Abstract
The incorporation of 14C-aspartate during the imbibition of yellow lupin seeds resulted in the production of 14C-alanine and 14CO2. On the basis of tracer and enzymatic assays, conducted in vitro on the extract obtained from lupin seeds, it is postulated that aspartate can be converted to oxaloacetate, then, by phosphoenolopyruvate and pyruvate to alanine. This pathway can be catalyzed by the following enzymes: aspartate aminotransferase, phosphoenolpyruvate carboxykinase, pyruvate kinase and alanine aminotransferase.
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Abbreviations
- GOT:
-
L-aspartate:2-oxoglutarate aminotransferase
- GPT:
-
L-alanine:2-oxoglutarate aminotransferase
- MA:
-
malate
- OAA:
-
oxaloacetate
- PA:
-
pyruvate
- PEP:
-
phosphoenolpyruvate
- PEPC:
-
phosphoenolpyruvate carboxylase
- PEPCK:
-
phosphoenolpyruvate carboxykinase
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Ratajczak, W., Polcyn, W., Lehmann, T. et al. Metabolism of amino acids in germinating yellow lupin seeds. II. Pathway of conversion of aspartate to alanine during the imbibition. Acta Physiol Plant 20, 123–127 (1998). https://doi.org/10.1007/s11738-998-0002-8
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DOI: https://doi.org/10.1007/s11738-998-0002-8