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Comparative proteomic analysis of leaves between photoperiod-sensitive and photoperiod-insensitive maize inbred seedlings under long day treatments

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Abstract

Day length is an important environmental factor affecting the growth and development of maize (Zea mays), a short day (SD) plant grown in different latitudes. Leaf has been recognized as the light perceiving and signal producing organ. Under long day (LD) conditions, photoperiod-sensitive induction phase in maize begins at the fourth fully expanded leaf stage. However, the changes of maize leaf proteome in response to LD are largely unknown. To reveal maize proteome response to LD, proteins extracted from newly expanded fifth, sixth and seventh leaves from maize inbred line 496-10 (photoperiod sensitive) and Huangzao4 (HZ4, photoperiod insensitive) under LD treatments were compared via gel-based proteomic approach. As a result, eleven differentially expressed proteins were identified between 496-10 and HZ4 by mass spectrometry. This difference in protein accumulation was highly reproducible during the fifth to seventh leaf stages and most obvious at the seventh leaf stage. The identified proteins are mainly involved in circadian clock or iron metabolism, light harvesting and photosynthesis, nucleic acid metabolism and carbon fixation or energy metabolism. This study provides new insight into the influences of LD treatment on SD plants, such as maize, at proteome level.

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Fig. 1

Abbreviations

Cab protein:

Chlorophyll a/b binding protein

LD:

Long day

HZ4:

Huangzao4

RuBisCO:

Ribulose-1,5-bisphosphate carboxylase/oxygenase

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

SD:

Short day

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Acknowledgments

This work was supported by the Ministry of Science and Technology of China (grant no. 2011CB111509).

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Authors and Affiliations

  1. State Key Laboratory of Wheat and Maize Crop Science, College of Life Science and College of Agronomy Henan Agricultural University, Zhengzhou, 450002, China

    Ning Wang, Lixia Ku, Yanhui Chen & Wei Wang

Authors
  1. Ning Wang
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  2. Lixia Ku
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  3. Yanhui Chen
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  4. Wei Wang
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Corresponding author

Correspondence to Wei Wang.

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Communicated by M. Stobiecki.

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Wang, N., Ku, L., Chen, Y. et al. Comparative proteomic analysis of leaves between photoperiod-sensitive and photoperiod-insensitive maize inbred seedlings under long day treatments. Acta Physiol Plant 37, 1705 (2015). https://doi.org/10.1007/s11738-014-1705-7

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  • DOI: https://doi.org/10.1007/s11738-014-1705-7

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