Skip to main content
SpringerLink
Log in

Effect of silicon on chilling-induced changes of solutes, antioxidants, and membrane stability in seashore paspalum turfgrass

  • Original Paper
  • Published:
Acta Physiologiae Plantarum Aims and scope Submit manuscript

Abstract

We present a detailed study to investigate if silicon supplementation enhances chilling resistance of seashore paspalum (Paspalum vaginatum Swartz) turf. An enhanced growth status suggests an improved chilling resistance by Si addition, which is coupled with the observation of more Si cells in leaf epidermal cells, as well as a lower LT50 (the low temperature required to cause 50% electrolyte leakage). Chilling stress induces significant adaptive increases of free proline (P < 0.01), all soluble sugar (P < 0.01) and the activity of peroxidase (POD) (P < 0.05), and leads to the decreases of the activities of superoxide dismutase (SOD) and catalase (CAT) (P < 0.05), results in notably higher measurements of malondialdehyde (MDA) (P < 0.05). Silicon addition promoted significant increase of proline and sucrose (P < 0.01), while maintaining significantly higher activities of SOD, POD, CAT, and notably leveling off of MDA (P < 0.05) under chilling stress. These results indicate that silicon enhances the chilling resistance of turfgrass via maintaining a stable membrane and a beneficial cell status readily coping with the chilling-induced oxidative stress.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Agarie S, Hanaoka N, Ueno O, Miyazaki A, Kubota F, Agata W, Kaufman PB (1998) Effects of silicon on tolerance to water deficit and heat stress in rice plants (Oryza sativa L.) monitored by electrolyte leakage. Plant Prod Sci 1:96–103

    Article  Google Scholar 

  • Allen DJ, Ort DR (2001) Impacts of chilling temperatures on photosynthesis in warm-climate plants. Trends Plant Sci 6:36–42

    Article  CAS  PubMed  Google Scholar 

  • Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205–207

    Article  CAS  Google Scholar 

  • Beyer WF, Fridovich I (1987) Assaying for superoxide dismutase activities: some large consequences of minor changes in conditions. Anal Biochem 161:559–566

    Article  CAS  PubMed  Google Scholar 

  • Datnoff LE, Rutherford BA (2003) Accumulation of silicon by bermudagrass to enhance disease suppression of leaf spot and melting out. USGA Turfgrass Environ Res Online 2:1–6

    Google Scholar 

  • Datnoff LE, Seebold KW, Correa Victoria FJ (2001) The use of silicon for integrated disease management: Reducing fungicide applications and enhancing host plant resistance. In: Datnoff LE, Snyder GH, Korndörfer GH (eds) Silicon in agriculture. Elsevier, The Netherlands, pp 171–184

    Chapter  Google Scholar 

  • Dionne J, Castonguay Y, Nadeau P, Desjardins Y (2001) Amino acid and protein changes during cold acclimation of green-type annual bluegrass (Poa annua L.) ecotypes. Crop Sci 41:1862–1870

    Article  CAS  Google Scholar 

  • Dörffling K, Abromeit M, Bradersen U, Dörffling H, Melz G (1998) Involvement of abscisic acid and proline in cold acclimation of winter wheat. In: Li PH, Chen THH (eds) Plant cold hardinesss. Pergamon Press, New York, pp 283–292

    Google Scholar 

  • Duncan RR (1999) Environmentally compatibility of seashore paspalum (saltwater couch) for golf courses and other recreational uses. II. Management protocols. Int Turfgrass Soc Res J 8:1230–1239

    Google Scholar 

  • Epstein E (1999) Silicon. Annu Rev Plant Physiol Plant Mol Biol 50:641–664

    Article  CAS  PubMed  Google Scholar 

  • Eraslan F, Inal A, Pilbeam DJ, Gunes A (2008) Interactive effects of salicylic acid and silicon on oxidative damage and antioxidant activities in spinach (Spinacia oleracea L. cv. Matador) grown under boron toxicity and salinity. Plant Growth Regul 55:207–219

    Article  CAS  Google Scholar 

  • Gao X, Zou C, Wang L, Zhang F (2004) Silicon improves water use efficiency in maize plants. J Plant Nutri 27:1457–1470

    Article  CAS  Google Scholar 

  • Guo W, Zhu YG, Liu WJ, Liang YC, Geng CN, Wang SG (2007) Is the effect of silicon on rice uptake of arsenate related to internal silicon concentrations, iron plaque and phosphate nutrition? Environ Pollut 148:251–257

    Article  CAS  PubMed  Google Scholar 

  • Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts I kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 25:189–198

    Article  Google Scholar 

  • Jing JH, Ding ZR (eds) (1981) Plant biochemistry analytical method. Science Press, Beijing, pp 197–207 (in Chinese)

  • Kaldy MS, Freyman S (1984) Free amino acids in unhardened and cold hardened winter wheat crowns. J Plant Nutr 7:1103–1111

    Article  CAS  Google Scholar 

  • Koster KL, Lynch DV (1992) Solute accumulation and compartmentation during the cold acclimation of Puma rye. Plant Physiol 98:108–113

    Article  CAS  PubMed  Google Scholar 

  • Levitt J (1980) Responses of plants to environmental stresses. In: Chilling, freezing and high temperature stresses, vol 1. Academic Press, New York, p 580

  • Liang YC, Chen Q, Liu Q, Zhang WH, Ding RX (2003) Exogenous silicon (Si) increases antioxidant enzyme activities and reduces lipid peroxidation in roots of salt-stressed barley (Hordeum vulgare L.). J Plant Physiol 160:1157–1164

    Article  CAS  PubMed  Google Scholar 

  • Liang YC, Sun WC, Romheld V (2005a) Effects of foliar- and root-applied silicon on the enhancement of induced resistance to powdery mildew in Cucumis sativus. Plant Pathol 54:678–685

    Article  CAS  Google Scholar 

  • Liang YC, Zhang WH, Chen Q, Ding RX (2005b) Environmental and experimental botany effects of silicon on H+-ATPase and H+-PPase activities, fatty acid composition and fluidity of tonoplast vesicles from roots of salt-stressed barley (Hordeum vulgare L.). Environ Exp Bot 53:29–37

    Article  CAS  Google Scholar 

  • Liang YC, Sun WC, Zhu YG, Christie P (2007) Mechanisms of silicon-mediated alleviation of abiotic stresses in higher plants: a review. Environ Pollut 147:422–428

    Article  CAS  PubMed  Google Scholar 

  • Lyons JM (1973) Chilling injury in plants. Ann Rev Plant Physiol 24:445–466

    Article  CAS  Google Scholar 

  • Ma JF, Yamaji N (2006) Silicon uptake and accumulation in higher plants. Trends Plant Sci 11:392–397

    Article  CAS  PubMed  Google Scholar 

  • Ma JF, Goto S, Tamai K, Ichii M (2001) Role of root hairs and lateral roots in silicon uptake by rice. Plant Physiol 127:1773–1780

    Article  CAS  PubMed  Google Scholar 

  • McKown R, Kuroki G, Warren G (1996) Cold responses of Arabidopsis mutants impaired in freezing tolerance. J Exp Bot 47:1919–1925

    Article  CAS  Google Scholar 

  • Moing A, Carbonne F, Rashad MH, Gaudillère JP (1992) Carbon fluxes in mature peach leaves. Plant Physiol 100:1878–1884

    Article  CAS  PubMed  Google Scholar 

  • Naidu BP, Paleg LG, Aspinall D, Jennings AC, Jones GP (1991) Amino acid and glycine betaine accumulation in cold-stressed wheat seedlings. Phytochemistry 30:407–409

    Article  CAS  Google Scholar 

  • Neethirajan S, Gordon R, Wang L (2009) Potential of silica bodies (phytoliths) for nanotechnology. Trends Biotech 27:461–467

    Article  CAS  Google Scholar 

  • O’Kane D, Gill V, Boyd P, Burdon R (1996) Chilling, oxidative stress and antioxidant enzyme response in Arabidopsis thaliana callus. Planta 198:371–377

    Article  PubMed  Google Scholar 

  • Prychid CJ, Rudall PJ, Gregory M (2004) Systematics and biology of silica bodies in monocotyledons. Bot Rev 69:377–440

    Article  Google Scholar 

  • Ristic Z, Ashworth EN (1993) Changes in leaf ultrastructure and carbohydrates in Arabidopsis thaliana L. (Heyn) cv. Columbia during rapid cold acclimation. Protoplasma 172:111–123

    Article  Google Scholar 

  • Wang SY, Galletta GJ (1998) Foliar application of potassium silicate induces metabolic changes in strawberry plants. J Plant Nutr 21:157–167

    Article  CAS  Google Scholar 

  • Wang L, Qing N, Li M, Zhang F, Zhuang J, Yang W, Li T, Wang Y (2005) Biosilicified structures for cooling plant leaves: a mechanism of highly efficient midinfrared thermal emission. Appl Phys Lett 87:194105

    Article  CAS  Google Scholar 

  • Wanner LA, Junttila O (1999) Cold-induced freezing tolerance in Arabidopsis. Plant Physiol 120:391–399

    Article  CAS  PubMed  Google Scholar 

  • Wei ZW, Yun DH, Wang KS (1995) Measurement of freezing LT50 by electrical conductivity methods in associated with the logistic equation in oats. Qinhai Xumu Shouyi Zhazhi 115:11–13

    Google Scholar 

  • Xia HP, Ao HX, Liu SZ (2000) Reasons why turfgrass becomes yellow and wither in winter of south China and some preventive strategies-taking turf in Guangzhou as an example. Grassl China 2000(5):64–67 (in English with Chinese abstract)

  • Xin Z, Browse J (1998) Eskimo1 mutants of Arabidopsis are constitutively freezing-tolerant. Proc Natl Acad Sci USA 95:7799–7804

    Article  CAS  PubMed  Google Scholar 

  • Yang B, Chen X, Liu X, Guo H (2006) Observation of silicon cells on the leave surface in different varieties of rices. J Chin Electr Microsc Soc 25(2):146–150

    CAS  Google Scholar 

  • Yeo AR, Flowers SA, Rao G, Welfare K (1999) Silicon reduces sodium uptake in rice (Oryza Sativa L.) in saline condition and this is accounted for by a reduction in the transpirational bypass flow. Plant Cell Environ 22:559–565

    Article  CAS  Google Scholar 

  • Zwieniecki MA, Melcher PJ, Holbrook NM (2001) Hydrogel control of xylem hydraulic resistance in plants. Science 291:1059–1062

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

We wish to acknowledge the financial support by the Funds from Foshan Science and Technology Bureau, China (No. 0102010A, 2006A061). We also greatly appreciated the helps from Associate Prof. Liu XL (Department of Mathematic, Foshan University) for data analysis.

Author information

Authors and Affiliations

  1. Faculty of Life Science, Foshan University, 528000, Foshan, Guangdong, People’s Republic of China

    Yongming He, Hongdong Xiao, Huizhen Wang, Yuejin Chen & Min Yu

Authors
  1. Yongming He
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hongdong Xiao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Huizhen Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yuejin Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Min Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Min Yu.

Additional information

Communicated by S. Abe.

Rights and permissions

Reprints and permissions

About this article

Cite this article

He, Y., Xiao, H., Wang, H. et al. Effect of silicon on chilling-induced changes of solutes, antioxidants, and membrane stability in seashore paspalum turfgrass. Acta Physiol Plant 32, 487–494 (2010). https://doi.org/10.1007/s11738-009-0425-x

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11738-009-0425-x

Keywords

Search

Navigation