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Analysis of chlorophyll a fluorescence and proteomic differences of rice leaves in response to photooxidation

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Abstract

This study investigated the effects of increased sunlight on photooxidation of rice leaf mutant 812HS and its wild-type 812S under field conditions. Light is important for plant growth and development. However, when the absorbed energy exceeds the capacity of utilization of photosynthesis, it leads to the accumulation of singlet oxygen molecules and other reactive oxygen species, which causes oxidative damage. Chlorophyll a fluorescence was applied to examine photosystem II photochemistry. The results demonstrated that intensive light had a negative influence on plant photosynthetic processes. However, the electron transport chain was inhibited and energy dissipation was increased, which can minimize photooxidative damage to the optical system. Meanwhile, proteomic analysis showed that the differential expression of proteins in response to photooxidation participated in multiple pathways, including ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) large subunit, RuBisCO large chain precursor, RuBisCO activase, flavodoxin-like quinone reductase 1, l-ascorbate peroxidase S, oxygen-evolving complex protein 1, and glycolate oxidase. The results indicated that photooxidation induced a response in the rice via the stress-related pathway. The aforementioned proteins, identified by two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS), may be very useful in comprehending how plants respond to photooxidation and can be used as characteristics of stress-induced signals. The results of chlorophyll fluorescence parameter analysis demonstrated the negative influence of intense light on plant photosynthetic processes. This was evidenced by the dissipation of excessive energy and the suppression of the electron transport chain to minimize photooxidative damage to the proteins. Future studies should compare the proteomic difference with parallel gene expression and metabolite profiles.

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Abbreviations

2-DE:

Two-dimensional gel electrophoresis

CHAPS:

3-((3-Cholamidopropyl) dimethylammonio)-1-propane sulfonate

Chl:

Chlorophyll

DTT:

Dithiothreitol

IEF:

Isoelectric focusing

IPG:

Immobilized pH gradients

MS:

Mass spectrometry

MALDI-TOF:

Matrix-assisted laser desorption/ionization time-of-flight

1O2 :

Singlet state oxygen

OJIP:

Chl a fluorescence transient

RC:

Reaction centers

TCA:

Tricarboxylic acid

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Acknowledgements

Studies in the Chen Laboratory are supported by the National Natural Science Foundation of China (Grant Nos. 31271621/C1302 and 31671663), the Priority Academic Program Development of Jiangsu Higher Education Institutions, the Youth Natural Science Foundation of Jiangsu Province (Grant No. BK20140916), the Natural Science Research of Jiangsu Higher Education Institutions of China (Grant No. 14KJB180011), and Jiangsu Collaborative Innovation Center for Modern Crop Production.

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Authors and Affiliations

  1. Jiangsu Key Lab of Biodiversity and Biotechnology, School of Life Sciences, Nanjing Normal University, No. 1 Wenyuan Road, Nanjing, 210023, Jiangsu, People’s Republic of China

    Jing Ma, Chunfang Lv, Minli Xu, Peifei Hao, Yuwen Wang, Weijun Shen, Zhiping Gao & Guoxiang Chen

  2. Institute of Food and Crops, Jiangsu Academy of Agricultural Sciences, 50 Zhongling Street, Nanjing, 210014, People’s Republic of China

    Chuangen Lv

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  1. Jing Ma
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  2. Chunfang Lv
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Correspondence to Guoxiang Chen or Chuangen Lv.

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Communicated by Z Miszalski.

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Ma, J., Lv, C., Xu, M. et al. Analysis of chlorophyll a fluorescence and proteomic differences of rice leaves in response to photooxidation. Acta Physiol Plant 39, 46 (2017). https://doi.org/10.1007/s11738-016-2342-0

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  • DOI: https://doi.org/10.1007/s11738-016-2342-0

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