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Effect of salinity on antioxidant responses of chickpea seedlings

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Abstract

The changes in the activity of antioxidant enzymes, like superoxide dismutase, ascorbate peroxidase, catalase and glutathione reductase, and growth parameters such as length, fresh and dry weight, proline and H2O2 contents, chlorophyll fluorescence (Fv/Fm), quantum yield of PSII and the rate of lipid peroxidation in terms of malondialdehyde in leaf and root tissues of a chickpea cultivar (Cicer arietinum L. cv. Gökçe) under salt treatment were investigated. Plants were subjected to 0.1, 0.2 and 0.5 M NaCl treatments for 2 and 4 days. Compared to controls, salinity resulted in the reduction of length and of the fresh and dry weights of shoot and root tissues. Salinity caused significant (P < 0.05) changes in proline and MDA levels in leaf tissue. In general, a dose-dependent decrease was observed in H2O2 content, Fv/Fm and quantum yield of photosynthesis under salt stress. Leaf tissue extracts exhibited three activity bands, of which the higher band was identified as MnSOD and the others as FeSOD and Cu/ZnSOD. A significant enhancement was detected in the activities of Cu/ZnSOD and MnSOD isozymes in both tissues. APX and GR activities exhibited significant increases (P < 0.05) in leaf tissue under all stress treatments, whereas no significant change was observed in root tissue. The activity of CAT was significantly increased under 0.5 M NaCl stress in root tissue, while its activity was decreased in leaf tissue under 0.5 M NaCl stress for 4 days. These results suggest that CAT and SOD activities play an essential protective role against salt stress in chickpea seedlings.

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Abbreviations

APX:

ascorbate peroxidase

CAT:

catalase

DW:

dry weight

FW:

fresh weight

GR:

glutathione reductase

MDA:

malondialdehyde

PAGE:

polyacrylamide gel electrophoresis

PSI:

photosystem-I

PSII:

photosystem-II

ROS:

reactive oxygen species

SOD:

superoxide dismutase

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Acknowledgments

The authors thank Prof. Dr. Meral Yücel and Prof. Dr. Hüseyin Avni Öktem for sharing their laboratory facilities during these experiments and for their critical review and discussion of the manuscript.

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Authors and Affiliations

  1. Faculty of Education, Baskent University, 06530, Ankara, Turkey

    Füsun Eyidogan

  2. Department of Biological Sciences, Middle East Technical University, 06531, Ankara, Turkey

    Mehmet Tufan Öz

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  1. Füsun Eyidogan
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  2. Mehmet Tufan Öz
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Correspondence to Füsun Eyidogan.

Additional information

Communicated by W. Bielawski.

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Eyidogan, F., Öz, M.T. Effect of salinity on antioxidant responses of chickpea seedlings. Acta Physiol Plant 29, 485–493 (2007). https://doi.org/10.1007/s11738-007-0059-9

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  • DOI: https://doi.org/10.1007/s11738-007-0059-9

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