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Genetic mapping and salt tolerance of a novel D1-allelic mutant of rice (Oryza sativa L.)

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Abstract

Plant height and grain shape are important traits that may affect yield in rice, and they therefore have enormous importance in breeding. A dwarf small-grain mutant (S525) was identified among progeny of the Indica rice restorer line ‘Xida 1B’ (wild type) raised from seeds treated with ethyl methanesulfonate. The dwarf and small-grain phenotypes were stably inherited after multi-generation selfing. Field-grown mutant plants showed the phenotypes of dwarfism, broad leaves, and small round grains. Genetic mapping and sequencing confirmed that S525 was a novel d1-allelic mutant. A single-base transition (G to A) in the functional dwarfism gene D1 at the conjunction site of the 11th intron caused excision or duplication of the 11th exon in the mRNA and resulted in translation of a defective Gα protein. The S525 showed enhanced salt tolerance compared with the wild type (WT), and the expression of genes associated with salt tolerance quantitatively increased in response to treatment with 200 mM NaCl. The S525 may be useful for future investigation of Gα functions and in the breeding of new dwarf rice cultivars.

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References

  • Alain IO, Kshitij P, Vincent D, Miguel AC, Juan BB, Sekar R, George SB, Richard AC, Francisco JB, Mikel G (2017) Molecular mechanism of Gαi activation by non-GPCR proteins with a Gα-binding and activating motif. Nat Commun 8:15163

    Article  CAS  Google Scholar 

  • Amarjeet S, Saroj KJ, Jayram B, Girdhar KP (2015) ABA inducible rice protein phosphatase 2C confers ABA insensitivity and abiotic stress tolerance in Arabidopsis. PLoS One 10(4):e0125168

    Article  CAS  Google Scholar 

  • Ashikari M, Wu JZ, Yano M et al (1999) Rice gibberellin-insen-sitive dwarf mutant gene dwarf1 encodes the a-subunit of GTP-binding protein.Proc Natl Acad Sci USA 96(18):10284–10289

    Article  PubMed  CAS  Google Scholar 

  • Batelli G, Verslues PE, Agius F, Qiu Q, Fujii H, Pan S, Schumaker KS, Grillo S, Zhu JK (2007) SOS2 promotes salt tolerance in part by interacting with the vacuolar H+-ATPase and upregulating its transport activity. Mol Cell Biol 27:7781–7790

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Chinnusamy V, Zhu J, Zhu JK (2006) Salt stress signaling and mechanisms of plant salt tolerance. Genet Eng (NY) 27:141–177

    Article  CAS  Google Scholar 

  • Ferrero-Serrano A, Assmann SM (2016) The alpha-subunit of the rice heterotrimeric G protein, RGA1, regulates drought tolerance during the vegetative phase in the dwarf rice mutant d1. J Exp Bot 67(11):3433–3443

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Guo YM, Lu YX, Tan CY et al (2004) Comparative studies on main agronomic traits in Indica-Japonica restore lines under different environment. Southwest China J Agric Sci 17(4):422–425

    Google Scholar 

  • Hong Z, Ueguchi-Tanaka M, Umemura K et al (2003) A rice brassinosteroid-deficient mutant,Ebius dwarf1(d2),is caused bv a loss of function of a new member of cyto-chrome P450. Plant Cell 15:2900–2910

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Hu XM, Qian Q, Xu T, Zhang YE, Dong GJ, Gao TG, Xie Q, Xue YB (2013) The U-Box E3 ubiquitin ligase TUD1 functions with a heterotrimeric Gα subunit to regulate brassinosteroid-mediated growth in rice. PLoS Genet 9(3):e1003391(

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Itoh H, Ueguelli Tanaka M, Sentoku N et al (2001) Cloning and functional analysis of two gibberellin 3p-hydroxylase genes that are differently expressed during the growth of rice.Proc Natl Acad sci USA 98(15):8909–8914

    Article  PubMed  CAS  Google Scholar 

  • Itoh H, Tatsumi T, Sakamoto T, Otomo K, Toyomasu T, Kitano H, Ashikari M, Ichihara S, Matsuoka M (2004) A rice semi-dwarf gene,Tan-Ginbozu (D35), encodes the gibberellin biosynthesis enzyme, ent-kaurene oxidase. Plant Mol Biol 54:533–547

    Article  PubMed  CAS  Google Scholar 

  • Jones HD, Smith SJ, Desikan R, Plakidou-Dymock S, Lovegrove A, Hooley R (1998) Heterotrimeric G proteins are implicated in gibberellin induction of alpha-amylase gene expression in wild oat aleurone. Plant Cell 10(2):245–253

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Katsuyuki O, Noriko I, Hidemi K et al (2009) Study of novel d1 alleles, defective mutant of the α subunit of heterotrimeric G-protein in rice. Genes Genet Syst 84:35–42

    Article  Google Scholar 

  • Komatsu S, Yang G, Hayashi N, Kaku H, Umemura K, Iwasaki Y (2004) Alterations by a defect in a rice G protein a subunit in probenazole and pathogen-induced responses. Plant Cell Environ 27:947–957

    Article  CAS  Google Scholar 

  • Liang GH, Cao XY, Sui JM, Zhao XQ, Yan CJ, Yi CD, Gu MH (2004) Fine mapping of a semidwarf gene sd-g in indica rice (Oryza sativa L.). Chin Sci Bull 49(9):900–904

    CAS  Google Scholar 

  • Oki K, Inaba N, Kitano H, Takahashi S, Fujisawa Y, Kato H, Iwasaki Y (2009a) Study of novel d1 alleles, defective mutants of the alpha subunit of heterotrimeric G-protein in rice. Genes Genetic Systems 84(1):35–42

    Article  PubMed  CAS  Google Scholar 

  • Oki K, Inaba N, Kitagawa K, Fujioka S, Kitano H, Fujisawa Y, Kato H, Iwasaki Y (2009b) Function of the α subunit of rice heterotrimeric G protein in Brassinosteroid signaling. Plant Cell Physiol 50:161–172

    Article  PubMed  Google Scholar 

  • Pandey S, Assmann SM (2004) The Arabidopsis putative G proteincoupled receptor GCR1 interacts with the G protein a subunit GPA1 and regulates abscisic acid signaling. Plant Cell 16:1616–1632

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Sakamoto T, Morinaka Y, Ishiyama K, Kobayashi M, Itoh H, Kayano T, Lwahori S, Matsouka M, Tanaka H (2003) Genetic manipulation of gibberellin metabolism in transgenic rice. Nat Biotechnol 1:909–913

    Article  CAS  Google Scholar 

  • Sasaki A, Ashikari M, Ueguchi-Tanaka M, Itoh H, Nishimura A, Swapan D, Ishiyama K, Saito T, Kobayashi M, Khush GS, Kitano H, Matsuoka M (2002) Green revolution: a mutant gibberellin-synthesis gene in rice. Nature 416:701–702

    Article  PubMed  CAS  Google Scholar 

  • Seo HS, Kim HY, Jeong JY, Lee SY, Cho MJ, Bahk JD (1995) Molecular cloning and characterization of RGA1 encoding a G protein alpha subunit from rice (Oryza sativa L. IR-36). Plant Mol Biol 27:1119–1131

    Article  PubMed  CAS  Google Scholar 

  • Shimada A, Ueguchi-Tanaka M, Sakamoto T (2006) The rice spindly gene functions as a negative regulator of gibberellins signaling by controlling the suppressive function of the DELLA protein, SLR1, and modulating brassinosteroid synthesis. Plant J 48:390–402

    Article  PubMed  CAS  Google Scholar 

  • Ueguchi-Tanaka M, Fujisawa Y, Kobayashi M, Ashikari M, Iwasaki Y, Kitano H, Matsuoka M (2000) Rice dwarf mutant d1, which is defective in the alpha subunit of the heterotrimeric G protein, affects gibberellin signal transduction. Proc Natl Acad Sci USA 97(21):11638–11643

    Article  PubMed  CAS  Google Scholar 

  • Ullah H, Chen JG, Temple B, Boyes DC, Alonso JM, Davis KR, Ecker JR, Jones AM (2003) The b-subunit of the Arabidopsis G protein negatively regulates auxin-induced cell division and affects multiple developmental processes. Plant Cell 15:393–409

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Urano D, Chen JG, Botella JR, Jones AM (2013) Heterotrimeric G protein signalling in the plant kingdom. Open Biol 3(3):120186

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Wang XQ, Ullah H, Jones AM, Assmann SM (2001) G protein regulation of ion channels and abscisic acid signaling in Arabidopsis guard cells. Science 292:2070–2072

    Article  PubMed  CAS  Google Scholar 

  • Wang L, Xu YY, Ma QB, Li D, Xu ZH, Chong K (2006) Heterotrimeric G protein alpha subunit is involved in rice brassinosteroid response. Cell Res 16(12):916–922

    Article  PubMed  CAS  Google Scholar 

  • Wang CH, Ma J, Wang S, Tian P, Qi CY, Zhao ZC, Wang JL, Wang J, Cheng ZJ, Zhang X, Guo XP, Lei CL (2016) Genetic identification of a new D1-allelic mutant and analysis of its gene function in rice. Acta Agron Sin 42(9):1261–1272

    Article  Google Scholar 

  • Yamamuro C.,Ihara Y, Wu X et al (2000) Loss of function of a rice brassinosteroid insensitive homolog prevents inter—node elongation and bending of the lamina joint. Plant Cell 12:1591–1605

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Ye H, Du H, Tang N, Li Xh, Xiong LZ (2009) Identification and expression profiling analysis of TIFY family genes involved in stress and phytohormone responses in rice. Plant Mol Biol 71(3):291–305

    Article  PubMed  CAS  Google Scholar 

  • Zhang JF, Xing SJ, Zhang XD (2004) Principles and practice of forestation in saline soil in China. Chin For Sci Technol 3(2):70–74

  • Zuo JR, Li JY (2014) Molecular genetic dissection of quantitative trait loci regulating rice grain size. Annu Rev Genet 48:99–118

    Article  PubMed  CAS  Google Scholar 

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Acknowledgements

This work was supported by Chongqing Science and Technology Key Project (cstc2017shms-xdny80057) and the Fundamental Research Funds for the Central Universities (XDJK2015C118).

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Authors

Contributions

The contribution of each author to the manuscript: XS and WT planned and designed the research. WT, XZ, XW, and JX performed experiments. XS and WT conducted fieldwork. YL, YS, YT, and YX analyzed data. XS, WT, and XZ wrote the manuscript.

Corresponding author

Correspondence to Xian-chun Sang.

Additional information

Communicated by H. Peng.

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Tian, Wj., Zhang, Xq., Wang, Xw. et al. Genetic mapping and salt tolerance of a novel D1-allelic mutant of rice (Oryza sativa L.). Acta Physiol Plant 40, 153 (2018). https://doi.org/10.1007/s11738-018-2717-5

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  • DOI: https://doi.org/10.1007/s11738-018-2717-5

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