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Secondary metabolites, antioxidant and anti-proteinase activities of methanolic extracts from cones of hop (Humulus lupulus L.) cultivars

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Abstract

Contents of polyphenols, flavonoids, bitter acids, α-bitter acids, xanthohumol, desmethylxanthohumol, essential oils, antioxidant activities, and inhibitory activities against trypsin, thrombin, urokinase, and papain were analysed in methanolic extracts prepared from the hope cones. Significant differences between extracts from eight hop cultivars in all measured parameters were found. Two cultivars—Agnus and Vital—could be designated as the very special, all possessing high content of bitter acids, α-acids, essential oils, xanthohumol, and desmethylxanthohumol. The other two cultivars—Bohemie and Sládek—had interesting inhibitory activities against analysed proteinases. Differences between cultivars were also in content of total polyphenols, total flavonoids, as well as in antioxidant activity. All analysed hop cultivars developed originally for brewing contained also secondary metabolites with valuable biological activities related to health conditions associated with antioxidant status and some pathophysiological agents of proteinase character.

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Acknowledgements

This work was supported by the Grant No. VEGA 1/0635/13 “Hop as an important source of phytomedically active substances” supported by the Scientific Grant Agency of the Slovak Academy of Sciences and Ministry of Education, Science, Research and Sport of the Slovak Republic.

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Authors and Affiliations

  1. Department of Biotechnology, Faculty of Natural Sciences, University of SS. Cyril and Methodius, Nám. J. Herdu 2, 917 01, Trnava, Slovak Republic

    Tibor Maliar, Erik Krošlák & Ján Kraic

  2. Department of Chemistry, Faculty of Natural Sciences, University of SS. Cyril and Methodius, 917 01, Trnava, Slovak Republic

    Peter Nemeček & Mária Maliarová

  3. Department of Biology, Faculty of Natural Sciences, University of SS. Cyril and Methodius, 917 01, Trnava, Slovak Republic

    Eva Ürgeová

  4. Hop Research Institute, Co., Ltd., 438 01, Žatec, Czech Republic

    Vladimír Nesvadba & Karel Krofta

  5. Institute the Physiotherapy, Balneology and Therapeutic Rehabilitation, University of SS, Cyril and Methodius, 917 01, Trnava, Slovak Republic

    Katarína Vulganová

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  1. Tibor Maliar
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  2. Peter Nemeček
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Correspondence to Ján Kraic.

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Maliar, T., Nemeček, P., Ürgeová, E. et al. Secondary metabolites, antioxidant and anti-proteinase activities of methanolic extracts from cones of hop (Humulus lupulus L.) cultivars. Chem. Pap. 71, 41–48 (2017). https://doi.org/10.1007/s11696-016-0034-2

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  • DOI: https://doi.org/10.1007/s11696-016-0034-2

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