Abstract
Despite many reports on regeneration of Vitis after cryopreservation, there is no cryopreserved collection of its germplasm. Some Vitis genotypes are reported to be recalcitrant to cryopreservation. Droplet vitrification, considered to be an emerging generic method of cryopreservation, has been applied only to a limited extent in Vitis. In the present study, we first tested the toxicity of plant vitrification solution in both axillary and apical buds in six diverse Vitis accessions. Droplet vitrification was then applied using 50 % predicted survival time of apical and axillary buds in vitrification solution after pre-treatment of donor plantlets with salicylic acid, a substance known to have a protective role in abiotic stress responses. Results showed that axillary buds are more tolerant of vitrification solution than apical buds and required longer treatment time. Pre-treatment of donor plantlets with 0.1 mM salicylic acid resulted in a significantly higher protection to cryopreserved buds, but serial dehydration in sucrose alone had little effect. Pre-treatment with salicylic acid enabled successful cryopreservation of previously recalcitrant rootstock 41B, albeit at a low regeneration rate. For other genotypes, cryopreservation of 6–11 explants will be sufficient to regenerate at least one plant at 95 % probability. This is the first report of successful cryopreservation of a set of diverse Vitis genotypes by droplet vitrification, and we show that pre-treatment of donor plantlets with salicylic acid is critical for the success. This research will contribute to conservation of Vitis germplasm in a cost-effective way avoiding the risks associated with field-based collections.
Similar content being viewed by others
References
Alleweldt G, Dettweiler E (1994) The genetic resources of Vitis: world list of grapevine collections, 2nd edn. Geilweilerhof, Siebeldingen
Barba M, Lernia Gd, Carimi F, Carra A, Abbate L, Chiota G (2008) Rescuing autochthonous grape vines thanks to virus elimination. Informatore Agrario Supplemento 64(10):14–16
Bayati S, Shams-Bakhsh M, Moieni A (2011) Elimination of Grapevine Virus A (GVA) by cryotherapy and electrotherapy. J Agric Sci Technol 13:443–450
Ben-Amar A, Daldoul S, Allel D, Reustle G, Mliki A (2013) Reliable encapsulation-based cryopreservation protocol for safe storage and recovery of grapevine embryogenic cell cultures. Sci Hortic 157:32–38
Benelli C, De Carlo A, Engelmann F (2013) Recent advances in the cryopreservation of shoot-derived germplasm of economically important fruit trees of Actinidia, Diospyros, Malus, Olea, Prunus, Pyrus and Vitis. Biotechnol Adv 31(2):175–185. doi:10.1016/j.biotechadv.2012.09.004
Benson EE (2008) Cryopreservation of phytodiversity: a critical appraisal of theory & practice. Crit Rev Plant Sci 27:141–219
Bernard F, Shaker-Bazarnov H, Kaviani B (2002) Effects of salicylic acid on cold preservation and cryopreservation of encapsulated embryonic axes of Persian lilac (Melia azedarach L.). Euphytica 123(1):85–88. doi:10.1023/A:1014416817303
Cao SF, Hu ZC, Wang HO (2009) Effect of salicylic acid on the activities of anti-oxidant enzymes and phenylalanine ammonia-lyase in cucumber fruit in relation to chilling injury. J Hortic Sci Biotech 84:125–130
Carimi F, Pathirana R, Carra A (2011) Biotechnologies for germplasm management and improvement. In: Szabo PV, Shojania J (eds) Grapevines—varieties, cultivation and management. Nova Science Publishers, New York, pp 199–249
Chen S, Liu Z, Cui J, Ding J, Xia X, Liu D, Yu J (2011) Alleviation of chilling-induced oxidative damage by salicylic acid pretreatment and related gene expression in eggplant seedlings. Plant Growth Regul 65(1):101–108. doi:10.1007/s10725-011-9579-9
Dong C-J, Li L, Shang Q-M, Liu X-Y, Zhang Z-G (2014) Endogenous salicylic acid accumulation is required for chilling tolerance in cucumber (Cucumis sativus L.) seedlings. Planta 240:687–700. doi:10.1007/s00425-014-2115-1
Dussert S, Mauro MC, Deloire A, Hamon A, Engelmann F (1991) Cryopreservation of grape embryogenic suspensions. 1. Influence of pretreatment, freezing and thawing conditions. Cryoletters 12:287–298
Dussert S, Engelmann F, Noirot M (2003) Development of probabilistic tools to assist in the establishment and management of cryopreserved plant germplasm collections. Cryoletters 24(3):149–160
Fan H, Wei J, Li T, Li Z, Guo N, Cai Y, Lin Y (2013) DNA methylation alterations of upland cotton (Gossypium hirsutum) in response to cold stress. Acta Physiol Plant 35(8):2445–2453. doi:10.1007/s11738-013-1278-x
Fennell A, Line MJ (2001) Identifying differential tissue response in grape (Vitis riparia) during induction of endodormancy using nuclear magnetic resonance imaging. J Am Soc Hortic Sci 126(6):681–688
Forsline PL, Towill LE, Waddell JW, Stushnoff C, Lamboy WF, McFerson JR (1998) Recovery and longevity of cryopreserved dormant apple buds. J Am Soc Hortic Sci 123(3):365–370
Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50(1):151–158. doi:10.1016/0014-4827(68)90403-5
Ganino T, Silvanini A, Beghe D, Benelli C, Lambardi M, Fabbri A (2012) Anatomy and osmotic potential of the Vitis rootstock shoot tips recalcitrant to cryopreservation. Biol Plant 56(1):78–82
Goebel-Tourand I, Mauro M, Sossountzov L, Miginiac E, Deloire A (1993) Arrest of somatic embryo development in grapevine: histological characterization and the effect of ABA, BAP and Zeatin in stimulating plantlet development. Plant Cell Tissue Organ Cult 33:91–103
Gonzalez-Arnao MT, Panta A, Roca WM, Escobar RH, Engelmann F (2008) Development of large scale application of cryopreservation techniques for shoot and somatic embryo cultures of tropical crops. Plant Cell Tissue Organ Cult 92:1–13
Gonzalez-Benito ME, Martin C, Vidal JR (2009) Cryopreservation of embryogenic cell suspensions of the Spanish grapevine cultivars ‘Albarino’ and ‘Tempranillo’. Vitis 48:131–136
Häggman H, Uosukainen M (2010) Plant cryopreservation in Finland—towards cryobanking. Cryoletters 31(1):83
Höfer M (2015) Cryopreservation of winter-dormant apple buds: establishment of a duplicate collection of Malus germplasm. Plant Cell Tissue Organ Cult 121(3):647–656. doi:10.1007/s11240-015-0735-1
Johnston JW, Harding K, Benson EE (2007) Antioxidant status and genotypic tolerance of Ribes in vitro cultures to cryopreservation. Plant Sci 172(3):524–534
Kalcsits L, Kendall E, Silim S, Tanino K (2009) Magnetic resonance microimaging indicates water diffusion correlates with dormancy induction in cultured hybrid poplar (Populus spp.) buds. Tree Physiol 29(10):1269–1277. doi:10.1093/treephys/tpp062
Kaniuga Z, Sączyńska V, Miśkiewicz E, Garstka M (1999) Changes in fatty acids of leaf polar lipids during chilling and post-chilling rewarming of Zea mays genotypes differing in response to chilling. Acta Physiol Plant 21(3):231–241. doi:10.1007/s11738-999-0037-5
Keller ERJ (2007) Cryopreservation for maintenance of plant germplasm in Germany. Adv Hortic Sci 21(4):228–231
Keller ERJ, Kaczmarczyk A, Senula A (2008) Cryopreservation for plant genebanks—a matter between high expectations and cautious reservation. Cryoletters 29(1):53–62
Kim H-H, Popova E, Shin D-J, Yi J-Y, Kim CH, Lee J-S, Yoon M-K, Engelmann F (2012) Cryobanking of Korean Allium germplasm collections: results from a 10 year experience. Cryoletters 33(1):45–57
Lambardi M, Benelli C, De Carlo A, Ozudogru EA, Previati A, Ellis D (2011) Cryopreservation of ancient apple cultivars of Veneto: a comparison between PVS2-vitrification and dormant-bud techniques. Acta Hortic 908:191–198
Li Y, Liu C, Li T, Wang C, Xiao Y, Zhang L, Jin D, Zhao Y, Wang Z, Cao J, Hao L (2011) Regulatory role of exogenous salicylic acid in the response of Zoysia japonica plants to freezing temperatures: a comparison with cold-acclimatisation. J Hortic Sci Biotechnol 86:277–283
Lynch PT, Siddika A, Johnston JW, Trigwell SM, Mehra A, Benelli C, Lambardi M, Benson EE (2011) Effects of osmotic pretreatments on oxidative stress, antioxidant profiles and cryopreservation of olive somatic embryos. Plant Sci 181(1):47–56. doi:10.1016/j.plantsci.2011.03.009
Marković Z, Chatelet P, Sylvestre I, Kontic JK, Engelmann F (2013) Cryopreservation of grapevine (Vitis vinifera L.) in vitro shoot tips. Cent Eur. J Biol 8(10):993–1000. doi:10.2478/s11535-013-0223-8
Marković Z, Chatelet P, Preiner D, Sylvestre I, Konti KJ, Engelmann F (2014) Effect of shooting medium and source of material on grapevine (Vitis vinifera L.) shoot tip recovery after cryopreservation. Cryoletters 35:40–47
Mason MG, Ross JJ, Babst BA, Wienclaw BN, Beveridge CA (2014) Sugar demand, not auxin, is the initial regulator of apical dominance. Proc Natl Acad Sci USA 111:6092–6097
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Mutlu S, Karadagoglu O, Atici O, Nalbantoglu B (2013) Protective role of salicylic acid applied before cold stress on antioxidative system and protein patterns in barley apoplast. Biol Plant 57(3):507–513. doi:10.1007/s10535-013-0322-4
NBPGR (2015) Tissue culture and cryopreservation unit. http://www.nbpgr.ernet.in/Divisions_and_Units/Tissue_Culture_Cryo.aspx. Accessed 20 May 2015
Nukari A, Uosukainen M, Rokka V-M, Antonius K, Wang Q, Valkonen JPT (2009) Cryopreservation techniques and their application in vegetatively propagated crop plants in Finland. Agric Food Sci 18(2):117–128
Panis B, Garming H, Piette B, Roux N, Swennen R, Van den Houwe I (2010) Banana conservation activities in the Bioversity International Transit Centre (ITC), Belgium. Cryoletters 31(1):76–94
Panis B, Piette B, André E, Van den Houwe I, Swennen R (2011) Droplet vitrification: the first generic cryopreservation protocol for organized plant tissues? Acta Hortic 908:157–163
Pathirana R, McKenzie MJ (2005) Early detection of grapevine leafroll virus in Vitis vinifera using in vitro micrografting. Plant Cell Tissue Organ Cult 81(1):11–18. doi:10.1007/s11240-004-2498-y
Pires AM, Amado C (2008) Interval estimators for a binomial proportion: comparison of twenty methods. REVSTAT–Stat J 6:165–197
Reed BM (2001) Implementing cryogenic storage of clonally propagated plants. Cryoletters 22:97–104
Repka V (2001) Elicitor-stimulated induction of defense mechanisms and defense gene activation in grapevine cell suspension cultures. Biol Plant 44(4):555–565
Sakai A, Kobayashi S, Oiyama I (1990) Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) by vitrification. Plant Cell Rep 9(1):30–33. doi:10.1007/BF00232130
Sayyari M (2012) Improving chilling resistance of cucumber seedlings by salicylic acid. Am Eurasian J Agric Environ Sci 12(2):204–209
Senaratna T, Touchell D, Bunn E, Dixon K (2000) Acetyl salicylic acid (Aspirin) and salicylic acid induce multiple stress tolerance in bean and tomato plants. Plant Growth Regul 30:157–161
Shatnawi M, Anfoka G, Shibli R, Al-Mazra’awi M, Shahrour W, Arebiat A (2011) Clonal propagation and cryogenic storage of virus-free grapevine (Vitis vinifera L.) via meristem culture. Turk J Agric For 35(2):173–184. doi:10.3906/tar-0912-519
Stevens J, Senaratna T, Sivasithamparam K (2006) Salicylic acid induces salinity tolerance in tomato (Lycopersicon esculentum cv. Roma): associated changes in gas exchange, water relations and membrane stabilisation. Plant Growth Regul 49:77–83
Taşgın E, Atıcı Ö, Nalbantoğlu B, Popova LP (2006) Effects of salicylic acid and cold treatments on protein levels and on the activities of antioxidant enzymes in the apoplast of winter wheat leaves. Phytochemistry 67(7):710–715. doi:10.1016/j.phytochem.2006.01.022
Vasanth K, Vivier MA (2011) Improved cryopreservation procedure for long term storage of synchronised culture of grapevine. Biol Plant 55(2):365–369
Wang Q, Tanne E, Amir A, Gafny R (2000) Cryopreservation of in vitro-grown shoot tips of grapevine by encapsulation-dehydration. Plant Cell Tissue Organ Cult 63(1):41–46
Wang QC, Mawassi M, Li P, Gafny R, Sela I, Tanne E (2003) Elimination of Grapevine virus A (GVA) by cryopreservation of in vitro-grown shoot tips of Vitis vinifera L. Plant Sci 165(2):321–327
Wang QC, Mawassi M, Sahar N, Li P, Violeta CT, Gafny R, Sela I, Tanne E, Perl A (2004) Cryopreservation of grapevine (Vitis spp.) embryogenic cell suspensions by encapsulation-vitrification. Plant Cell Tissue Organ Cult 77:267–275
Wang QC, Panis B, Engelmann F, Lambardi M, Valkonen JPT (2009a) Cryotherapy of shoot tips: a technique for pathogen eradication to produce healthy planting materials and prepare healthy plant genetic resources for cryopreservation. Ann Appl Biol 154(3):351–363
Wang Y, Yang ZM, Zhang QF, Li JL (2009b) Enhanced chilling tolerance in Zoysia matrella by pre-treatment with salicylic acid, calcium chloride, hydrogen peroxide or 6-benzylaminopurine. Biol Plant 53(1):179–182. doi:10.1007/s10535-009-0030-2
Yin Z-F, Bi W-L, Chen L, Zhao B, Volk GM, Wang Q-C (2014) An efficient, widely applicable cryopreservation of Lilium shoot tips by droplet vitrification. Acta Physiol Plant 36(7):1683–1692. doi:10.1007/s11738-014-1543-7
Zamecnik J, Faltus M, Bilavcik A (2007) Cryoprotocols used for cryopreservation of vegetatively propagated plants in the Czech cryobank. Adv Hortic Sci 21(4):247–250
Zhao C, Wu Y, Engelmann F, Zhou M (2001) Cryopreservation of axillary buds of grape (Vitis vinifera) in vitro plantlets. Cryoletters 22(5):321–328
Acknowledgments
This work was funded by NZ Winegrowers (NZW 10-107—“Cryopreserved grapevine: a new way to maintain high-health germplasm and cultivar imports with less rigorous quarantine”). This work was part of COST Action 871, CRYOPLANET (Cryopreservation of Crop Species in Europe) approved and funded by European Science Foundation. Travel associated with this work was funded by the Royal Society of New Zealand and COST Action 871. The authors wish to thank Edwige André, Sriya Pathirana and Andrew Mullan for their technical assistance. Tony Baker and John Meyer supplied grapevine material.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that there are no conflicts of interests.
Additional information
Communicated by M. Capuana.
Rights and permissions
About this article
Cite this article
Pathirana, R., McLachlan, A., Hedderley, D. et al. Pre-treatment with salicylic acid improves plant regeneration after cryopreservation of grapevine (Vitis spp.) by droplet vitrification. Acta Physiol Plant 38, 12 (2016). https://doi.org/10.1007/s11738-015-2026-1
Received:
Revised:
Accepted:
Published:
DOI: https://doi.org/10.1007/s11738-015-2026-1