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Study on quality evaluation of garidi-5 by fingerprint combined with quantitative analysis of multi-components by single marker (QAMS)

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Abstract

The basis of quality control of Garidi-5 preparations from different manufacturers is provided by qualitative and quantitative analysis of multiple components of Garidi-5. With the combination of fingerprint and QAMS mode, A caprisil C18 (250 mm × 4.6 mm, 5um) column was used with acetonitrile–tetrahydrofuran (25:15) (A)—0.2 mol/NH4Ac (B)—0.2% HAc (C) as mobile phase. The detection wavelength was 220 nm, 235 nm, 254 nm and 270 nm, respectively. The flow volume was 1.0 ml/min with the column temperature standing at 30 ℃, and the injection volume reaching to 10ul. With the High-Performance Liquid Chromatography-Ultraviolet method (HPLC), we established fingerprints of Garidi-5 preparations produced by different manufactures and identified 28 common peaks. Eleven components as Gallic acid, Ellagic acid, Benzoylaconitine, Benzoylhypaconitine, Benzoylmesaconitine, Aconitine, Hypaconitine, Mesaconitine, Costunolide, Dehydrocostuslactone and β-Asarone were identified. There was no significant difference between the results of QAMS method taking a naturally occurring sesquiterpene lactone Costunolide as internal control and that of standard curve. From the perspective of composition, different quality of the same preparation which produced by different manufacturers is achieved. Accuracy, efficiency and feasibility of the experiment were accomplished with the QAMS method, which demonstrated obvious advantages compared with the standard curve method. It can be applied to the quality evaluation of Garidi-5 and lay the experimental foundation for the quality control of Garidi-5.

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The data used to support the findings of this study are available from the corresponding author upon request.

References

  • Bao L, Na S (2020) The traditional concept and modern research profile of mongolian medicine Terminalia Chebula on the detoxification of Radix Aconiti Kusnezoffii. Pharmacol Clinic Chin Materia Medica 36(5):223–227

    Google Scholar 

  • Baosubudao Q (2011) The research about chemical composition and pharmacological action on the mongolian medicine garidi-5. Acta Academiae Medicinae Neimongol 33(6):499–502

    Google Scholar 

  • Bo Y, Guo Ye, Kang S et al (2020) Simultaneous determination of 4 chemical constituents in garidi-5 pill by QAMS method. Environ Chem 39(12):3585–3587

    CAS  Google Scholar 

  • Chulan Cai, Yong Liao. Simultaneous determination of nine components in Shenqi Erxian tablets by QAMS. Chinese Traditional Patent Medicine.2019m41(3):506–511.

  • Chelimuge. Study on the toxicity of Mongolian Medicine Radix Aconiti kusnezoffii and the mechanism of detoxification by baking based on metabonomics and network pharmacology. Beijing University of Chinese Medicine.2020.

  • Dong Yu (2021) Construction of Mongolian medicine quality standard system based on quality markers. China J Tradit Chin Med Pharm 36(1):56–59

    Google Scholar 

  • Fang Liu Pu, Zhang HZ et al (2017) Study on the pharmacodynamic material basis and pharmacological action of Terminalia chebula against rheumatoid arthritis. China Pharm 28(25):3575–3578

    Google Scholar 

  • Han G, Bai Y (2009) Advance for mongolian medicine garidi 5 flavors pill. J Inner Mongolia Univ Natl 24(5):563–565

    Google Scholar 

  • Jia W, Zhang Y, Guo Na et al (2018) Simultaneous determination of five constituents in Roudoukou-8 Powder by QAMS. Chin Tradit Patent Med 40(6):1307–1312

    Google Scholar 

  • Li J, Li S, Ma X et al (2013) Research Progress on chemical constituents and pharmacological effects of Acorus tatarinowii. Chin Tradit Patent Med 35(8):1741–1745

    CAS  Google Scholar 

  • Li J, Liu Q, Xiao L et al (2015) Composition analysis and antibacterial activity detection of volatile oil from Acorus tatarinowii and Acorus calamus in Hunan Province. Chin Tradit Patent Med 37(12):2778–2782

    CAS  Google Scholar 

  • Li Z, Liu Li, He S et al (2020) Simultaneous determination of five Bufadienolides in Liushen pills by QAMS. Chin J Hospital Pharm 40(2):184–188

    Google Scholar 

  • Luo Z, Qiu F, Wei R et al (2012) Research Progress on Application of relative correction factor in multi index determination of traditional Chinese Medicine. Chin Tradit Herbal Drugs 43(7):1448–1452

    CAS  Google Scholar 

  • Luo W, Duan H, Yang Y et al (2016) Determination of 10 components in Artemisia selengensis by QAMS. J Chin Med Mater 39(4):822–825

    CAS  Google Scholar 

  • Shao C, Bai M (2014) Determination of cconitine and neoaconitine in Mongolian medicine Garidi 5 pills by HPLC. J North Pharm 11(12):6–7

    Google Scholar 

  • Eerdun Wang, Tuya, Bagenna, et al. Qualitative study on Mongolian patent medicine Garidi-5 pills. Journal of Medicine & Pharmacy of Chinese Minorities, 1998, 4:65–66.

  • Wang M, Zhou X, Cheng Z (2000) Quality analysis of Mongolian medicine Garidi-5. J Med Pharm Chin Minorities 6:72

    CAS  Google Scholar 

  • Wang L, Xiao H, Liang X (2003) A new method for improving the reproducibility of retention time of chromatographic fingerprints. Chin J Anal Chem 31(10):1232–1236

    CAS  Google Scholar 

  • Wang J, Wang P, Yi Z et al (2016) Research progress on chemical constituents, pharmacological effects and processing compatibility of Terminalia chebula. Inf Tradit Chin Med 33(3):123–126

    Google Scholar 

  • Wang Y, Fan X, Yang J et al (2020) Research progress on terpenes and pharmacological effects of Saussurea lappa. China J Chin Materia Med 45(24):5917–5928

    Google Scholar 

  • Wei Bai, Xuemei Zhou, Xuhong, et al. Determination of Aconitine in Mongolian medicine Garidi-5. Chinese Journal of Ethnomedicine and Ethnopharmacy. 2000,46:300–301.

  • Xuhong, Wei Bai. Determination of Muscone in Mongolian medicine Garidi-5 by Gas chromatography. Journal of Medicine & Pharmacy of Chinese Minorities. 2001,7(1):40

  • Huifeng Zhang, Xuemei Zhou. Determination of Costunolide in Garidi-5 pills by HPLC.Journal of North Pharmacy. 2011,8(11):1–2.

  • Zhang J, Youcheng Xu, Lin D et al (2020) Determination and quality evaluation of four chemical components in different primordial Araceae by QAMS. J Chin Med Mater 43(1):141–144

    Google Scholar 

  • Zhao Q, Feng W, Zhang Q et al (2014) Feasibility study on Determination of multi components in Zhizi Jinhua pills by QAMS. China J Chin Materia Med 39(10):1826–1833

    CAS  Google Scholar 

  • Zhao Lu, Liao C, Yang X et al (2020) Research progress in Terminalia chebula and its predictive analysis on Q-marker. Chin Tradit Herbal Drugs 51(10):2732–2744

    Google Scholar 

  • Zhu Y, Qi J, Boyang Yu (2019) Research progress and thinking of“effect-spectrum integrated fingerprint technology”in the material basis of traditional chinese medicine. Modern Tradit Chin Med Materia Materia-World Sci Technol 21(8):1702–1708

    Google Scholar 

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Funding

This work was supported by the open project of the Inner Mongolia Key Laboratory of Human Genetic Diseases Research, and the construction project of the Key Laboratory of Chifeng University (CFXYZD202008).

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  1. Basic Medicine College, Chifeng University, Chifeng, 024000, Inner Mongolia, China

    Ling Gao

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  1. Ling Gao
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GL contributed to this research idea and was involved in the analysis and preparation of the manuscript and also polished and checked the manuscript.

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Correspondence to Ling Gao.

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Gao, L. Study on quality evaluation of garidi-5 by fingerprint combined with quantitative analysis of multi-components by single marker (QAMS). Chem. Pap. 76, 545–555 (2022). https://doi.org/10.1007/s11696-021-01883-6

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  • DOI: https://doi.org/10.1007/s11696-021-01883-6

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