong toxic compound against already invaded fungus. Significantly higher amounts of lignin accumulations were observed in the fungal elicited and mechanically stressed cells on the 5th day after induction. Based on these results, we suggest the composition of induced products was dependent on the method of stimulation.

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Abbreviations

SPME:

Solid phase microextraction

FID:

Flame ionization detector

GC/MS:

Gas chromatograph/mass spectrometer

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Authors and Affiliations

  1. Department of Forestry and Forest Products Sciences, Faculty of Agriculture, Kyushu University, Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan

    Ransika De Alwis, Koki Fujita & Ken’ichi Kuroda

  2. Department of Bio Environment, Faculty of Agriculture, Yamagata University, 1-23 Wakaba-machi, Tsuruoka, Yamagata, 997-8555, Japan

    Tatsuya Ashitani

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  1. Ransika De Alwis
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  2. Koki Fujita
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  3. Tatsuya Ashitani
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  4. Ken’ichi Kuroda
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Correspondence to Koki Fujita.

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De Alwis, R., Fujita, K., Ashitani, T. et al. Induced monoterpene and lignin production in mechanically stressed and fungal elicited cultured Cupressus lusitanica cells. Plant Biotechnol Rep 3, 57–65 (2009). https://doi.org/10.1007/s11816-008-0074-3

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  • DOI: https://doi.org/10.1007/s11816-008-0074-3

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