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Safety of Three-Dimensional Conformal Radiotherapy on the Sheep with Pulmonary Cystic Echinococcosis: A Preliminary Study

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Abstract

Purpose

Radiotherapy showed the potential to effectively kill the cysts of pulmonary cystic echinococcosis (CE). However, little is known about its safety. This study was designed to investigate the safety of three-dimensional conformal radiotherapy (3D-CRT) on the normal lung tissue adjacent to the cyst and blood of sheep naturally infected with pulmonary CE.

Methods

Twenty pulmonary CE sheep were randomly divided into control group (n = 5) and radiation groups with a dose of 30 Gray (Gy) (n = 5), 45 Gy (n = 5), and 60 Gy (n = 5), respectively. Animals in control group received no radiation. Heat shock protein 70 (Hsp70), tumor growth factor-β (TGF-β), matrix metalloproteinase-2 (MMP-2) and MMP-9 in the lung tissues adjacent to the cysts, which were considered to be closely related to the pathogenesis of CE, were evaluated after 3D-CRT. A routine blood test was conducted.

Results

The results showed that there were multiple cysts of various sizes with protoscoleces in the lung tissues of sheep, and necrotic cysts were found after 3D-CRT. 3D-CRT significantly increased the mRNA level of Hsp70, enhanced the protein level of TGF-β and slightly increased the expression of MMP-2 and MMP-9 in lung tissues adjacent to the cysts. 3D-CRT did not significantly alter the amount of WBC, HB and PLT in sheep blood.

Conclusions

The results suggested that 3D-CRT may suppress the inflammation and induce less damage of the normal lung tissues and blood. We preliminarily showed that 3D-CRT under a safe dose may be used to treat pulmonary CE.

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Data Availability

The data sets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

References

  1. McManus DP, Gray DJ, Zhang W, Yang Y (2012) Diagnosis, treatment, and management of echinococcosis. BMJ 344:e3866. https://doi.org/10.1136/bmj.e3866

    Article  PubMed  Google Scholar 

  2. Cadavid Restrepo AM, Yang YR, McManus DP, Gray DJ, Giraudoux P, Barnes TS et al (2016) The landscape epidemiology of echinococcoses. Infect Dis Poverty 5:13. https://doi.org/10.1186/s40249-016-0109-x

    Article  PubMed  PubMed Central  Google Scholar 

  3. Lashkarizadeh MR, Hooshmand N, Nasibi S, Mohammadi MA, Shamsaddini S, Kamyabi H et al (2019) Genetic profile of hydatid cysts in patients with multi-organ involvement: mixed infections by different strains. Vector Borne Zoonotic Dis 19(10):724–730. https://doi.org/10.1089/vbz.2018.2427

    Article  PubMed  Google Scholar 

  4. Ding NH, Li JJ, Sun LQ (2013) Molecular mechanisms and treatment of radiation-induced lung fibrosis. Curr Drug Targets 14(11):1347–1356. https://doi.org/10.2174/13894501113149990198

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Ozdol C, Yildirim AE, Daglioglu E, Divanlioglu D, Erdem E, Belen D (2011) Alveolar hydatid cyst mimicking cerebellar metastatic tumor. Surg Neurol Int 2:13. https://doi.org/10.4103/2152-7806.76281

    Article  PubMed  PubMed Central  Google Scholar 

  6. Dominique R, Francesca T, Renato U, Silivia B, Amedea M, Silvio Q (2002) Terpyridine Zn (II), Ru (III) and Ir (III) complexex as new asymmetric chromophores for nonlinear optics: first evidence for a shift from positive to negative value of the quadratic hyperpolarizability of a ligand carrying an electron donor substituent upon coordination to different metal centres. Chem Commun (Camb) 8:846–847. https://doi.org/10.1039/b201221a

    Article  CAS  Google Scholar 

  7. Aasen TD, Nasrollah L, Seetharam A (2018) Drug-induced liver failure requiring liver transplant: report and review of the role of albendazole in managing echinococcal infection. Exp Clin Transplant 16(3):344–347. https://doi.org/10.6002/ect.2015.0313

    Article  PubMed  Google Scholar 

  8. Engels E, Li N, Davis J, Paino J, Cameron M, Dipuglia A et al (2020) Toward personalized synchrotron microbeam radiation therapy. Sci Rep 10(1):8833. https://doi.org/10.1038/s41598-020-65729-z

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Mao R, Wu G, Wang H, Lu P, Li J, Li H et al (2017) Effects of X-ray on the metacestodes of Echinococcus granulosus in vitro. BMC Infect Dis 17(1):636. https://doi.org/10.1186/s12879-017-2741-x

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Zhang Y, Lu P, Qi H, Wu G, Mao R, Bao Y (2021) Radiotherapy for the treatment of pulmonary hydatidosis in sheep. Open Life Sci 16(1):1405–1411. https://doi.org/10.1515/biol-2021-0139

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Zhang YF, Xie ZR, Ni YQ, Mao R, Qi HZ, Yang YG et al (2011) Curative effect of radiotherapy at various doses on subcutaneous alveolar echinococcosis in rats. Chin Med J (Engl) 124(18):2845–2848

    PubMed  Google Scholar 

  12. Fernández-Fernández MR, Valpuesta JM (2018) Hsp70 chaperone: a master player in protein homeostasis. F1000Res. https://doi.org/10.12688/f1000research.15528.1

    Article  PubMed  PubMed Central  Google Scholar 

  13. Ortona E, Margutti P, Delunardo F, Vaccari S, Riganò R, Profumo E et al (2003) Molecular and immunological characterization of the C-terminal region of a new Echinococcus granulosus Heat Shock Protein 70. Parasite Immunol 25(3):119–126. https://doi.org/10.1046/j.1365-3024.2003.00617.x

    Article  CAS  PubMed  Google Scholar 

  14. Meng XM, Nikolic-Paterson DJ, Lan HY (2016) TGF-β: the master regulator of fibrosis. Nat Rev Nephrol 12(6):325–338. https://doi.org/10.1038/nrneph.2016.48

    Article  CAS  PubMed  Google Scholar 

  15. Batlle E, Massagué J (2019) Transforming growth factor-β signaling in immunity and cancer. Immunity 50(4):924–940. https://doi.org/10.1016/j.immuni.2019.03.024

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Díaz Á (2017) Immunology of cystic echinococcosis (hydatid disease). Br Med Bull 124(1):121–133. https://doi.org/10.1093/bmb/ldx033

    Article  CAS  PubMed  Google Scholar 

  17. Mirzavand S, Rafiei A, Teimoori A, Khorsandi L, Bahreini A, Motamedfar A et al (2020) Gene expression in human liver fibrosis associated with Echinococcus granulosus sensu lato. Parasitol Res 119(7):2177–2187. https://doi.org/10.1007/s00436-020-06700-9

    Article  PubMed  Google Scholar 

  18. Sokouti M, Sokouti M, Sokouti B (2001) Structure of the pulmonary hydatid cyst. J Med Ultrason 41(2):251–252. https://doi.org/10.1007/s10396-013-0492-x

    Article  Google Scholar 

  19. Kim JY, Choeng HC, Ahn C, Cho SH (2009) Early and late changes of MMP-2 and MMP-9 in bleomycin-induced pulmonary fibrosis. Yonsei Med J 50(1):68–77. https://doi.org/10.3349/ymj.2009.50.1.68

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Corbel M, Belleguic C, Boichot E, Lagente V (2002) Involvement of gelatinases (MMP-2 and MMP-9) in the development of airway inflammation and pulmonary fibrosis. Cell Biol Toxicol 18(1):51–61. https://doi.org/10.1023/a:1014471213371

    Article  CAS  PubMed  Google Scholar 

  21. Marco M, Baz A, Fernandez C, Gonzalez G, Hellman U, Salinas G et al (2006) A relevant enzyme in granulomatous reaction, active matrix metalloproteinase-9, found in bovine Echinococcus granulosus hydatid cyst wall and fluid. Parasitol Res 100(1):131–139. https://doi.org/10.1007/s00436-006-0237-5

    Article  CAS  PubMed  Google Scholar 

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Funding

This study was supported by the National Natural Science Foundation (Nos. 81860556 and 81860360) and the State Key Laboratory and the High Incidence Disease of Central Asia (SKL-HIDCA-2019-29).

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Authors and Affiliations

  1. State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Department of Radiation Oncology, The First Affiliated Hospital of Xinjiang Medical University, No. 137 South Liyushan Road, Urumqi, 830054, China

    Yuefen Zhang, Pengfei Lu, Hongzhi Qi, Rui Mao & Yongxing Bao

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  1. Yuefen Zhang
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  2. Pengfei Lu
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  3. Hongzhi Qi
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  4. Rui Mao
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Contributions

Data curation: ZYF, LPF and QHZ; funding acquisition: MR, QHZ and ZYF; conceptualization: MR and BYX; writing—original draft: ZYF and LPF; writing—review and editing: MR and BYX. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Rui Mao or Yongxing Bao.

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The authors have no competing interests to declare that are relevant to the content of this article.

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The study protocols were approved by the Ethics Committee of the First Affiliated Hospital of Xinjiang Medical University (approval No. IACUC-2014021002).

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Zhang, Y., Lu, P., Qi, H. et al. Safety of Three-Dimensional Conformal Radiotherapy on the Sheep with Pulmonary Cystic Echinococcosis: A Preliminary Study. Acta Parasit. 69, 559–566 (2024). https://doi.org/10.1007/s11686-023-00751-5

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  • DOI: https://doi.org/10.1007/s11686-023-00751-5

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