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Transgenic poplar “NL895” expressing CpFATB gene shows enhanced tolerance to drought stress

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Abstract

Acyl-ACP thioesterases are responsible for the export of fatty acids produced by the de novo fatty acid synthesis system from the plastid. A fatty acyl–acyl carrier protein thioesterase (CpFATB) was isolated from a Chimonanthus praecox (linn.) Link. (wintersweet) cDNA library. This gene was subsequently transferred into Populus deltoides CL × P. euramericana CL “NL895” through Agrobacterium tumefaciens-mediated leaf disc transformation. The transgenic plants were confirmed by polymerase chain reaction (PCR) analysis and Southern hybridization. Three positive transgenic lines each with a single T-DNA insertion were obtained. Reverse transcription PCR (RT-PCR) confirmed CpFATB expression in transformed plants after short-term exposure to drought. When exposed to drought stress, transgenic plants showed higher superoxide dismutase (SOD) activity, peroxidase (POD) activity, catalase (CAT) activity, chlorophyll a content, chlorophyll b content and total chlorophyll content compared with untransformed plants. Malondialdehyde (MDA) content, electric conductivity and net photosynthetic rate of the transgenic plants were lower than untransgenic plants. The results of physiological indices showed that the overexpression of CpFATB in plants exhibited higher drought stress tolerance under drought stress condition compared to the untransformed control plants. Our data further confirmed and highlighted the functions of CpFATB in enhancing plants drought tolerance and may provide a plausible approach to breed plants combating drought stress and enlarging living scope.

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Acknowledgments

The authors thank Professor Shihong Zhang in Jilin University, for kindly gifted plants expression vector. This work was supported by National High-tech R&D Program of China (2011AA100201-1), Forestry Department of Zhejiang Province (2009SY04) and National Non-profit Research Institute of CAF (CAFYBB2010003-1).

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Xiangshan Road, Beijing, 100091, China

    Ling Zhang, Mingying Liu, Guirong Qiao, Jing Jiang & Renying Zhuo

  2. College of Life Science and Technology, Xinjiang University, Urumqi, 830046, China

    Ling Zhang & Yancheng Jiang

  3. Key Laboratory of Tree Genomics, The Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang, Hangzhou, 311400, Zhejiang, China

    Ling Zhang, Mingying Liu, Guirong Qiao, Jing Jiang & Renying Zhuo

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  1. Ling Zhang
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  2. Mingying Liu
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  3. Guirong Qiao
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  4. Jing Jiang
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  5. Yancheng Jiang
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  6. Renying Zhuo
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Corresponding authors

Correspondence to Yancheng Jiang or Renying Zhuo.

Additional information

Communicated by Y. Wang.

L. Zhang and M. Liu equally contributed to this work.

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Zhang, L., Liu, M., Qiao, G. et al. Transgenic poplar “NL895” expressing CpFATB gene shows enhanced tolerance to drought stress. Acta Physiol Plant 35, 603–613 (2013). https://doi.org/10.1007/s11738-012-1101-0

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  • DOI: https://doi.org/10.1007/s11738-012-1101-0

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