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Alkaloids biosynthesis by Pancratium maritimum L. shoots in liquid culture

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Abstract

The process of alkaloid biosynthesis by Pancratium maritimum shoot culture, cultivated under submerged conditions, was investigated. Twenty-two compounds of different structural types of the Amaryllidaceae alkaloids (tyramine, narciclasine, galanthamine, haemanthamine, lycorine, pancracine, tazettine and homolycorine types) were detected in the studied samples from biomass and cultural liquid. Dominant compounds in the shoots were of tyramine, lycorine and haemanthamine types, whereas in the culture media were found mainly lycorine type compounds. Based on the multi-metabolic estimation of the alkaloid metabolism and physiological peculiarities, liquid cultures of P. maritimum shoots could be defined as prospective biological systems for producing bioactive molecules with acetylcholinesterase inhibitory and apoptotic activities.

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Abbreviations

2,4-D:

2,4-Dichlorophenoxyacetic acid

AChE:

Acetylcholinesterase

ADB:

Accumulated dry biomass

BAP:

6-Benzylaminopurine

DTNB:

Dithionitrobenzene

GC/MS:

Gas chromatography/mass spectroscopy

MS:

Murashige and Skoog nutrient medium

NAA:

α-Naphthylacetic acid

TIC:

Total ion current

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Acknowledgments

This research has been supported by National Science Fund of Bulgaria under contract number TK-B-1605/2006. The authors acknowledge Ivaila Dincheva (AgroBioInstitute) for preforming GC–MS runs.

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Authors and Affiliations

  1. Laboratory of Applied Biotechnologies, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 139 “Ruski” Blvd., 4000, Plovdiv, Bulgaria

    Vasil Georgiev, Ivan Ivanov & Atanas Pavlov

  2. AgroBioInstitute, 8 Dragan Tzankov Blvd., 1164, Sofia, Bulgaria

    Strahil Berkov

Authors
  1. Vasil Georgiev
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  2. Ivan Ivanov
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  3. Strahil Berkov
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  4. Atanas Pavlov
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Corresponding author

Correspondence to Atanas Pavlov.

Additional information

Communicated by S. Lewak.

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Georgiev, V., Ivanov, I., Berkov, S. et al. Alkaloids biosynthesis by Pancratium maritimum L. shoots in liquid culture. Acta Physiol Plant 33, 927–933 (2011). https://doi.org/10.1007/s11738-010-0622-7

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  • DOI: https://doi.org/10.1007/s11738-010-0622-7

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