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Angiotensin I-converting enzyme, dipeptidyl peptidase-IV, and α-glucosidase inhibitory potential of hazelnut meal protein hydrolysates

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Abstract

The objective of this study was to determine the bioactive potential of hazelnut meal protein hydrolysates. Hazelnut meal protein isolate was hydrolyzed using Alcalase and Trypsin + Chymotrypsin to 23.5% and 13.7% degrees of hydrolysis, respectively. The peptide fractions (< 5 kDa and > 5 kDa) were screened for the in vitro inhibition of angiotensin I-converting enzyme (ACE), dipeptidyl peptidase-IV (DPP-IV), and α-glucosidase activities. Peptide fractions > 5 kDa showed a higher potency to inhibit ACE (IC50 = 0.10–0.13 mg/mL), whereas peptide fractions < 5 kDa were more effective in inhibiting DPP-IV (IC50 = 0.37–0.45 mg/mL) and α-glucosidase (IC50 = 3.62–3.89 mg/mL), with no significant difference in treatment with Alcalase and Trypsin + Chymotrypsin. The results of the study showed that hazelnut meal protein is a potential source of bioactive peptide delivery and that the hydrolysates obtained could be used as an alternative ingredient for the development of new functional foods.

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References

  1. H. Korhonen, A. Pihlanto, Int. Dairy J. 16, 945–960 (2006)

    Article  CAS  Google Scholar 

  2. P. Mudgil, B. Jobe, H. Kamal, M. Alameri, N. Al Ahbabi, S. Maqsood, LWT-Food Sci. Technol. 101, 251–258 (2019)

    Article  CAS  Google Scholar 

  3. C. Liu, L. Fang, W. Min, J. Liu, H. Li, Food Chem. 245, 471–480 (2018)

    Article  CAS  Google Scholar 

  4. A. Lambeir, C. Durinx, S. Scharpé, I. De. Meester, Crit. Rev. Cl. Lab. Sci. 40(3), 209–294 (2003)

    Article  CAS  Google Scholar 

  5. L.W. Koh, L.L. Wong, Y.Y. Loo, S. Kasapis, D. Huang, J. Agric. Food Chem. 58(1), 148–154 (2010)

    Article  CAS  Google Scholar 

  6. A.B. Nongonierma, R.J. Fitzgerald, Food Diges. 6, 19–29 (2015)

    CAS  Google Scholar 

  7. Tarım Ürünleri Piyasaları: Fındık (2020) https://arastirma.tarimorman.gov.tr/tepge. Accessed 8 Aug 2020

  8. T. Esposito, F. Sansone, S. Franceschelli, P. Del Gaudio, P. Picerno, R.P. Aquino, T. Mencherini, Int. J. Mol. Sci. 18, 392–404 (2017)

    Article  Google Scholar 

  9. C. Alasalvar, F. Shahidi, J.S. Amaral, B.P.P. Oliveira, in Tree Nuts: Composition, Phytochemicals, and Health Effects. ed. by C. Alasalvar, F. Shahidi (CRC Press, Boca Raton, 2008), p. 185

    Chapter  Google Scholar 

  10. G.M. Sharma, M. Su, A.U. Joshi, K.H. Roux, S.K. Sathe, J. Agric. Food Chem. 58, 5457–5464 (2010)

    Article  CAS  Google Scholar 

  11. F. Tatar, M.T. Tunç, T. Kahyaoglu, J. Food Sci. Technol. 52(2), 1024–1031 (2013)

    Article  Google Scholar 

  12. L.Y. Aydemir, A.A. Gökbulut, Y. Baran, A. Yemenicioǧlu, Food Hydrocolloid 36, 130–142 (2014)

    Article  CAS  Google Scholar 

  13. İ Gülseren, B. Çakır, A.F. Çağlar, J. Food 44(2), 309–317 (2019)

    Google Scholar 

  14. C. Liu, Y. Yu, F. Liu, L. You, Food Nutr. Sci. 10(11), 1374–1387 (2019)

    CAS  Google Scholar 

  15. A.F. Çağlar, B. Çakır, İ Gülseren, Eur. Food Res. Technol. 247, 1189–1198 (2021)

    Article  Google Scholar 

  16. E.Ç. Eroğlu, K. Oztop, S. Aksay, J. Microbiol. Biotechnol. Food Sci. 10(1), 78–82 (2020)

    Google Scholar 

  17. C. Liu, D. Ren, J. Li, L. Fang, J. Wang, J. Liu, W. Min, J. Funct. Foods 42, 203–215 (2018)

    Article  CAS  Google Scholar 

  18. İ Gülseren, J. Food Meas. Charact. 12(4), 2607–2614 (2018)

    Article  Google Scholar 

  19. I. Arcan, A. Yemenicioglu, Food Chem. 103, 301–312 (2007)

    Article  CAS  Google Scholar 

  20. J. Adler-Nissen, J. Chem. Technol. Biotechnol. 34, 215–222 (1984)

    Article  Google Scholar 

  21. AOAC. Official Methods of Analysis of the Association of Official Analytical Chemists, 17th edn. (AOAC International, Maryland, USA, 2000)

  22. S.M. Shalaby, M. Zakora, J. Otte, J. Dairy Res. 73, 178–186 (2006)

    Article  CAS  Google Scholar 

  23. Y. Emre, H. Sevgili, M. Şanlı, Aquac. Res. 39(3), 324–328 (2008)

    Article  CAS  Google Scholar 

  24. F. Shahidi, X.Q. Han, J. Synowiecki, Food Chem. 53, 285–293 (1995)

    Article  CAS  Google Scholar 

  25. M.R. Segura-Campos, F. Peralta-González, A. Castellanos-Ruelas, L.A. Chel-Guerrero, D.A. Betancur-Ancona, Biomed Res. Int. (2013). https://doi.org/10.1155/2013/541947

  26. T. Cucu, C. Platteau, I. Taverniers, B. Devreese, M. De. Loose, B. De. Meulenaer, Food Control 30, 497–503 (2013)

    Article  CAS  Google Scholar 

  27. C. Nitride, G. Picariello, G. Mamone, P. Ferranti, in Proteomics in Food Science: From Farm to Fork, ed. M. L. Colgrave, (Elsevier Academic Press, 2017), pp. 107–122. https://doi.org/10.1016/B978-0-12-804007-2.00007-2

  28. N. Prieto, C. Burbano, E. Iniesto, J. Rodríguez, B. Cabanillas, J.F. Crespo, M.M. Pedrosa, M. Muzquiz, J.C. del Pozo, R. Linacero, C. Cuadrado, Foods 3(2), 279–289 (2014)

    Article  Google Scholar 

  29. F.T. Saricaoglu, O. Gul, A. Besir, I. Atalar, J. Food Eng. 233, 98–108 (2018)

    Article  CAS  Google Scholar 

  30. Y. Guo, D. Pan, M. Tanokura, Food Chem. 114, 328–333 (2009)

    Article  CAS  Google Scholar 

  31. C. Megias, J. Pedroche, M. del Mar Yust, M. Alaiz, J. Giron-Calle, F. Millan, J. Vioque, LWT-Food Sci. Technol. 42, 228–232 (2009)

    Article  CAS  Google Scholar 

  32. H.G. Akıllıoğlu, S. Karakaya, Eur. Food Res. Technol. 229, 915–921 (2009)

    Article  Google Scholar 

  33. E.C. Eroglu, S. Aksay, Ind. J. Pharm. Educ. 51(3), 417–420 (2017)

    Google Scholar 

  34. B.L. White, T.H. Sanders, J.P. Davis, LWT-Food Sci. Technol. 56(2), 537–542 (2014)

    Article  CAS  Google Scholar 

  35. P. Li, J. Jia, M. Fang, L. Zhang, M. Guo, J. Xie, Y. Xia, L. Zhou, D. Wei, Process Biochem. 49(5), 898–904 (2014)

    Article  CAS  Google Scholar 

  36. R. Liu, J. Cheng, H. Wu, Int. J. Mol. Sci. 20, 2–22 (2019)

    Google Scholar 

  37. A.B. Nongonierma, S. Le. Maux, C. Dubrulle, C. Barre, R.J. FitzGerald, J. Cereal Sci. 65, 112–118 (2015)

    Article  CAS  Google Scholar 

  38. L. Mojica, K. Chen, E.G. de Mejia, J. Food Sci. 80, 188–198 (2015)

    Article  Google Scholar 

  39. E.D. Stefano, T. Oliviero, C.C. Udenigwe, Curr. Opin. Food Sci. 20, 7–12 (2018)

    Article  Google Scholar 

  40. R. Vilcacundo, C. Martínez-Villaluenga, B. Hernández-Ledesma, J Funct. Foods 35, 531–539 (2017)

    Article  CAS  Google Scholar 

  41. X. Gu, T. Gao, Y. Hou, D. Li, L. Fu, LWT-Food Sci. Technol. 134, 110215 (2020)

    Article  CAS  Google Scholar 

  42. M. González-Montoya, B. Hernández-Ledesma, R. Mora-Escobedo, C. Martínez-Villaluenga, Int. J. Mol. Sci. 19, 2–14 (2018)

    Article  Google Scholar 

  43. Y. Ren, K. Liang, Y. Jin, M. Zhang, Y. Chen, H. Wu, F. Lai, J. Funct. Foods 26, 439–450 (2016)

    Article  CAS  Google Scholar 

  44. R. Wang, H. Zhao, X. Pan, C. Orfila, W. Lu, Y. Ma, Food Sci. Nutr. 7, 1848–1856 (2019)

    Article  CAS  Google Scholar 

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Funding

I would like to thank Ege University Scientific Research Council for their financial support (Project number: 15-MUH-065).

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  1. Department of Food Engineering, Faculty of Engineering, Ege University, Izmir, Turkey

    Sebnem Simsek

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  1. Sebnem Simsek
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Simsek, S. Angiotensin I-converting enzyme, dipeptidyl peptidase-IV, and α-glucosidase inhibitory potential of hazelnut meal protein hydrolysates. Food Measure 15, 4490–4496 (2021). https://doi.org/10.1007/s11694-021-00994-8

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  • DOI: https://doi.org/10.1007/s11694-021-00994-8

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