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Drought stress effects on Rubisco in wheat: changes in the Rubisco large subunit

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Abstract

The leaf protein pattern from drought-tolerant and drought-sensitive wheat varieties subjected to severe soil drought but with the possibility for recover from stress was studied by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). The spots representing Rubisco large subunit (RLS) were identified using polyclonal antibodies against Rubisco and immunoblotting. Some qualitative and quantitative differences in the 2D-PAGE protein map of wheat varieties were revealed under drought conditions. Three days recovery of wheat plants were not enough for restoring RLS quantity to the level of controls after 7 days drought, especially in the drought-sensitive variety Miziya. There are contradictory data in the literature concerning increased or diminished RLS level in drought stressed plants. A comparison of RLS after SDS-PAGE and 2D-PAGE was made. The revealed protein pattern depended on the presence or absence of protease inhibitors in the extraction buffer, on the procedure of extraction, and on the degree of stress.

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Abbreviations

BSA:

Bovine serum albumin

2D-PAGE:

2-Dimensional polyacrylamide gel electrophoresis

DTT:

1,4-Dithio-dl-threitol

DW:

Dry weight

EDTA:

Ethylenediaminetetraacetic acid

EL:

Electrolyte leakage

FW:

Fresh weight

IEF:

Isoelectric focusing

2-ME:

β-Mercapthoethanol

MM:

Molecular mass

PMSF:

Phenylmethanesulfonyl fluoride

Rubisco:

Ribulose-1,5-bisphosphate carboxylase/oxygenase

RLS:

Rubisco large subunit

RSS:

Rubisco small subunit

SDS-PAGE:

Sodium dodecyl sulfate polyacrylamide gel electrophoresis

SL:

Shoot length

TCA:

Trichloracetic acid

TW:

Turgor weight

WD:

Water deficit

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Acknowledgments

This study was supported by grants from Swiss National Science Foundation, SCOPES (project DILPA) and from Ministry of Education and Science of Republic Bulgaria (project CC 1503). The authors are grateful to Dr I. Stancheva for her advices in growing wheat plants in soil and to B. Juperlieva-Mateeva and A. Kostadinova for their excellent technical assistance.

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Authors and Affiliations

  1. Institute of Plant Physiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bdg. 21, 1113, Sofia, Bulgaria

    Klimentina Demirevska & Lyudmila Simova-Stoilova

  2. Institute of Biology and Immunology of Reproduction, Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria

    Diana Zasheva, Rumen Dimitrov & Maria Stamenova

  3. Institute of Plant Sciences, University of Bern, 3013, Bern, Switzerland

    Urs Feller

Authors
  1. Klimentina Demirevska
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  2. Diana Zasheva
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  3. Rumen Dimitrov
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  4. Lyudmila Simova-Stoilova
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  5. Maria Stamenova
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  6. Urs Feller
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Corresponding author

Correspondence to Klimentina Demirevska.

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Communicated by Z. Gombos.

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Demirevska, K., Zasheva, D., Dimitrov, R. et al. Drought stress effects on Rubisco in wheat: changes in the Rubisco large subunit. Acta Physiol Plant 31, 1129–1138 (2009). https://doi.org/10.1007/s11738-009-0331-2

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  • DOI: https://doi.org/10.1007/s11738-009-0331-2

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