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Chemical compositions, total phenolic contents, antimicrobial and antioxidant activities of the extract and essential oil of Thymbra spicata L. growing wild in Turkey

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Abstract

In the study, chemical composition, total phenolic contents, antioxidant capacity and antimicrobial activities of the extract and essential oil of Thymbra spicata L. growing wild in Turkey were investigated. Chemical compositions of T. spicata L. extract and essential oil were determined using the GC–MS method. The main components of the plant extract were carvacrol (88.75%) and acetic acid (11.25%) while the main components of the plant essential oil were carvacrol (56.03%), trans caryophyllene (10.41%), p-cymene (9.61%) and γ-terpinene (6.87%). The total phenolic contents of extract and essential oil were 1350 and 3440 µg GAE g−1, respectively. Antioxidant activities of the samples were tested using DPPH and ABTS+ radical scavenging activities. The antioxidant capacity of the extract and essential oil (50.51–98.28%) is highly correlated with their phenolic contents. The antimicrobial effects of this plant were tested by disc diffusion and broth dilution methods against seven bacteria. The highest inhibition zone values were observed against Staphylococcus aureus as 15 ± 7 mm and 50 ± 10 mm, for extract and essential oil of T. spicata L., respectively. According to broth dilution method, Bacillus subtilis and Listeria monocytogenes were found to be the most resistant bacteria to the T. spicata L. essential oil (0.625%). The oil sample showed high bactericidal effect against all test microorganisms (0.313–1.25%). Essential oil of the plant exhibited higher antimicrobial activity than the extract. As a result, T. spicata L. could be used in pharmaceutical and food industries as a natural antioxidant and in antimicrobial products.

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References

  1. M.M. Tajkarimi, S.A. Ibrahim, D.O. Cliver, Antimicrobial herb and spice compounds in food. Food Control 21(9), 1199–1218 (2010)

    CAS  Google Scholar 

  2. R. Gyawali, S.A. Ibrahim, Natural products as antimicrobial agents. Food Control 46, 412–429 (2014)

    CAS  Google Scholar 

  3. Y. Zhang, X. Liu, Y. Wang, P. Jiang, S. Quek, Antibacterial activity and mechanism of cinnamon essential oil against Escherichia coli and Staphylococcus aureus. Food Control 59, 282–289 (2016)

    CAS  Google Scholar 

  4. B.A. Wafa, M. Makni, S. Ammar, L. Khannous, A.B. Hassana, M. Bouaziz, N.E. Es-Safi, R. Gdoura, Antimicrobial effect of the tunisian nana variety Punica granatum L. extracts against salmonella enterica (serovars Kentucky and Enteritidis) isolated from chicken meat and phenolic composition of its peel extract. Int. J. Food Microbiol. 241, 123–131 (2017)

    CAS  PubMed  Google Scholar 

  5. D.D. Jayasena, C. Jo, Essential oils as potential antimicrobial agents in meat and meat products: a review. Trends Food Sci. Tech. 34(2), 96–108 (2013)

    CAS  Google Scholar 

  6. H. Sakkas, C. Papadopoulou, Antimicrobial activity of basil, oregano, and thyme essential oils. J. Microbiol. Biotechnol. 27(3), 429–438 (2017)

    CAS  PubMed  Google Scholar 

  7. S.A. Hayek, R. Gyawali, S.A. Ibrahim, Antimicrobial natural products. Formatex Info. 27, 910–921 (2013)

    Google Scholar 

  8. C. Cueva, M.V. Moreno-Arribas, P.J. Martín-Álvarez, G. Bills, M.F. Vicente, A. Basilio, C.L. Rivas, T. Requena, J.M. Rodríguez, B. Bartolomé, Antimicrobial activity of phenolic acids against commensal probiotic and pathogenic bacteria. Res. Microbiol. 161(5), 372–382 (2010)

    CAS  PubMed  Google Scholar 

  9. P.K. Lai, J. Roy, Antimicrobial and chemopreventive properties of herbs and spices. Curr. Med. Chem. 11, 1451–1460 (2004)

    CAS  PubMed  Google Scholar 

  10. J. Xue, P.M. Davidson, Q. Zhong, Thymol nanoemulsified by whey protein-maltodextrin conjugates: the enhanced emulsifying capacity and antilisterial properties in milk by propylene glycol. J. Agric. Food Chem. 61(51), 12720–12726 (2013)

    CAS  PubMed  Google Scholar 

  11. S. Kizil, N. Hasimi, V. Tolan, Biological activities of Origanum, Satureja, Thymbra and Thymus species grown in Turkey. J. Essent. Oil Bear. Plant 17(3), 460–468 (2014)

    CAS  Google Scholar 

  12. K. Radhakrishnan, S. Babuskin, P.A.S. Babu, M. Sasikala, K. Sabina, G. Archana, M. Sivarajan, M. Sukumar, Antimicrobial and antioxidant effects of spice extracts on the shelf life extension of raw chicken meat. Int. J. Food Microbiol. 171, 32–40 (2014)

    CAS  Google Scholar 

  13. L. Guerra-Boone, R. Alvarez-Román, R. Salazar-Aranda, A. Torres-Cirio, V.M. Rivas-Galindo, N. Waksman de Torres, G. González, L.A. Pérez-López, Antimicrobial and antioxidant activities and chemical characterization of essential oils of Thymus vulgaris, Rosmarinus officinalis, and Origanum majorana from northeastern México. Pak. J. Pharm. Sci. 28(1), 363–369 (2015)

    CAS  PubMed  Google Scholar 

  14. C. Sarikurkcu, G. Zengin, M. Oskay, S. Uysal, R. Ceylan, A. Aktumsek, Composition, antioxidant, antimicrobial and enzyme inhibition activities of two Origanum vulgare subspecies (subsp. vulgare and subsp. hirtum) essential oils. Ind. Crops Prod. 70, 178–184 (2015)

    CAS  Google Scholar 

  15. J. Delgado-Adámez, M. Garrido, M.E. Bote, M.C. Fuentes-Pérez, J. Espino, D. Martín-Vertedor, Chemical composition and bioactivity of essential oils from flower and fruit of Thymbra capitata and Thymus species. J. Food Sci. Technol. 54(7), 1857–1865 (2017)

    PubMed  PubMed Central  Google Scholar 

  16. S. Albayrak, A. Aksoy, Phenolic contents and biological activity of endemic Origanum minutiflorum grown in Turkey. Indian J. Pharm. Edu. Res. 53(1), 160–170 (2019)

    CAS  Google Scholar 

  17. Z.K. Emiroglu, G.P. Yemis, B.K. Coskun, K. Candogan, Antimicrobial activity of soy edible films incorporated with thyme and oregano essential oils on fresh ground beef patties. Meat Sci. 86(2), 283–288 (2010)

    CAS  PubMed  Google Scholar 

  18. D. Dussault, K.D. Vu, M. Lacroix, In vitro evaluation of antimicrobial activities of various commercial essential oils, oleoresin and pure compounds against food pathogens and application in ham. Meat Sci. 96(1), 514–520 (2014)

    CAS  PubMed  Google Scholar 

  19. K. Bhargava, D.S. Conti, S.R. da Rocha, Y. Zhang, Application of an oregano oil nanoemulsion to the control of foodborne bacteria on fresh lettuce. Food Microbiol. 47, 69–73 (2015)

    CAS  PubMed  Google Scholar 

  20. S. Van Haute, K. Raes, P. Van der Meeren, I. Sampers, The effect of cinnamon, oregano and thyme essential oils in marinade on the microbial shelf life of fish and meat products. Food Control 68, 30–39 (2016)

    Google Scholar 

  21. C. Agrimonti, J.C. White, S. Tonetti, N. Marmiroli, Antimicrobial activity of cellulosic pads amended with emulsions of essential oils of oregano, thyme and cinnamon against microorganisms in minced beef meat. Int. J. Food Microbiol. 305, 108246 (2019)

    CAS  PubMed  Google Scholar 

  22. A. Akgul, M. Ozcan, F. Chialva, F. Monguzzi, Essential oils of four turkish wild-growing labiatae herbs: Salvia cryptantha Montbr et Auch, Satureja cuneifolia Ten, Thymbra spicata L. and Thymus cilicicus Boiss. Et. Bal. J. Essent. Oil Res. 11(2), 209–214 (1999)

    CAS  Google Scholar 

  23. A. Barakat, L.H. Wakim, N.A. Apostolides, G. Srour, M. ElBeyrouthy, Variation in the essential oils of Thymbra spicata L. growing wild in Lebanon according to the date of harvest. J. Essent. Oil Res. 25(6), 506–511 (2013)

    CAS  Google Scholar 

  24. F. Mueller-Riebau, B. Berger, O. Yegen, Chemical composition and fungitoxic properties to phytopathogenic fungi of essential oils of selected aromatic plants growing wild in Turkey. J Agric. Food Chem. 43, 2262–2266 (1995)

    CAS  Google Scholar 

  25. T. Markovıć, P. Chatzopoulou, J. Šiljegović, M. Nikolić, J. Glamočlija, A. Ćirić, M. Soković, Chemical analysis and antimicrobial activities of the essential oils of Satureja thymbra L. and Thymbra spicata L. and their main components. Arch. Biol. Sci. 63(2), 457–464 (2011)

    Google Scholar 

  26. N. Sarac, A. Ugur, M.E. Duru, Antimicrobial activity and chemical composition of the essential oils of Thymbra spicata var. intricata. Int. J. Green Pharm 3, 24–28 (2009)

    Google Scholar 

  27. H. Baydar, O. Sagdic, G. Ozkan, T. Karadogan, Antibacterial activity and composition of essential oils from Origanum, Thymbra and Satureja species with commercial importance in Turkey. Food Control 15(3), 169–172 (2004)

    CAS  Google Scholar 

  28. T. Kilic, Analysis of essential oil composition of Thymbra spicata var spicata: antifungal, antibacterial and antimycobacterial activities. Z. Naturforsch. C. 61(5–6), 324–328 (2006)

    CAS  PubMed  Google Scholar 

  29. M. Unlu, G. Vardar-Unlu, N. Vural, E. Donmez, Z.Y. Ozbas, Chemical composition, antibacterial and antifungal activity of the essential oil of Thymbra spicata L. from Turkey. Nat. Prod. Res. 23(6), 572–579 (2009)

    CAS  PubMed  Google Scholar 

  30. M. Iriti, S. Vitalini, N. Arnold Apostolides, M. El Beyrouthy, Chemical composition and antiradical capacity of essential oils from Lebanese medicinal plants. J. Essent. Oil Res. 26(6), 466–472 (2014)

    CAS  Google Scholar 

  31. M.M. Ozcan, G. Ozkan, O. Sagdic, Antibacterial effects of Istanbul thyme (Origanum vulgare L. subsp hirtum (Link) Ietsw.) and Karabas thyme (Thymbra spicata L. va spicata) extracts. Arch Lebensmittelhyg. 66, 177–181 (2015)

    Google Scholar 

  32. European Pharmacopoeia, Vol 3, pp. 68, Maissonneuve SA, Sainte-Ruffine, (1975)

  33. M.C. Scur, F.G.S. Pintoa, J.A. Pandinia, W.F. Costab, C.W. Leiteb, L.G. Temponi, Antimicrobial and antioxidant activity of essential oil and different plant extracts of Psidium cattleianum Sabine. Brazil. J. Biol. 76(1), 101–108 (2016)

    CAS  Google Scholar 

  34. USP, The U.S. Pharmacopeia National Formularty. USP 23 NF 18, pp. 1755, (1995)

  35. V.L. Singleton, J.A. Rossi, Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J. Enol Vitic 16, 144–158 (1965)

    CAS  Google Scholar 

  36. D.G. Naik, C.N. Dandge, S.V. Rupanar, Chemical examination and evaluation of antioxidant and antimicrobial activities of essential oil from Gymnema sylvestre R. Br. leaves. J. Essent. Oil Res. 23(3), 12–19 (2011)

    CAS  Google Scholar 

  37. R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang, C. Rice-Evans, Antioxidant activity applying an improved ABTS radical cation decolarization assay. Free Radical Biol. Med. 26, 1231–1237 (1999)

    CAS  Google Scholar 

  38. Y. Deng, G. Yang, J. Yue, B. Qian, Z. Liu, D. Wang, Y. Zhong, Y. Zhao, Influences of ripening stages and extracting solvents on the polyphenolic compounds, antimicrobial and antioxidant activities of blueberry leaf extracts. Food Control 38, 184–191 (2014)

    CAS  Google Scholar 

  39. SPSS, Statistical Package, SPSS for Windows, Ver. 20.0, Chicago (2011)

  40. M. AlHafi, M. ElBeyrouthy, N. Ouaini, D. Stien, D. Rutledge, S. Chaillou, Chemical composition and antimicrobial activity of Satureja, Thymus, and Thymbra species grown in Lebanon. Chem. Biodiversity 14(5), e1600236 (2017)

    Google Scholar 

  41. O. Erturk, G.I. Tanrikulu, C. Yavuz, Z. Can, H. Ebru, Chemical compositions, antioxidant and antimicrobial activities of the essential oil and extracts of Lamiaceae family (Ocimum basilicum and Thymbra spicata) from Turkey. Int J Sec Metab. 4(2), 340–348 (2017)

    Google Scholar 

  42. T. Gumus, S. Albayrak, O. Sagdic, M. Arici, Effect of gamma irradiation on total phenolic contents and antioxidant activities of Satureja hortensis, Thymus vulgaris, and Thymbra spicata from Turkey. Int. J. Food Prop. 14(4), 830–839 (2011)

    CAS  Google Scholar 

  43. M.M. Ozcan, G. Ozkan, The total phenol, flavonol amounts, antioxidant capacity and antiradical activity of some thyme species growing in Turkey. J. Chilean Chem. Society 63(3), 4051–4056 (2018)

    CAS  Google Scholar 

  44. R. Huang, E. Mendis, S.K. Kim, Factors affecting the free radical scavenging behavior of chitosan sulfate. Int. J. Biol. Macromol. 36, 120–127 (2005)

    CAS  PubMed  Google Scholar 

  45. A. Ray, S. Jena, B. Dash, B. Kar, T. Halder, T. Chatterjee, B. Ghosh, P.C. Panda, S. Nayak, N. Mahapatra, Chemical diversity, antioxidant and antimicrobial activities of the essential oils from Indian populations of Hedychium coronarium Koen. Ind. Crops Prod. 112, 353–362 (2018)

    CAS  Google Scholar 

  46. E. Buyuktuncel, Main spectrophotometric methods for the determination of total phenolic content and antioxidant capacity. Marmara Pharm. J. 17, 93–103 (2013)

    CAS  Google Scholar 

  47. S.C. Liu, J.T. Lin, C.K. Wang, Antioxidant properties of various solvent extracts from lychee (Litchi chinenesis Sonn.) flowers. Food Chem. 114, 577–581 (2009)

    CAS  Google Scholar 

  48. L.N. Vhangani, J. Van Wyk, Antioxidant activity of Maillard reaction products (MRPs) in a lipid-rich model system. Food Chem. 208, 301–308 (2016)

    CAS  PubMed  Google Scholar 

  49. I. Gulcin, Z. Huyut, M. Elmastas, H.Y. Aboul-Enein, Radical scavenging and antioxidant activity of tannic acid. Arab. J. Chem. 3(1), 43–53 (2010)

    CAS  Google Scholar 

  50. I. Kilic, Y. Yesiloglu, Spectroscopic studies on the antioxidant activity of p-coumaric acid. Spectrochim. Acta Part A 115, 719–724 (2013)

    CAS  Google Scholar 

  51. Y. Erturk, S. Ercisli, M. Sengul, Z. Eser, A. Haznedar, M. Turan, Seasonal variation of total phenolic, antioxidant activity and minerals in fresh tea shoots (Camellia sinensis var. sinensis). Pak. J. Pharm. Sci. 23(1), 69–74 (2010)

    CAS  PubMed  Google Scholar 

  52. M. Roy, A. Sarker, M.A.K. Azad, M.R. Shaheb, M.M. Hoque, Evaluation of antioxidant and antimicrobial properties of dark red kidney bean (Phaseolus vulgaris) protein hydrolysates. J. Food Meas. Charact. 14(1), 303–313 (2020)

    Google Scholar 

  53. M. Hyldgaard, T. Mygind, R.L. Meyer, Essential oils in food preservation: mode of action, synergies, and interactions with food matrix components. Front. Microbiol. 3, 12 (2012)

    PubMed  PubMed Central  Google Scholar 

  54. F. Deba, T.D. Xuan, M. Yasuda, S. Tawata, Chemical composition and antioxidant, antibacterial and antifungal activities of the essential oils from Bidens pilosa Linn. Var. Radiata. Food Control 19(4), 346–352 (2008)

    CAS  Google Scholar 

  55. S.R. Macwan, B.K. Dabhi, K.D. Aparnathi, J.B. Prajapati, Essential oils of herbs and spices: their antimicrobial activity and application in preservation of food. Int. J. Curr. Microbiol. Appl. Sci. 5(5), 885–901 (2016)

    CAS  Google Scholar 

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Authors and Affiliations

  1. Food Engineering Department, Engineering Faculty, Ege University, 35100, Bornova, Izmir, Turkey

    Ilkin Yucel Sengun, Berna Ozturk & Gulden Kilic

  2. Biology Department, Science Faculty, Eskisehir Technical University, Eskisehir, Turkey

    Ersin Yucel

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  1. Ilkin Yucel Sengun
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Sengun, I.Y., Yucel, E., Ozturk, B. et al. Chemical compositions, total phenolic contents, antimicrobial and antioxidant activities of the extract and essential oil of Thymbra spicata L. growing wild in Turkey. Food Measure 15, 386–393 (2021). https://doi.org/10.1007/s11694-020-00639-2

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