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Molecular characterization of sulfate-reducing bacteria community in surface sediments from the adjacent area of Changjiang Estuary

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Abstract

Sulfate-reducing bacteria (SRB), which obtain energy from dissimilatory sulfate reduction, play a vital role in the carbon and sulfur cycles. The dissimilatory sulfite reductase (Dsr), catalyzing the last step in the sulfate reduction pathway, has been found in all known SRB that have been tested so far. In this study, the diversity of SRB was investigated in the surface sediments from the adjacent area of Changjiang Estuary by PCR amplification, cloning and sequencing of the dissimilatory sulfite reductase beta subunit gene (dsrB). Based on dsrB clone libraries constructed in this study, diversified SRB were found, represented by 173 unique OTUs. Certain cloned sequences were associated with Desulfobacteraceae, Desulfobulbaceae, and a large fraction (60%) of novel sequences that have deeply branched groups in the dsrB tree, indicating that novel SRB inhabit the surface sediments. In addition, correlations of the SRB assemblages with environmental factors were analyzed by the linear model-based redundancy analysis (RDA). The result revealed that temperature, salinity and the content of TOC were most closely correlated with the SRB communities. More information on SRB community was obtained by applying the utility of UniFrac to published dsrB gene sequences from this study and other 9 different kinds of marine environments. The results demonstrated that there were highly similar SRB genotypes in the marine and estuarine sediments, and that geographic positions and environmental factors influenced the SRB community distribution.

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Authors and Affiliations

  1. College of Environmental Science and Engineering, Ocean University of China, Qingdao, 266100, P. R. China

    Yu Zhang, Yu Zhen & Tiezhu Mi

  2. Key Laboratory of Marine Environment and Ecology, Ministry of Education, Qingdao, 266100, P. R. China

    Yu Zhang, Yu Zhen & Tiezhu Mi

  3. College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, P. R. China

    Hui He

  4. Key Laboratory of Marine Chemical Theory and Technology, Ministry of Education, Qingdao, 266100, P. R. China

    Zhigang Yu

  5. Function Laboratory for Marine Ecology and Environmental Science, Qingdao National Oceanography Laboratory, Qingdao, 266100, P. R. China

    Yu Zhen & Zhigang Yu

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  1. Yu Zhang
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Correspondence to Yu Zhen.

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Zhang, Y., Zhen, Y., Mi, T. et al. Molecular characterization of sulfate-reducing bacteria community in surface sediments from the adjacent area of Changjiang Estuary. J. Ocean Univ. China 15, 107–116 (2016). https://doi.org/10.1007/s11802-016-2781-7

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