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Proteomic analysis of Arabidopsis protein S-nitrosylation in response to inoculation with Pseudomonas syringae

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Abstract

Nitric oxide (NO) is a key signaling molecule in plants, being its biological effects mainly mediated through S-nitrosylation of cysteine thiols. Using the biotin switch method combined with mass spectrometry analysis we have identified 127 targets of S-nitrosylation in Arabidopsis cell suspension cultures and leaves challenged with virulent and avirulent isolates of Pseudomonas syringae pv. tomato. The NO targets are proteins associated with carbon, nitrogen, and sulpfur metabolism, photosynthesis, the cytoskeleton, stress-, pathogen- and redox-related and signaling proteins. Some proteins were previously identified in plants and mammals, while others (63%) represent novel targets of S-nitrosylation. Our data suggest that NO might be orchestrating the whole plant physiology, presumably through covalent modification of proteins.

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Abbreviations

APX:

Ascorbate peroxidase

DHAR2:

Dehydroascorbate reductase

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

GSNO:

S-nitrosoglutathione

GST:

Glutathion-S-transferase

HR:

Hypersensitive response

MDH:

Malate dehydrogenase

MMTS:

Methyl methanethiosulfonate

NO:

Nitric oxide

PAMPs:

Pathogen-associated molecular patterns

Prx:

Peroxiredoxin

PSII:

Photosystem II

RNS:

Reactive nitrogen species

ROS:

Reactive oxygen species

Rubisco:

Ribulose-1,5-bisphosphate carboxylase/oxygenase

SA:

Salycilyc acid

SAM:

S-adenosyl methionine

SAMS:

S-adenosyl methionine synthetase

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Acknowledgments

This work was carried out with financial support from the Spanish “Ministerio de Educación y Ciencia”, Project BIO-2006-14790. The MS analysis was conducted in the Proteomics Unit (SCAI) at Córdoba University. SEZ is supported by a grant from the the Spanish “Ministerio de Educación y Ciencia”.

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Authors and Affiliations

  1. Agricultural and Plant Biochemistry and Proteomics Research Group, Department of Biochemistry and Molecular Biology, University of Córdoba, Campus de Rabanales, Ed. Severo Ochoa (C6), 14071, Córdoba, Spain

    Ana M. Maldonado-Alconada, Sira Echevarría-Zomeño, Inmaculada Redondo-López & Jesús V. Jorrín-Novo

  2. Institute of Biochemical Plant Pathology, Helmholtz Zentrum München-German Research Center for Environmental Health, 85764, Neuherberg, Germany

    Christian Lindermayr & Jörg Durner

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  1. Ana M. Maldonado-Alconada
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  2. Sira Echevarría-Zomeño
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  3. Christian Lindermayr
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  4. Inmaculada Redondo-López
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  5. Jörg Durner
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  6. Jesús V. Jorrín-Novo
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Correspondence to Ana M. Maldonado-Alconada.

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Communicated by M. Hajduch.

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Maldonado-Alconada, A.M., Echevarría-Zomeño, S., Lindermayr, C. et al. Proteomic analysis of Arabidopsis protein S-nitrosylation in response to inoculation with Pseudomonas syringae . Acta Physiol Plant 33, 1493–1514 (2011). https://doi.org/10.1007/s11738-010-0688-2

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