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The Changes of Serum Metabolites in Diabetic GK Rats after Ileal Transposition Surgery

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Abstract

Background

Ileal transposition (IT) surgery could improve metabolism. Metabolomics has been applied comprehensively in analyzing the global dynamic alterations of metabolites. In the present study, we aimed to investigate serum metabolite alterations in diabetic Goto-Kakizaki (GK) rats after IT surgery.

Methods

Male GK rats were subjected to IT and Sham-IT surgery. Six weeks later, body weight, food intake, fat mass, and serum biochemical parameters were measured. The serum metabolomic fingerprint was analyzed using ultra-performance liquid chromatography–mass spectrometry (LC-MS)-based, non-targeted metabolomic approach. The differential metabolites were identified using principal component analysis and orthogonal partial least squares discriminant analysis. Metabolic pathway analysis was performed using HMDB and KEGG databases.

Results

The body weight, food intake, fat mass, serum levels of glucose and insulin, and homeostasis model assessment of insulin resistance (HOMA-IR) of IT rats were significantly decreased when compared with Sham-IT rats (all P < 0.05). In the metabolomics analysis, ten serum differential metabolites were identified. Compared with Sham-IT rats, serum LysoPC(O-18:0) and PG(20:4/20:0) of IT rats were decreased, while genistein 4′-O-glucuronide, 5,6:8,9-Diepoxyergost-22-ene-3,7beta-diol, PI(16:0/18:2(9Z,12Z)), docosapentaenoic acid, 3-Oxo-4,6-choladienoic acid, 3-Oxocholic acid, and TG were increased. Pathway analysis highlighted the following pathways: ether lipid metabolism, alpha linolenic acid and linolenic acid metabolism, incretin synthesis and secretion, free fatty acid receptors, and biosynthesis of unsaturated fatty acids.

Conclusions

IT surgery could significantly decrease body weight and fat mass and improve glucose metabolism in diabetic GK rats. These beneficial effects might be related to the changes of serum metabolites which involved in lipid metabolism, bile acids, and incretin.

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Funding

The study was supported by grants from the National Natural Science Foundation of China (No. 81400797 for Weijie Chen, No. 30600836, 81471024 for Huijuan Zhu, No. 30540036, 30771026, 81370898 for Fengying Gong), the Beijing Natural Science Foundation (No. 7082079, 7182130 for Fengying Gong), the National Key Program of Clinical Science (WBYZ2011-873 for Fengying Gong and Huijuan Zhu), and the PUMCH Foundation (2013-020 for Fengying Gong).

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Authors and Affiliations

  1. Key Laboratory of Endocrinology of National Health Commission, Department of Endocrinology, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, 100730, China

    Kemin Yan, Huijuan Zhu, Hui Pan, Linjie Wang, Hongbo Yang, Meijuan Liu & Fengying Gong

  2. Department of Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, 100730, China

    Weijie Chen & Guole Lin

  3. Core Facility of Instrument, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences/School of Basic Medicine, Peking Union Medical College, Beijing, 100730, China

    Wei Sun & Xiaoyan Liu

Authors
  1. Kemin Yan
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  2. Weijie Chen
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  5. Wei Sun
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  9. Hongbo Yang
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  10. Meijuan Liu
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  11. Fengying Gong
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Corresponding author

Correspondence to Fengying Gong.

Ethics declarations

All animal experimental procedures were approved by the ethics committee of Peking Union Medical College Hospital.

Conflict of Interest

The authors declare that they have no conflict of interest.

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Yan, K., Chen, W., Zhu, H. et al. The Changes of Serum Metabolites in Diabetic GK Rats after Ileal Transposition Surgery. OBES SURG 29, 882–890 (2019). https://doi.org/10.1007/s11695-018-3582-4

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