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LIBS based spectroscopic analysis and antidiabetic evaluation of a polyherbal formulation

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Abstract

Although plants have long been a major source of medicine, there is renewed interest in studying the phytochemistry and use of herbal formulations. This paper reports spectroscopic analysis using Laser Induced Breakdown Spectroscopy (LIBS) of a polyherbal formulation, whose antidiabetic activity has also been demonstrated on rat models. LIBS analysis revealed the presence of elements such as Na, K, Mg, Ca, H, O and N. The antidiabetic study showed that amongst the four doses studied (50, 100, 150 and 200 mg/kg bw), the dose of 150 mg/kg bw registered the maximum fall in Blood Glucose Level (BGL) in both normal and diabetic (sub and mild) rats in the Glucose Tolerance Test (GTT) study—normal rats (22 %), sub-diabetic (36.6 %) and mild-diabetic (39 %). The dose of 150 mg/kg also showed the maximum fall of 23.7 % and 22 % in BGL during fasting BGL and GTT studies of normal rats, respectively. The formulation also showed significant antioxidant activity assessed using in vitro assays. The study validates for the first time the therapeutic use of an antidiabetic polyherbal formulation.

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References

  1. R. Gupta, B. Gabrielsen, F.M. Ferguson, Curr. Drug Discov. Technol. 2, 203–219 (2005)

    Article  CAS  Google Scholar 

  2. M.A.L. Dubois, A.C.M. Offer, Phytochem. Rev. 4, 139–149 (2005)

    Article  Google Scholar 

  3. V.K. Dua, G. Verma, D.D. Agarwal, M. Kaiser, R. Brun, J. Ethnopharmacol. 136, 123–128 (2011)

    Article  Google Scholar 

  4. S.K. Mitra, S. Gopumadhavan, T.S. Hemavathi, T.S. Muralidhar, M.V. Venkataranganna, J. Ethnopharmacol. 52, 165–169 (1996)

    Article  CAS  Google Scholar 

  5. P. Bafna, S. Bodhankar, J. Ethnopharmacol. 86, 173–176 (2003)

    Article  Google Scholar 

  6. B.Y. Kang, S.W. Chung, S.H. Kim, S.Y. Ryu, T.S. Kim, Immunol. Pharmacol. 49, 355–361 (2000)

    Article  CAS  Google Scholar 

  7. P. Banerjee, S. Maity, T. Das, S. Mazumder, J. Ethnopharmacol. 134, 429–433 (2011)

    Article  Google Scholar 

  8. M.Z. Hossain, B.A. Shibib, R. Rahman, Indian J. Exp. Biol. 30, 418–420 (1992)

    CAS  Google Scholar 

  9. M. Heinrich, J. Barnes, S. Gibbons, E.M. Williamson, Fundamentals of Pharmacognosy and Phytotherapy (Churchill Livingstone, Elsevier Science Ltd., U.K., 2004), pp. 48–59

  10. A.K. Pathak, R. Kumar, V.K. Singh, R. Agrawal, S. Rai, A.K. Rai, Appl. Spectrosc. Rev. 47, 14–40 (2012)

    Article  CAS  Google Scholar 

  11. P.K. Rai, S. Chatterji, N.K. Rai, D. Bicanic, A.K. Rai, G. Watal, Food Biophys. 5, 17–23 (2009)

    Article  Google Scholar 

  12. P.K. Rai, D. Jaiswal, N.K. Rai, S. Pandhija, A.K. Rai, G. Watal, Lasers Med. Sci. 24, 761–768 (2009)

    Google Scholar 

  13. C.J. Bailey, Trends Pharmacol. Sci. 21, 259–265 (2000)

    Article  CAS  Google Scholar 

  14. K.A. Abo, A.A. Fred-Jaiyesimi, J. Ethnopharmacol. 115, 67–71 (2008)

    Article  CAS  Google Scholar 

  15. S. Bastaki, Int. J. Diab. Metabol. 13, 111–134 (2005)

    Google Scholar 

  16. D. Brahm, P. Trinder, Analyst 97, 142–145 (1972)

    Article  Google Scholar 

  17. P.A. Hammerschmidt, D.E. Pratt, J. Food Sci. 43, 556–569 (1978)

    Article  CAS  Google Scholar 

  18. A.A.L. Ordon Ez, J.D. Gomez, M.A. Vattuone, M.I. Isla, Food. Chem. 97, 452–458 (2006)

    Google Scholar 

  19. A. Kumaran, R.J. Karunakaran, Lebens-Wiss Technol. 40, 344–352 (2007)

    Article  CAS  Google Scholar 

  20. I.F.F. Benzie, J.J. Strain, Anal. Biochem. 239, 70–76 (1996)

    Article  CAS  Google Scholar 

  21. A. Lozak, K. Soltyk, P. Ostapczuk, Sci. Total Environ. 289, 33–40 (2002)

    Article  CAS  Google Scholar 

  22. M. Mertz, Physiol. Rev. 49, 163–169 (1969)

    CAS  Google Scholar 

  23. A. Abo, A.A. Fred-Jaiyesimi, A.E.A. Jaiyesimi, Afr. J. Med. Sci. 29, 325–327 (2007)

    Google Scholar 

  24. M. Hoenig, A. Kersabiec, Spectrochim. Acta Part B: At. Spectrosc. 51, 1297–1307 (1996)

    Article  Google Scholar 

  25. A.K. Rai, H. Zhang, F.U. Yueh, J.P. Singh, A. Spectrochim. Acta Part B: At. Spectrosc. 56, 2371–2383 (2001)

    Article  Google Scholar 

  26. E.J. Underwood, W. Mertz, Trace Elements in Human and Animal Nutrition, vol. 1 (Academic Press, New York, 1986), p. 255

  27. G. Watal, B. Sharma, P.K. Rai, D. Jaiswal, D.K. Rai, N.K., Rai, A.K., Rai, Adv. Prot. Oxid. Stress. Meth. Mol. Biol. Ser. 594, 275–285 (2010)

  28. S. Mehta, P.K. Rai, D.K. Rai, N.K. Rai, A.K. Rai, B. Sharma, D. Bicanic, G. Watal, Food Biophys. 5, 186–192 (2010)

    Article  Google Scholar 

  29. H.M. Mather, J.A. Nisbet, G.H. Burton, G.J. Poston, J.M. Bland, P.A. Bailey, T.R. Pilkington, Clin. Chim. Acta. 95, 235–242 (1979)

    Article  CAS  Google Scholar 

  30. G.J.N. Raju, P. Sarita, A.V. Ramana, M.R. Kumar, B. Seetharami, M. Reddy, J. Charles, et al., Appl. Rad. Isotop. 64, 893–900 (2006)

    Google Scholar 

  31. H.H. Zaman, P.T.G. Lian, S. Hussain, R. Pendek, R.T. Widodo, Int. J. Diab. Metab. 17, 9–15 (2009)

    Google Scholar 

  32. A. Ceriello, Metabolism 49, 27–29 (2000)

    Article  CAS  Google Scholar 

  33. J.W. Baynes, S.R. Thorpe, Diabetes 48, 1–9 (1999)

    Article  CAS  Google Scholar 

  34. J.W. Baynes, Diabetes 40, 405–412 (1991)

  35. B. Halliwell, J.M. Gutteridge, Methods Enzymol. 186, 1–85 (1990)

    Article  CAS  Google Scholar 

  36. E.C. Tsai, I.B. Hirsch, J.D. Brunzell, A. Chait, Diabetes 43, 1010–1014 (1994)

    Article  CAS  Google Scholar 

  37. P.K. Rai, D. Jaiswal, D.K. Rai, B. Sharma, G. Watal, J. Food Biochem. 24, 78–92 (2010)

    Article  Google Scholar 

Download references

Acknowledgments

PKR and SG are supported by National Medicinal Plants Board (NMPB), Department of AYUSH, Ministry of Health & Family Welfare, Government of India. Mr. Ashok Kumar Pathak is supported by Teacher Fellowship Award under FIP Scheme [(No. F.27-99 (TF)/2009(NRCB)], University Grants Commission (UGC), New Delhi, India.

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Authors and Affiliations

  1. Department of NMR, All India Institute of Medical Sciences, New Delhi, 110 029, India

    Prashant Kumar Rai, Somenath Ghatak & Rama Jayasundar

  2. Department of Chemistry, University of Allahabad, Allahabad, India

    Geeta Watal

  3. Department of Physics, University of Allahabad, Allahabad, India

    Ashok Kumar Pathak & Awadhesh Kumar Rai

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  1. Prashant Kumar Rai
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  2. Ashok Kumar Pathak
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  3. Somenath Ghatak
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Correspondence to Rama Jayasundar.

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Rai, P.K., Pathak, A.K., Ghatak, S. et al. LIBS based spectroscopic analysis and antidiabetic evaluation of a polyherbal formulation. Food Measure 7, 114–121 (2013). https://doi.org/10.1007/s11694-013-9146-3

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  • DOI: https://doi.org/10.1007/s11694-013-9146-3

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