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A fluorescence nanobiosensor for detection of Campylobacter jejuni DNA in milk based on Au/Ag bimetallic nanoclusters

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Abstract

We designed a rapid method for DNA sensing for detection of campylobacter jejuni based on a selective DNA probe. A sequence containing 12 cytosine nucleotides was applied as a scaffold for making Au/Ag nanocluster. This sequence along with a 30 nucleotides selective probe was used for campylobacter jejuni DNA detection. The Au/Ag nanocluster showed a turn-off fluorescence response in presence of target DNA and probe/target hybridization process. Our result showed that with gradual increase in target concentration, intensity of fluorescence sequentially decreased. Increase in target concentration from 10 to 300 pM showed linear decrease in Au/Ag nanocluster fluorescence emission. Limit of detection of this method was 4.4 pM and capability of this method in detection of selective target from non-selective DNA was considerable. This nanobiosensor was used for evaluation of bacterial target DNA spiked in milk sample and results indicated applicability of this structure for bacterial detection in medical, environmental and food samples.

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Acknowledgements

The authors thank the research Council of University of Tehran (Grant 28645/01/02) and Tehran University of Medical Sciences for financial support of this work.

Funding

Financial support was provided by the Faculty of new sciences and technologies, Tehran University, Tehran (Iran).

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Authors and Affiliations

  1. Department of Life Science Engineering, Faculty of New Sciences & Technologies, University of Tehran, Tehran, Iran

    Zahra Dehghani, Morteza Hosseini & Javad Mohammadnejad

  2. Department of Pharmaceutical Biomaterials and Medical Biomaterials Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

    Morteza Hosseini

Authors
  1. Zahra Dehghani
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  2. Morteza Hosseini
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  3. Javad Mohammadnejad
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Correspondence to Morteza Hosseini.

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Dehghani, Z., Hosseini, M. & Mohammadnejad, J. A fluorescence nanobiosensor for detection of Campylobacter jejuni DNA in milk based on Au/Ag bimetallic nanoclusters. Food Measure 13, 1797–1804 (2019). https://doi.org/10.1007/s11694-019-00098-4

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