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Antioxidant responses under jasmonic acid elicitation comprise enhanced production of flavonoids and anthocyanins in Jatropha curcas leaves

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Abstract

Jatropha curcas has significant potential for production of biodiesel and secondary metabolites with medical applications. The effect of jasmonic acid (JA) on flavonoid production and antioxidant responses in two Mexican J. curcas plants (accessions I-64 and I-52), growing under controlled environmental conditions, was studied. Foliar application of JA (0.25, 0.5, and 1.0 mM) increased flavonoid content by 3.3-fold (445.6 ± 62.2 µg QE g−1 DW) at 1.0 mM in the I-64 accession after 120 h. At this time, individual flavonoid levels of kaempferol, apigenin, and vitexin were 6.8 (106.8 ± 18.6 µg g−1 DW), 4.4 (15.5 ± 1.5 µg g−1 DW), and 8.1 (6.27 ± 1.3 µg g−1 DW) times higher than in the controls, while for the I-52 specimens, the highest increase of flavonoids occurred at 24 h. In treated I-52 plants, enhancement of 58% (74.7 ± 9.2 µg g−1 DW) in anthocyanins occurred after 120 h, while in I-64 plants, they remained similar to controls. Phenylalanine ammonia lyase showed a peak of activity after 120 h in treated I-64 plants, while the I-52 accession showed peaks at 24 and 120 h. Elicited I-64 plants presented H2O2 levels similar to controls with a 30.6% increase in catalase (CAT) and 3.3-fold in ascorbate peroxidase (APX) activities. In elicited I-52 plants, a 2.4-fold increase in H2O2 concentration was related to a 65.6% decrease of CAT and a 1.8-fold increase of APX activities. Therefore, under JA elicitation, J. curcas plants increased antioxidant responses including flavonoid and anthocyanin production to maintain cell redox balance.

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Acknowledgments

This work was supported by CINVESTAV-IPN and CONACYT-Mexico (Grant 222097). GGLC acknowledges CONACYT-Mexico for a doctoral fellowship (52756). Authors wish to thank Dr. Gabriela Luna-Palencia for her advice in chromatographic analysis and Carmen Fontaine for technical support. We are grateful to Drs. Arturo Pérez Vázquez, Eliseo García-Pérez and Ivan Zavala del Angel for donation of seeds from the Germplasm Bank of Colegio de Postgraduados, Campus Veracruz.

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Authors and Affiliations

  1. Departamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Av. Instituto Politécnico Nacional, 2508, 07360, Ciudad de México, Mexico

    Gonzalo G. Lucho-Constantino, Fabiola Zaragoza-Martínez, Teresa Ponce-Noyola, Fernando Esparza-García & Ana C. Ramos-Valdivia

  2. Departamento de Química, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Av. Instituto Politécnico Nacional, 2508, 07360, Ciudad de México, Mexico

    Carlos M. Cerda-García-Rojas

  3. Departamento de Biotecnología, Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional (CEPROBI-IPN), 62730, Yautepec, Morelos, Mexico

    Gabriela Trejo-Tapia

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  1. Gonzalo G. Lucho-Constantino
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  2. Fabiola Zaragoza-Martínez
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  3. Teresa Ponce-Noyola
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  6. Fernando Esparza-García
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Correspondence to Ana C. Ramos-Valdivia.

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Communicated by H. Peng.

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Lucho-Constantino, G.G., Zaragoza-Martínez, F., Ponce-Noyola, T. et al. Antioxidant responses under jasmonic acid elicitation comprise enhanced production of flavonoids and anthocyanins in Jatropha curcas leaves. Acta Physiol Plant 39, 165 (2017). https://doi.org/10.1007/s11738-017-2461-2

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