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Description of Two Novel Henneguya (Cnidaria: Myxosporea) Infecting Curimatid Fish, Using Morphological, Histological, and Molecular Analyses

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Abstract

Purpose

We describe two new Henneguya spp. (Myxobolidae) found parasitizing Cyphocharax modestus from Pardo river, Paraná river basin, municipality of Botucatu, São Paulo State, Brazil: Henneguya fastigata n. sp. from gill lamellae and Henneguya pardensis n. sp. from gill arches. We based the descriptions on myxospore morphology, histology, and small subunit ribosomal DNA sequences.

Methods

In this investigation, 50 specimens of Cyphocharax modestus were examined between July and December 2020 for myxozoan infections. Morphological characterization was based on the mature myxospores. The small subunit ribosomal DNA sequences were used for molecular and phylogenetic study.

Results

Phylogenetic analysis provided the evolutionary position of these new species with other myxobolids species. Myxospores of the two species had similar morphology and morphometrics, but differed in spore body width and length, and ssrDNA sequences differed by 7.2%. These data supported the diagnosis of the parasites as distinct and novel species. The phylogenetic analysis showed a well-supported subclade formed by species that parasitize curimatid fishes, with Henneguya gilbert as a sister species of Henneguya fastigata n. sp., and Henneguya pardensis n. sp. as a sister species of both species.

Conclusion

Our analysis was consistent with previous studies suggesting that orders and families of the hosts are strongly correlated with phylogenetic signals in the Myxobolidae. These are the first species of myxozoans described in the Pardo river.

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Acknowledgments

The authors thank the Fundação de Apoio à Pesquisa do Estado de São Paulo—FAPESP (grants #2020/05412-9, #2019/19060-0, and #2019/26831-2) for the scientific and financial support; and Conselho Nacional de Desenvolvimento Científico e Tecnológico – CNPq (#309125/2017-0).

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Authors and Affiliations

  1. Laboratory of Parasitology of Wild Animals, Division of Parasitology, Institute of Biosciences, Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), R. Prof. Dr. Antônio Celso Wagner Zanin, 250, Rubião Junior, CEP 18618-689, Botucatu, SP, Brazil

    Diego Henrique Mirandola Dias Vieira, Rodrigo Bravin Narciso & Reinaldo José da Silva

  2. Programa de Pós-Graduação Em Análise de Sistemas Ambientais, Centro Universitário CESMAC, Maceió, AL, Brazil

    Rodney Kozlowiski de Azevedo

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  1. Diego Henrique Mirandola Dias Vieira
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Correspondence to Diego Henrique Mirandola Dias Vieira.

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Vieira, D.H.M.D., Narciso, R.B., de Azevedo, R.K. et al. Description of Two Novel Henneguya (Cnidaria: Myxosporea) Infecting Curimatid Fish, Using Morphological, Histological, and Molecular Analyses. Acta Parasit. 67, 233–243 (2022). https://doi.org/10.1007/s11686-021-00454-9

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