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Effect of BcL-2 antisense drug with different structure on the biological function of K562 cells

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Chinese Journal of Cancer Research

Abstract

Objective: To study the differences and similarities of the antisense drugs with different structures on the biological functions of K562 cells. Methods: Cytotoxic effects were measured by use of a cell viability assay. Flow cytometric analysis and agarose gel electrophoresis of DNA fragmentation were also performed. The expression level of protein was assayed by immunofluorescence using fluoresce isothiocyanate label. Results: PNA targeting the coding region of the Bcl-2 messenger RNA could effectively inhibit K562 cell viability, down-regulate the synthesis of the Bcl-2 protein and increase cell apoptosis. By 72 h after the Bcl-2 antisense PNA treatment, K562 cells showed more reduction in the level of Bcl-2 protein compared with cells treated with the antisense ODN. After treatment with 10 µmol/L of Bcl-2 antisense PNA or antisense ODN for 72 h, apoptotic rates of K562 cells were 13.15±1.13 and 11.72±1.12, respectively. Furthermore, there was significant difference in the percentage of apoptotic cells between antisense PNA group and antisense ODN group. Conclusion: The results suggest that antisense PNA targeting the coding region of Bcl-2 mRNA has better antisense effects than the antisense oligonucleotides on inducing apoptosis of K562 cells.

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Authors and Affiliations

  1. Institute of Hematology, Jinan University, 510632, Guangzhou

    Lei Xiao-yong, Zhang Huan & He Dong-mei

Authors
  1. Lei Xiao-yong
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  2. Zhang Huan
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  3. He Dong-mei
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Correspondence to Zhang Huan.

Additional information

Foundation item: This work was supported by the Key Foundation of Science & Technology Program of Guangzhou (No.2001-Z-037-01), and the Nature Science Key Foundation of Guangdong Province (No. 021195).

Biography: LEI Xiao-yong(1970–), male, associate professor, doctor of medicine, Institute of Pharmacy and Pharmacology, Nanhua University, majors in tumor pharmacology.

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Lei, Xy., Zhang, H. & He, Dm. Effect of BcL-2 antisense drug with different structure on the biological function of K562 cells. Chin. J. Cancer Res. 16, 109–112 (2004). https://doi.org/10.1007/s11670-004-0007-9

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  • DOI: https://doi.org/10.1007/s11670-004-0007-9

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