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A heterotrophic nitrification-aerobic denitrification bacterium Halomonas venusta TJPU05 suitable for nitrogen removal from high-salinity wastewater

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Abstract

A novel salt-tolerant heterotrophic nitrification and aerobic denitrification (HN-AD) bacterium was isolated and identified as Halomonas venusta TJPU05 (H. venusta TJPU05). The nitrogen removal performance of H. venusta TJPU05 in simulated water (SW) with sole or mixed nitrogen sources and in actual wastewater (AW) with high concentration of salt and nitrogen was investigated. The results showed that 86.12% of NH4+-N, 95.68% of NO3−-N, 100% of NO2−-N and 84.57% of total nitrogen (TN) could be removed from SW with sole nitrogen sources within 24 h at the utmost. H. venusta TJPU05 could maximally remove 84.06% of NH4+-N, 92.33% of NO3−-N, 92.9% of NO2−-N and 77.73% of TN from SW with mixed nitrogen source when the salinity was above 8%. The application of H. venusta TJPU05 in treating AW with high salt and high ammonia nitrogen led to removal efficiencies of 50.96%, 47.28% and 43.19% for NH4+-N, NO3−-N and TN respectively without any optimization. Furthermore, the activities of nitrogen removal-related enzymes of the strain were also investigated. The successful detection of high level activities of ammonia oxygenase (AMO), hydroxylamine oxidase (HAO), nitrate reductase (NAR) and nitrite reductase (NIR) enzymes under high salinity condition further proved the HN-AD and salt-tolerance capacity of H. venusta TJPU05. These results demonstrated that the H. venusta TJPU05 has great potential in treating high-salinity nitrogenous wastewater.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant No. 22075207) and the Training Program of Innovation and Entrepreneurship for Undergraduates (No. 201910058052).

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Authors and Affiliations

  1. State Key Laboratory of Separation Membrane and Membrane Process, School of Environmental Science and Engineering, Tiangong University, Tianjin, 300387, China

    Quanli Man, Peilian Zhang, Weiqi Huang, Qing Zhu & Xiaoling He

  2. School of Chemistry, Tiangong University, Tianjin, 300387, China

    Xiaoling He

  3. Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Science, Nankai University, Tianjin, 300071, China

    Dongsheng Wei

Authors
  1. Quanli Man
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  2. Peilian Zhang
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  3. Weiqi Huang
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  4. Qing Zhu
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  5. Xiaoling He
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  6. Dongsheng Wei
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Corresponding authors

Correspondence to Xiaoling He or Dongsheng Wei.

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Highlights

• H. venusta TJPU05 showed excellent HN-AD ability at high salinity.

• Successful expression of AMO, HAO, NAR and NIR confirmed the HN-AD ability of TJPU05.

• H. venusta TJPU05 could tolerate high salt and high nitrogen environment.

• H. venusta TJPU05 is a promising candidate for the bio-treatment of AW.

Conflict of Interest

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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Man, Q., Zhang, P., Huang, W. et al. A heterotrophic nitrification-aerobic denitrification bacterium Halomonas venusta TJPU05 suitable for nitrogen removal from high-salinity wastewater. Front. Environ. Sci. Eng. 16, 69 (2022). https://doi.org/10.1007/s11783-021-1503-6

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  • DOI: https://doi.org/10.1007/s11783-021-1503-6

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